Restriction map for pF1KB6884
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BanII       BbeI        BbrPI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MfeI        MluI        MroI        MseI        MunI        
NarI        NciI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII          34  CGCG                 CG^CG
AcsI          484  GAATTC               [GA]^AATT[CT]
AfaI          207  GTAC                 GT^AC
AflIII        329  ACATGT               A^C[GA][CT]GT
AlwNI         286  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          484  GAATTC               [GA]^AATT[CT]
AvaI          238  CCCGAG               C^[CT]CG[GA]G
BalI            2  TGGCCA               TGG^CCA
BanI          234  GGTGCC               G^G[CT][GA]CC
BbsI          259  GTCTTC               GAAGAC(2/6)
BbvCI         301  GCTGAGG              CCTCAGC(-5/-2)
BglI          553  GCCTCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI           76  CTGGAG               CTGGAG(16/14)
BpuAI         259  GTCTTC               GAAGAC(2/6)
BsoBI         238  CCCGAG               C^[CT]CG[GA]G
BspLU11I      329  ACATGT               A^CATGT
BspMI         276  GCAGGT               ACCTGC(4/8)
BsrBI          82  GAGCGG               CCGCTC(-3/-3)
BsrI          449  ACTGG                ACTGG(1/-1)
BstUI          34  CGCG                 CG^CG
BstYI         441  AGATCC               [GA]^GATC[CT]
DrdI          307  GACTATGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EcoRI         484  GAATTC               G^AATTC
EcoT14I       254  CCATGG               C^C[AT][AT]GG
HaeII         143  GGCGCT               [GA]GCGC^[CT]
HincII        343  GTTGAC               GT[CT]^[GA]AC
HindII        343  GTTGAC               GT[CT]^[GA]AC
HinfI         180  GATTC                G^A[ACGT]TC
MaeIII        412  GTGAC                ^GT[ACGT]AC
MflI          441  AGATCC               [GA]^GATC[CT]
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MslI          430  CACTACCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        499  CAGCTG               C[AC]G^C[GT]G
MvnI           34  CGCG                 CG^CG
NaeI          562  GCCGGC               GCC^GGC
NcoI          254  CCATGG               C^CATGG
NgoMIV        562  GCCGGC               G^CCGGC
PflMI         133  CCAGACCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          502  CTGCAG               CTGCA^G
PvuII         499  CAGCTG               CAG^CTG
RsaI          207  GTAC                 GT^AC
SfcI          502  CTGCAG               C^T[GA][CT]AG
SfiI          552  GGCCTCCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI          254  CCATGG               C^C[AT][AT]GG
TaqI           53  TCGA                 T^CGA
TfiI          180  GATTC                G^A[AT]TC
Tsp45I        412  GTGAC                ^GT[GC]AC
Tsp509I       485  AATT                 ^AATT
TthHB8I        53  TCGA                 T^CGA
Van91I        133  CCAGACCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         441  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwI          442  GATCC                GGATC(4/5)
               98  GGATC               
AvaII          13  GGTCC                G^G[AT]CC
              383  GGACC               
BmyI          235  GTGCCC               G[AGT]GC[ACT]^C
              427  GGGCAC              
BseRI          70  GAGGAG               GAGGAG(10/8)
               79  GAGGAG              
Bsp1286I      235  GTGCCC               G[AGT]GC[ACT]^C
              427  GGGCAC              
BsrFI         225  GCCGGT               [GA]^CCGG[CT]
              562  GCCGGC              
BssKI         156  CCAGG                ^CC[ACGT]GG
              558  CCAGG               
BstNI         156  CCAGG                CC^[AT]GG
              558  CCAGG               
CfoI          144  GCGC                 GCG^C
              466  GCGC                
Cfr10I        225  GCCGGT               [GA]^CCGG[CT]
              562  GCCGGC              
EcoRII        156  CCAGG                ^CC[AT]GG
              558  CCAGG               
HapII         226  CCGG                 C^CGG
              563  CCGG                
HhaI          144  GCGC                 GCG^C
              466  GCGC                
HinP1I        144  GCGC                 G^CGC
              466  GCGC                
HpaII         226  CCGG                 C^CGG
              563  CCGG                
HphI          195  GGTGA                GGTGA(8/7)
              228  GGTGA               
MspI          226  CCGG                 C^CGG
              563  CCGG                
MvaI          156  CCAGG                CC^[AT]GG
              558  CCAGG               
NspI          329  ACATGT               [GA]CATG^[CT]
              516  GCATGT              
PpuMI          12  GGGTCCT              [GA]G^G[AT]CC[CT]
              382  AGGACCC             
ScrFI         156  CCAGG                CC^[ACGT]GG
              558  CCAGG               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsgI        
BsiYI       BslI        Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    Fnu4HI      
HaeIII      ItaI        MboI        MboII       MnlI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      TseI        TspRI       
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