Restriction map for pF1KB6860
 (Linear) (Six-base) MAPSORT of: KIBB6860.seq  Check: 6473  from: 1  to: 561



KIBB6860.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     561 
   Size:        405     156
 
AfeI AGC'GCT

Cuts at:      0     178     561 
   Size:        178     383
 
AleI CACnn'nnGTG

Cuts at:      0     202     561 
   Size:        202     359
 
AlwNI CAG_nnn'CTG

Cuts at:      0     261     561 
   Size:        261     300
 
ApaI G_GGCC'C

Cuts at:      0      57      64      98     477     561 
   Size:         57       7      34     379      84

  Fragments arranged by size: 

                 379      84      57      34       7
 
AvaI C'yCGr_G

Cuts at:      0      57      63      87     107     561 
   Size:         57       6      24      20     454

  Fragments arranged by size: 

                 454      57      24      20       6
 
BanI G'GyrC_C

Cuts at:      0     225     561 
   Size:        225     336
 
BanII G_rGCy'C

Cuts at:      0      57      64      98     307     477     561 
   Size:         57       7      34     209     170      84

  Fragments arranged by size: 

                 209     170      84      57      34       7
 
BbvCI CC'TCA_GC

Cuts at:      0     389     561 
   Size:        389     172
 
BglI GCCn_nnn'nGGC

Cuts at:      0     361     561 
   Size:        361     200
 
Bme1580I G_kGCm'C

Cuts at:      0      57      64      98     477     561 
   Size:         57       7      34     379      84

  Fragments arranged by size: 

                 379      84      57      34       7
 
BmgBI CAC'GTC

Cuts at:      0     168     561 
   Size:        168     393
 
Bpu10I CC'TnA_GC

Cuts at:      0     389     561 
   Size:        389     172
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     342     561 
   Size:        342     219
 
BseYI C'CCAG_C

Cuts at:      0     476     521     555     561 
   Size:        476      45      34       6

  Fragments arranged by size: 

                 476      45      34       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     307     561 
   Size:        251      56     254

  Fragments arranged by size: 

                 254     251      56
 
Bsp1286I G_dGCh'C

Cuts at:      0      57      64      98     251     307     477     561 
   Size:         57       7      34     153      56     170      84

  Fragments arranged by size: 

                 170     153      84      57      56      34       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     269     561 
   Size:        269     292
 
BsrBI CCG'CTC

Cuts at:      0     114     561 
   Size:        114     447
 
BstYI r'GATC_y

Cuts at:      0     485     561 
   Size:        485      76
 
BtgI C'CryG_G

Cuts at:      0      49      81     323     464     561 
   Size:         49      32     242     141      97

  Fragments arranged by size: 

                 242     141      97      49      32
 
BtsI GCAGTG_nn'

Cuts at:      0     513     561 
   Size:        513      48
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     424     561 
   Size:        424     137
 
EaeI y'GGCC_r

Cuts at:      0     320     561 
   Size:        320     241
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     561 
   Size:         16     545
 
EcoICRI GAG'CTC

Cuts at:      0     305     561 
   Size:        305     256
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     561 
   Size:        405     156
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54      94      95     258     473     561 
   Size:         54      40       1     163     215      88

  Fragments arranged by size: 

                 215     163      88      54      40       1
 
HaeII r_GCGC'y

Cuts at:      0      21     180     561 
   Size:         21     159     381

  Fragments arranged by size: 

                 381     159      21
 
MscI TGG'CCA

Cuts at:      0     322     561 
   Size:        322     239
 
MslI CAynn'nnrTG

Cuts at:      0     202     561 
   Size:        202     359
 
MspA1I CmG'CkG

Cuts at:      0      51     480     492     561 
   Size:         51     429      12      69

  Fragments arranged by size: 

                 429      69      51      12
 
NcoI C'CATG_G

Cuts at:      0     323     561 
   Size:        323     238
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     329     561 
   Size:        329     232
 
PfoI T'CCnGG_A

Cuts at:      0     100     561 
   Size:        100     461
 
PpuMI rG'GwC_Cy

Cuts at:      0     258     561 
   Size:        258     303
 
PspOMI G'GGCC_C

Cuts at:      0      53      60      94     473     561 
   Size:         53       7      34     379      88

  Fragments arranged by size: 

                 379      88      53      34       7
 
PstI C_TGCA'G

Cuts at:      0     224     561 
   Size:        224     337
 
PvuII CAG'CTG

Cuts at:      0     492     561 
   Size:        492      69
 
SacI G_AGCT'C

Cuts at:      0     307     561 
   Size:        307     254
 
SacII CC_GC'GG

Cuts at:      0      52     561 
   Size:         52     509
 
SfcI C'TryA_G

Cuts at:      0     220     561 
   Size:        220     341
 
SmaI CCC'GGG

Cuts at:      0      59     109     561 
   Size:         59      50     452

  Fragments arranged by size: 

                 452      59      50
 
SmlI C'TyrA_G

Cuts at:      0     357     561 
   Size:        357     204
 
StyI C'CwwG_G

Cuts at:      0     323     561 
   Size:        323     238
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     462     561 
   Size:        462      99
 
XmaI C'CCGG_G

Cuts at:      0      57     107     561 
   Size:         57      50     454

  Fragments arranged by size: 

                 454      57      50
 
XmnI GAAnn'nnTTC

Cuts at:      0     274     561 
   Size:        274     287
 

 Enzymes that do cut:

     AcuI     AfeI     AleI    AlwNI     ApaI     AvaI     BanI    BanII
    BbvCI     BglI Bme1580I    BmgBI   Bpu10I    BpuEI    BseYI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BstYI     BtgI     BtsI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    HaeII     MscI     MslI   MspA1I
     NcoI    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI
    SacII     SfcI     SmaI     SmlI     StyI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI     BmrI     BmtI     BplI     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     ZraI
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