Restriction map for pF1KB6884
 (Linear) (Six-base) MAPSORT of: KIBB6884.seq  Check: 5598  from: 1  to: 603



KIBB6884.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     267     603 
   Size:        267     336
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     313     514     603 
   Size:        187     126     201      89

  Fragments arranged by size: 

                 201     187     126      89
 
AflIII A'CryG_T

Cuts at:      0     329     603 
   Size:        329     274
 
AleI CACnn'nnGTG

Cuts at:      0     434     603 
   Size:        434     169
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     235     267     603 
   Size:        235      32     336

  Fragments arranged by size: 

                 336     235      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     603 
   Size:        291     312
 
ApoI r'AATT_y

Cuts at:      0     484     603 
   Size:        484     119
 
AvaI C'yCGr_G

Cuts at:      0     238     603 
   Size:        238     365
 
BanI G'GyrC_C

Cuts at:      0     234     603 
   Size:        234     369
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     252     603 
   Size:        252     351
 
BbvCI CC'TCA_GC

Cuts at:      0     302     603 
   Size:        302     301
 
BglI GCCn_nnn'nGGC

Cuts at:      0     559     603 
   Size:        559      44
 
Bme1580I G_kGCm'C

Cuts at:      0     239     431     603 
   Size:        239     192     172

  Fragments arranged by size: 

                 239     192     172
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     603 
   Size:         97     506
 
Bpu10I CC'TnA_GC

Cuts at:      0     302     603 
   Size:        302     301
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     235     265     603 
   Size:        235      30     338

  Fragments arranged by size: 

                 338     235      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85      94     603 
   Size:         85       9     509

  Fragments arranged by size: 

                 509      85       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     127     397     603 
   Size:         61      66     270     206

  Fragments arranged by size: 

                 270     206      66      61
 
Bsp1286I G_dGCh'C

Cuts at:      0     239     431     603 
   Size:        239     192     172

  Fragments arranged by size: 

                 239     192     172
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     267     603 
   Size:        267     336
 
BsrBI CCG'CTC

Cuts at:      0      84     603 
   Size:         84     519
 
BsrFI r'CCGG_y

Cuts at:      0     225     562     603 
   Size:        225     337      41

  Fragments arranged by size: 

                 337     225      41
 
BstYI r'GATC_y

Cuts at:      0     441     603 
   Size:        441     162
 
BtgI C'CryG_G

Cuts at:      0     138     254     435     603 
   Size:        138     116     181     168

  Fragments arranged by size: 

                 181     168     138     116
 
BtsI GCAGTG_nn'

Cuts at:      0     115     380     416     603 
   Size:        115     265      36     187

  Fragments arranged by size: 

                 265     187     115      36
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     313     603 
   Size:        313     290
 
EaeI y'GGCC_r

Cuts at:      0       2     603 
   Size:          2     601
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     187     313     514     603 
   Size:         97      90     126     201      89

  Fragments arranged by size: 

                 201     126      97      90      89
 
EcoO109I rG'GnC_Cy

Cuts at:      0      13     383     551     603 
   Size:         13     370     168      52

  Fragments arranged by size: 

                 370     168      52      13
 
EcoRI G'AATT_C

Cuts at:      0     484     603 
   Size:        484     119
 
HaeII r_GCGC'y

Cuts at:      0     147     603 
   Size:        147     456
 
HincII GTy'rAC

Cuts at:      0     345     603 
   Size:        345     258
 
MscI TGG'CCA

Cuts at:      0       4     603 
   Size:          4     599
 
MslI CAynn'nnrTG

Cuts at:      0     434     603 
   Size:        434     169
 
MspA1I CmG'CkG

Cuts at:      0     501     603 
   Size:        501     102
 
NaeI GCC'GGC

Cuts at:      0     564     603 
   Size:        564      39
 
NcoI C'CATG_G

Cuts at:      0     254     603 
   Size:        254     349
 
NgoMIV G'CCGG_C

Cuts at:      0     562     603 
   Size:        562      41
 
NspI r_CATG'y

Cuts at:      0     333     520     603 
   Size:        333     187      83

  Fragments arranged by size: 

                 333     187      83
 
PciI A'CATG_T

Cuts at:      0     329     603 
   Size:        329     274
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     139     603 
   Size:        139     464
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     235     267     603 
   Size:        235      32     336

  Fragments arranged by size: 

                 336     235      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      13     383     603 
   Size:         13     370     220

  Fragments arranged by size: 

                 370     220      13
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     190     222     603 
   Size:        190      32     381

  Fragments arranged by size: 

                 381     190      32
 
PstI C_TGCA'G

Cuts at:      0     506     603 
   Size:        506      97
 
PvuII CAG'CTG

Cuts at:      0     501     603 
   Size:        501     102
 
SfcI C'TryA_G

Cuts at:      0     502     603 
   Size:        502     101
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     559     603 
   Size:        559      44
 
StyI C'CwwG_G

Cuts at:      0     254     603 
   Size:        254     349
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AloI    AlwNI     ApoI     AvaI
     BanI     BbsI    BbvCI     BglI Bme1580I     BpmI   Bpu10I    BsaXI
    BseRI     BsgI Bsp1286I    BspMI    BsrBI    BsrFI    BstYI     BtgI
     BtsI     DrdI     EaeI  Eco57MI EcoO109I    EcoRI    HaeII   HincII
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PciI
    PflMI     PpiI    PpuMI     PsrI     PstI    PvuII     SfcI     SfiI
     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BanII     BbeI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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