Restriction map for pF1KB6809
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       BamHI       BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BpmI        BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     MaeII       MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          316  GTCTAC               GT^[AC][GT]AC
AflIII        323  ACATGT               A^C[GA][CT]GT
Alw44I        208  GTGCAC               G^TGCAC
AlwI          430  GATCC                GGATC(4/5)
ApaLI         208  GTGCAC               G^TGCAC
AvaI          467  CCCGAG               C^[CT]CG[GA]G
AvrII          36  CCTAGG               C^CTAGG
BanI          420  GGCGCC               G^G[CT][GA]CC
BanII         346  GAGCTC               G[GA]GC[CT]^C
BbeI          420  GGCGCC               GGCGC^C
BbsI          375  GTCTTC               GAAGAC(2/6)
BbvCI         279  CCTCAGC              CCTCAGC(-5/-2)
BcnI          330  CCGGG                CC^[GC]GG
BfaI           37  CTAG                 C^TAG
BglI          254  GCCGCTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI           36  CCTAGG               C^CTAGG
BlpI          136  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      136  GCTCAGC              GC^T[ACGT]AGC
BpuAI         375  GTCTTC               GAAGAC(2/6)
BsoBI         467  CCCGAG               C^[CT]CG[GA]G
BspLU11I      323  ACATGT               A^CATGT
BsrBI         298  CCGCTC               CCGCTC(-3/-3)
BstXI         191  CCATCTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         429  AGATCC               [GA]^GATC[CT]
CelII         136  GCTCAGC              GC^T[ACGT]AGC
DpnII         430  GATC                 ^GATC
DsaI          229  CCGCGG               C^C[GA][CT]GG
HaeII         420  GGCGCC               [GA]GCGC^[CT]
HapII         330  CCGG                 C^CGG
HgaI          154  GACGC                GACGC(5/10)
HpaII         330  CCGG                 C^CGG
KasI          420  GGCGCC               G^GCGCC
KspI          229  CCGCGG               CCGC^GG
MaeI           37  CTAG                 C^TAG
MaeIII         41  GTCAC                ^GT[ACGT]AC
MboI          430  GATC                 ^GATC
MflI          429  AGATCC               [GA]^GATC[CT]
MslI          159  CATAAGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          330  CCGG                 C^CGG
NarI          420  GGCGCC               GG^CGCC
NciI          330  CCGGG                CC^[GC]GG
NdeII         430  GATC                 ^GATC
SacI          346  GAGCTC               GAGCT^C
SacII         229  CCGCGG               CCGC^GG
Sau3AI        430  GATC                 ^GATC
SfoI          420  GGCGCC               GGC^GCC
SmlI          292  CTCAAG               C^T[CT][GA]AG
TaqI          152  TCGA                 T^CGA
TfiI           30  GATTC                G^A[AT]TC
Tsp45I         41  GTCAC                ^GT[GC]AC
TthHB8I       152  TCGA                 T^CGA
XhoII         429  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII         230  CGCG                 CG^CG
              337  CGCG                
AcyI          154  GACGCC               G[GA]^CG[CT]C
              420  GGCGCC              
AfaI          327  GTAC                 GT^AC
              474  GTAC                
AspHI         208  GTGCAC               G[AT]GC[AT]^C
              346  GAGCTC              
AvaII         354  GGTCC                G^G[AT]CC
              384  GGACC               
BalI          185  TGGCCA               TGG^CCA
              272  TGGCCA              
BmyI          208  GTGCAC               G[AGT]GC[ACT]^C
              346  GAGCTC              
BsaHI         154  GACGCC               G[GA]^CG[CT]C
              420  GGCGCC              
BsiHKAI       208  GTGCAC               G[AT]GC[AT]^C
              346  GAGCTC              
BsiYI          86  CCTATGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              111  CCTGGCCTTGG         
BslI           86  CCTATGCCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              111  CCTGGCCTTGG         
BsmFI         143  GGGAC                GGGAC(10/14)
              442  GTCCC               
Bsp1286I      208  GTGCAC               G[AGT]GC[ACT]^C
              346  GAGCTC              
BsrI           44  ACTGG                ACTGG(1/-1)
              361  ACTGG               
BstF5I        108  CATCC                GGATG(2/0)
              276  CATCC               
BstUI         230  CGCG                 CG^CG
              337  CGCG                
CfoI          336  GCGC                 GCG^C
              421  GCGC                
EaeI          185  TGGCCA               [CT]^GGCC[GA]
              272  TGGCCA              
FokI          108  CATCC                GGATG(9/13)
              276  CATCC               
HhaI          336  GCGC                 GCG^C
              421  GCGC                
Hin1I         154  GACGCC               G[GA]^CG[CT]C
              420  GGCGCC              
HinP1I        336  GCGC                 G^CGC
              421  GCGC                
MluNI         185  TGGCCA               TGG^CCA
              272  TGGCCA              
MscI          185  TGGCCA               TGG^CCA
              272  TGGCCA              
MspA1I        139  CAGCGG               C[AC]G^C[GT]G
              229  CCGCGG              
MvnI          230  CGCG                 CG^CG
              337  CGCG                
NlaIV          82  GGCTCC               GG[ACGT]^[ACGT]CC
              420  GGCGCC              
NspI          164  GCATGT               [GA]CATG^[CT]
              323  ACATGT              
RsaI          327  GTAC                 GT^AC
              474  GTAC                
SfaNI         463  GATGC                GCATC(5/9)
              218  GCATC               
TspRI          43  CACTG                CA[GC]TG[ACGT][ACGT]^
              360  CACTG               
XcmI          107  CCATCCTGGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              188  CCACCATCTTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BssKI       
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
EcoT14I     FauI        Fnu4HI      HaeIII      HinfI       
HphI        ItaI        MboII       MnlI        MvaI        
MwoI        NlaIII      PleI        Sau96I      ScrFI       
StyI        TseI        
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