Restriction map for pF1KB6840
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DdeI        DpnII       
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MvnI        MwoI        NarI        NdeI        NdeII       
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
Sau3AI      SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI          376  AAATTC               [GA]^AATT[CT]
AflIII        239  ACGTGT               A^C[GA][CT]GT
AgeI          505  ACCGGT               A^CCGGT
AlwNI         544  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          457  GGGCCC               GGGCC^C
ApoI          376  AAATTC               [GA]^AATT[CT]
AspHI         306  GTGCTC               G[AT]GC[AT]^C
BanI          210  GGTGCC               G^G[CT][GA]CC
BbsI          394  GTCTTC               GAAGAC(2/6)
BpuAI         394  GTCTTC               GAAGAC(2/6)
BsaI          169  GAGACC               GGTCTC(1/5)
BsaWI         505  ACCGGT               [AT]^CCGG[AT]
BsiHKAI       306  GTGCTC               G[AT]GC[AT]^C
BssSI          41  CACGAG               CACGAG(-5/-1)
BstXI         223  CCATGCCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         250  GCCGGC               GC[ACGT]^[ACGT]GC
CfoI          447  GCGC                 GCG^C
FauI           30  GCGGG                CCCGC(4/6)
HaeII         446  AGCGCC               [GA]GCGC^[CT]
HgaI          434  GCGTC                GACGC(5/10)
HhaI          447  GCGC                 GCG^C
HinP1I        447  GCGC                 G^CGC
HincII        235  GTCAAC               GT[CT]^[GA]AC
HindII        235  GTCAAC               GT[CT]^[GA]AC
HinfI         148  GAATC                G^A[ACGT]TC
MspA1I        245  CAGCTG               C[AC]G^C[GT]G
NaeI          250  GCCGGC               GCC^GGC
NgoMIV        250  GCCGGC               G^CCGGC
PinAI         505  ACCGGT               A^CCGGT
PvuII         245  CAGCTG               CAG^CTG
SapI          534  GAAGAGC              GCTCTTC(1/4)
SmaI          347  CCCGGG               CCC^GGG
TfiI          148  GAATC                G^A[AT]TC
Tsp45I        103  GTCAC                ^GT[GC]AC
TspRI         304  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmaCI         347  CCCGGG               C^CCGGG
XmaI          347  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BanII          44  GAGCCC               G[GA]GC[CT]^C
              457  GGGCCC              
BcgI           43  CGAGCCCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              107  CGACTGTGCTGC        
BcnI          347  CCCGG                CC^[GC]GG
              348  CCGGG               
BsmAI          37  GAGAC                GTCTC(1/5)
              169  GAGAC               
BsmFI         182  GGGAC                GGGAC(10/14)
              290  GGGAC               
BspMI         254  GCAGGT               ACCTGC(4/8)
              390  GCAGGT              
BsrFI         250  GCCGGC               [GA]^CCGG[CT]
              505  ACCGGT              
BsrI          216  CCAGT                ACTGG(1/-1)
              543  CCAGT               
Cfr10I        250  GCCGGC               [GA]^CCGG[CT]
              505  ACCGGT              
DraII         457  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              464  AGGCCCT             
DsaI           65  CCATGG               C^C[GA][CT]GG
              527  CCATGG              
EcoO109I      457  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              464  AGGCCCT             
EcoT14I        65  CCATGG               C^C[AT][AT]GG
              527  CCATGG              
MaeII         239  ACGT                 A^CGT
              356  ACGT                
MaeIII         61  GTAAC                ^GT[ACGT]AC
              103  GTCAC               
NciI          347  CCCGG                CC^[GC]GG
              348  CCGGG               
NcoI           65  CCATGG               C^CATGG
              527  CCATGG              
StyI           65  CCATGG               C^C[AT][AT]GG
              527  CCATGG              
Tsp509I       334  AATT                 ^AATT
              377  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        AvaII       
BbvI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsoBI       Bsp1286I    BssKI       BstF5I      
BstNI       Cfr13I      EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HpaII       HphI        
ItaI        Ksp632I     MboII       MnlI        MslI        
MspI        MvaI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com