Restriction map for pF1KB6860
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AfaI          339  GTAC                 GT^AC
AlwNI         256  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       176  AGCGCT               AGC^GCT
Asp700I       270  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          320  TGGCCA               TGG^CCA
BanI          225  GGCACC               G^G[CT][GA]CC
BbvCI         388  GCTGAGG              CCTCAGC(-5/-2)
BfaI           73  CTAG                 C^TAG
BglI          355  GCCTCAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmFI         164  GGGAC                GGGAC(10/14)
BspMI         260  ACCTGC               ACCTGC(4/8)
BsrBI         112  GAGCGG               CCGCTC(-3/-3)
BstYI         485  AGATCC               [GA]^GATC[CT]
DdeI          389  CTGAG                C^T[ACGT]AG
DrdI          418  GACTTCAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          320  TGGCCA               [CT]^GGCC[GA]
EarI           10  CTCTTC               CTCTTC(1/4)
Eco47III      176  AGCGCT               AGC^GCT
EcoT14I       323  CCATGG               C^C[AT][AT]GG
Ksp632I        10  CTCTTC               CTCTTC(1/4)
KspI           49  CCGCGG               CCGC^GG
MaeI           73  CTAG                 C^TAG
MaeII         167  ACGT                 A^CGT
MaeIII        416  GTGAC                ^GT[ACGT]AC
MflI          485  AGATCC               [GA]^GATC[CT]
MluNI         320  TGGCCA               TGG^CCA
MscI          320  TGGCCA               TGG^CCA
MslI          198  CACGCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          323  CCATGG               C^CATGG
PflMI         323  CCATGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         257  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          220  CTGCAG               CTGCA^G
PvuII         490  CAGCTG               CAG^CTG
RsaI          339  GTAC                 GT^AC
SacI          303  GAGCTC               GAGCT^C
SacII          49  CCGCGG               CCGC^GG
SfcI          220  CTGCAG               C^T[GA][CT]AG
SmlI          357  CTCAAG               C^T[CT][GA]AG
StyI          323  CCATGG               C^C[AT][AT]GG
Tsp45I        416  GTGAC                ^GT[GC]AC
TspRI         507  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        323  CCATGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          455  CCAGCGACTCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         485  AGATCC               [GA]^GATC[CT]
XmnI          270  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwI          171  GATCC                GGATC(4/5)
              486  GATCC               
AspHI         247  GAGCAC               G[AT]GC[AT]^C
              303  GAGCTC              
BsiHKAI       247  GAGCAC               G[AT]GC[AT]^C
              303  GAGCTC              
DpnII         171  GATC                 ^GATC
              486  GATC                
HaeII          17  GGCGCT               [GA]GCGC^[CT]
              176  AGCGCT              
HapII          58  CCGG                 C^CGG
              108  CCGG                
HgaI          236  GCGTC                GACGC(5/10)
              425  GCGTC               
HinfI         382  GACTC                G^A[ACGT]TC
              460  GACTC               
HpaII          58  CCGG                 C^CGG
              108  CCGG                
MboI          171  GATC                 ^GATC
              486  GATC                
MboII         213  GAAGA                GAAGA(8/7)
               11  TCTTC               
MspI           58  CCGG                 C^CGG
              108  CCGG                
NdeII         171  GATC                 ^GATC
              486  GATC                
PleI          382  GACTC                GAGTC(4/5)
              460  GACTC               
Sau3AI        171  GATC                 ^GATC
              486  GATC                
SfaNI         216  GATGC                GCATC(5/9)
              548  GCATC               
SmaI           57  CCCGGG               CCC^GGG
              107  CCCGGG              
XmaCI          57  CCCGGG               C^CCGGG
              107  CCCGGG              
XmaI           57  CCCGGG               C^CCGGG
              107  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AvaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BsaJI       BsiYI       BslI        BsoBI       Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DraII       DsaI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HphI        ItaI        MnlI        
MspA1I      MvaI        MvnI        MwoI        NciI        
NlaIII      NlaIV       Sau96I      ScrFI       TseI        
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