(Linear) (Six-base) MAPSORT of: KIBB6824.seq  Check: 3208  from: 1  to: 561



KIBB6824.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AflIII A'CryG_T

Cuts at:      0     278     561 
   Size:        278     283
 
AvaI C'yCGr_G

Cuts at:      0     308     561 
   Size:        308     253
 
BanII G_rGCy'C

Cuts at:      0     148     194     317     561 
   Size:        148      46     123     244

  Fragments arranged by size: 

                 244     148     123      46
 
BbvCI CC'TCA_GC

Cuts at:      0     538     561 
   Size:        538      23
 
BglII A'GATC_T

Cuts at:      0     338     561 
   Size:        338     223
 
Bme1580I G_kGCm'C

Cuts at:      0     164     561 
   Size:        164     397
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     561 
   Size:        403     158
 
Bpu10I CC'TnA_GC

Cuts at:      0     538     561 
   Size:        538      23
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     301     561 
   Size:         67     234     260

  Fragments arranged by size: 

                 260     234      67
 
BsaAI yAC'GTr

Cuts at:      0     159     561 
   Size:        159     402
 
BsaWI w'CCGG_w

Cuts at:      0     554     561 
   Size:        554       7
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     439     442     475     561 
   Size:        439       3      33      86

  Fragments arranged by size: 

                 439      86      33       3
 
BseYI C'CCAG_C

Cuts at:      0     489     561 
   Size:        489      72
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     561 
   Size:         39     522
 
BsiHKAI G_wGCw'C

Cuts at:      0     194     293     317     561 
   Size:        194      99      24     244

  Fragments arranged by size: 

                 244     194      99      24
 
BsmI GAATG_Cn'

Cuts at:      0      90     561 
   Size:         90     471
 
Bsp1286I G_dGCh'C

Cuts at:      0     148     164     194     293     317     561 
   Size:        148      16      30      99      24     244

  Fragments arranged by size: 

                 244     148      99      30      24      16
 
BspEI T'CCGG_A

Cuts at:      0     554     561 
   Size:        554       7
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     328     561 
   Size:        328     233
 
BstYI r'GATC_y

Cuts at:      0     338     561 
   Size:        338     223
 
EarI CTCTTCn'nnn_

Cuts at:      0      23     136     561 
   Size:         23     113     425

  Fragments arranged by size: 

                 425     113      23
 
EcoICRI GAG'CTC

Cuts at:      0     192     315     561 
   Size:        192     123     246

  Fragments arranged by size: 

                 246     192     123
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     561 
   Size:        403     158
 
EcoO109I rG'GnC_Cy

Cuts at:      0     378     525     561 
   Size:        378     147      36

  Fragments arranged by size: 

                 378     147      36
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     510     561 
   Size:        510      51
 
MslI CAynn'nnrTG

Cuts at:      0     181     561 
   Size:        181     380
 
NruI TCG'CGA

Cuts at:      0      96     561 
   Size:         96     465
 
NspI r_CATG'y

Cuts at:      0     282     561 
   Size:        282     279
 
PciI A'CATG_T

Cuts at:      0     278     561 
   Size:        278     283
 
PfoI T'CCnGG_A

Cuts at:      0     194     561 
   Size:        194     367
 
PmlI CAC'GTG

Cuts at:      0     159     561 
   Size:        159     402
 
PpuMI rG'GwC_Cy

Cuts at:      0     378     561 
   Size:        378     183
 
SacI G_AGCT'C

Cuts at:      0     194     317     561 
   Size:        194     123     244

  Fragments arranged by size: 

                 244     194     123
 
SapI GCTCTTCn'nnn_

Cuts at:      0      23     136     561 
   Size:         23     113     425

  Fragments arranged by size: 

                 425     113      23
 
SfcI C'TryA_G

Cuts at:      0     135     561 
   Size:        135     426
 
SmlI C'TyrA_G

Cuts at:      0      82     316     561 
   Size:         82     234     245

  Fragments arranged by size: 

                 245     234      82
 
StyI C'CwwG_G

Cuts at:      0     521     561 
   Size:        521      40
 
Tth111I GACn'n_nGTC

Cuts at:      0     451     561 
   Size:        451     110
 
XmnI GAAnn'nnTTC

Cuts at:      0     205     561 
   Size:        205     356
 

 Enzymes that do cut:

   AflIII     AvaI    BanII    BbvCI    BglII Bme1580I     BpmI   Bpu10I
    BpuEI    BsaAI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI   BstAPI    BstYI     EarI  EcoICRI  Eco57MI EcoO109I
     FalI     MslI     NruI     NspI     PciI     PfoI     PmlI    PpuMI
     SacI     SapI     SfcI     SmlI     StyI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaXI
    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NsiI     PacI     PasI    PflMI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
     XbaI     XcmI     XhoI     XmaI     ZraI