(Circular) (Six-base) MAPSORT of: pF1KB6840.seq Check: 4595 from: 1 to: 3686 pF1KB6840 3686 bp With 182 enzymes: SgfI * April 24, 2009 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 353 353 Size: 3686 AccI GT'mk_AC Cuts at: 724 724 Size: 3686 Acc65I G'GTAC_C Cuts at: 702 702 Size: 3686 AclI AA'CG_TT Cuts at: 3526 3526 Size: 3686 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 325 1511 1943 2690 2958 325 Size: 1186 432 747 268 1053 Fragments arranged by size: 1186 1053 747 432 268 AflIII A'CryG_T Cuts at: 347 1038 2142 3119 347 Size: 691 1104 977 914 Fragments arranged by size: 1104 977 914 691 AgeI A'CCGG_T Cuts at: 613 2074 613 Size: 1461 2225 AleI CACnn'nnGTG Cuts at: 217 217 Size: 3686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 334 334 Size: 3686 AlwNI CAG_nnn'CTG Cuts at: 657 991 2558 657 Size: 334 1567 1785 Fragments arranged by size: 1785 1567 334 ApaI G_GGCC'C Cuts at: 569 569 Size: 3686 ApaLI G'TGCA_C Cuts at: 2456 2456 Size: 3686 ApoI r'AATT_y Cuts at: 484 690 3039 3295 484 Size: 206 2349 256 875 Fragments arranged by size: 2349 875 256 206 AseI AT'TA_AT Cuts at: 20 20 Size: 3686 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3686 AvaI C'yCGr_G Cuts at: 395 455 524 706 395 Size: 60 69 182 3375 Fragments arranged by size: 3375 182 69 60 BamHI G'GATC_C Cuts at: 711 711 Size: 3686 BanI G'GyrC_C Cuts at: 318 702 1116 1364 1399 318 Size: 384 414 248 35 2605 Fragments arranged by size: 2605 414 384 248 35 BanII G_rGCy'C Cuts at: 156 569 700 1730 156 Size: 413 131 1030 2112 Fragments arranged by size: 2112 1030 413 131 BbeI G_GCGC'C Cuts at: 1120 1368 1120 Size: 248 3438 BbsI GAAGACnn'nnnn_ Cuts at: 495 495 Size: 3686 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 140 174 204 238 140 Size: 34 30 34 3588 Fragments arranged by size: 3588 34 34 30 BciVI GTATCCnnnnn_n' Cuts at: 1584 2345 3686 1584 Size: 761 1341 1584 Fragments arranged by size: 1584 1341 761 BclI T'GATC_A Cuts at: 1206 1206 Size: 3686 BglII A'GATC_T Cuts at: 1201 3238 1201 Size: 2037 1649 BlpI GC'TnA_GC Cuts at: 798 798 Size: 3686 Bme1580I G_kGCm'C Cuts at: 323 569 1311 1404 2460 323 Size: 246 742 93 1056 1549 Fragments arranged by size: 1549 1056 742 246 93 BmgBI CAC'GTC Cuts at: 465 465 Size: 3686 BmrI ACTGGGnnnn_n' Cuts at: 318 898 979 1314 3458 318 Size: 580 81 335 2144 546 Fragments arranged by size: 2144 580 546 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2053 2053 Size: 3686 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 862 1741 2233 2531 862 Size: 879 492 298 2017 Fragments arranged by size: 2017 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 271 3390 271 Size: 3119 567 BsaAI yAC'GTr Cuts at: 1039 1039 Size: 3686 BsaBI GATnn'nnATC Cuts at: 6 1200 3237 6 Size: 1194 2037 455 Fragments arranged by size: 2037 1194 455 BsaHI Gr'CG_yC Cuts at: 1117 1365 2067 3563 1117 Size: 248 702 1496 1240 Fragments arranged by size: 1496 1240 702 248 BsaWI w'CCGG_w Cuts at: 613 875 1396 2074 2348 2495 2906 613 Size: 262 521 678 274 147 411 1393 Fragments arranged by size: 1393 678 521 411 274 262 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 499 529 499 Size: 30 3656 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 313 568 613 313 Size: 255 45 3386 Fragments arranged by size: 3386 255 45 BseYI C'CCAG_C Cuts at: 1111 1869 2446 2942 1111 Size: 758 577 496 1855 Fragments arranged by size: 1855 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1228 1274 2482 104 Size: 1124 46 1208 1308 Fragments arranged by size: 1308 1208 1124 46 BsiHKAI G_wGCw'C Cuts at: 418 700 1478 1668 2460 418 Size: 282 778 190 792 1644 Fragments arranged by size: 1644 792 778 282 190 Bsp1286I G_dGCh'C Cuts at: 156 323 418 569 700 1311 1404 1478 1668 Size: 167 95 151 131 611 93 74 190 Cuts at: 1668 1730 2460 156 Size: 62 730 1382 Fragments arranged by size: 1382 730 611 190 167 151 131 95 93 74 62 BspEI T'CCGG_A Cuts at: 2906 2906 Size: 3686 BspHI T'CATG_A Cuts at: 2862 2862 Size: 3686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 353 489 736 950 1252 1633 353 Size: 136 247 214 302 381 2406 Fragments arranged by size: 2406 381 302 247 214 136 BsrBI CCG'CTC Cuts at: 50 1978 2032 3516 3681 50 Size: 1928 54 1484 165 55 Fragments arranged by size: 1928 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1598 1598 Size: 3686 BsrFI r'CCGG_y Cuts at: 358 613 1684 1865 2074 358 Size: 255 1071 181 209 1970 Fragments arranged by size: 1970 1071 255 209 181 BssSI C'ACGA_G Cuts at: 149 1957 2315 149 Size: 1808 358 1520 Fragments arranged by size: 1808 1520 358 BstAPI GCAn_nnn'nTGC Cuts at: 1063 1063 Size: 3686 BstBI TT'CG_AA Cuts at: 2047 2047 Size: 3686 BstXI CCAn_nnnn'nTGG Cuts at: 338 338 Size: 3686 BstYI r'GATC_y Cuts at: 1 711 1178 1201 1536 1782 2794 2880 2892 Size: 710 467 23 335 246 1012 86 12 Cuts at: 2892 3238 1 Size: 346 449 Fragments arranged by size: 1012 710 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 173 635 107 Size: 66 462 3158 Fragments arranged by size: 3158 462 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1636 1817 1980 1636 Size: 181 163 3342 Fragments arranged by size: 3342 181 163 BtsI GCAGTG_nn' Cuts at: 418 929 418 Size: 511 3175 DraI TTT'AAA Cuts at: 685 685 Size: 3686 DrdI GACnn_nn'nnGTC Cuts at: 1392 2250 1392 Size: 858 2828 EaeI y'GGCC_r Cuts at: 1271 1445 1836 1863 3208 1271 Size: 174 391 27 1345 1749 Fragments arranged by size: 1749 1345 391 174 27 EagI C'GGCC_G Cuts at: 1271 1271 Size: 3686 EarI CTCTTCn'nnn_ Cuts at: 586 595 637 1709 1919 586 Size: 9 42 1072 210 2353 Fragments arranged by size: 2353 1072 210 42 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1894 2204 2350 3496 1894 Size: 310 146 1146 2084 Fragments arranged by size: 2084 1146 310 146 EcoICRI GAG'CTC Cuts at: 698 698 Size: 3686 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 325 1511 1943 2690 2958 325 Size: 1186 432 747 268 1053 Fragments arranged by size: 1186 1053 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 566 573 825 566 Size: 7 252 3427 Fragments arranged by size: 3427 252 7 EcoRI G'AATT_C Cuts at: 690 690 Size: 3686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1705 1705 Size: 3686 FspI TGC'GCA Cuts at: 1467 1467 Size: 3686 HaeII r_GCGC'y Cuts at: 558 1120 1368 2390 3369 558 Size: 562 248 1022 979 875 Fragments arranged by size: 1022 979 875 562 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1528 1558 1560 1590 1528 Size: 30 2 30 3624 Fragments arranged by size: 3624 30 30 2 HincII GTy'rAC Cuts at: 345 725 345 Size: 380 3306 KasI G'GCGC_C Cuts at: 1116 1364 1116 Size: 248 3438 KpnI G_GTAC'C Cuts at: 706 706 Size: 3686 MluI A'CGCG_T Cuts at: 3119 3119 Size: 3686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2357 2541 2357 Size: 184 3502 MscI TGG'CCA Cuts at: 1447 1447 Size: 3686 MslI CAynn'nnrTG Cuts at: 178 217 336 1675 1802 178 Size: 39 119 1339 127 2062 Fragments arranged by size: 2062 1339 127 119 39 MspA1I CmG'CkG Cuts at: 355 797 1111 1471 2484 2729 355 Size: 442 314 360 1013 245 1312 Fragments arranged by size: 1312 1013 442 360 314 245 NaeI GCC'GGC Cuts at: 360 1867 360 Size: 1507 2179 NarI GG'CG_CC Cuts at: 1117 1365 1117 Size: 248 3438 NcoI C'CATG_G Cuts at: 107 173 635 107 Size: 66 462 3158 Fragments arranged by size: 3158 462 66 NgoMIV G'CCGG_C Cuts at: 358 1865 358 Size: 1507 2179 NspI r_CATG'y Cuts at: 739 1239 2146 739 Size: 500 907 2279 Fragments arranged by size: 2279 907 500 PciI A'CATG_T Cuts at: 2142 2142 Size: 3686 PmeI GTTT'AAAC Cuts at: 685 685 Size: 3686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 2857 2825 Size: 32 3654 PspOMI G'GGCC_C Cuts at: 565 565 Size: 3686 PstI C_TGCA'G Cuts at: 733 1418 733 Size: 685 3001 PvuI CG_AT'CG Cuts at: 104 104 Size: 3686 PvuII CAG'CTG Cuts at: 355 1111 1471 355 Size: 756 360 2570 Fragments arranged by size: 2570 756 360 RsrII CG'GwC_CG Cuts at: 1881 1881 Size: 3686 SacI G_AGCT'C Cuts at: 700 700 Size: 3686 SalI G'TCGA_C Cuts at: 723 723 Size: 3686 SapI GCTCTTCn'nnn_ Cuts at: 637 1709 1919 637 Size: 1072 210 2404 Fragments arranged by size: 2404 1072 210 SbfI CC_TGCA'GG Cuts at: 733 733 Size: 3686 ScaI AGT'ACT Cuts at: 3203 3203 Size: 3686 SfcI C'TryA_G Cuts at: 33 729 1414 2407 2598 33 Size: 696 685 993 191 1121 Fragments arranged by size: 1121 993 696 685 191 SfoI GGC'GCC Cuts at: 1118 1366 1118 Size: 248 3438 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3686 SmaI CCC'GGG Cuts at: 457 708 457 Size: 251 3435 SmlI C'TyrA_G Cuts at: 841 1756 2248 2510 841 Size: 915 492 262 2017 Fragments arranged by size: 2017 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3686 SphI G_CATG'C Cuts at: 739 1239 739 Size: 500 3186 SrfI GCCC'GGGC Cuts at: 457 457 Size: 3686 StyI C'CwwG_G Cuts at: 107 173 635 820 1174 107 Size: 66 462 185 354 2619 Fragments arranged by size: 2619 462 354 185 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 154 1269 1402 1861 2070 154 Size: 1115 133 459 209 1770 Fragments arranged by size: 1770 1115 459 209 133 TatI w'GTAC_w Cuts at: 3201 3201 Size: 3686 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 247 279 3203 3235 247 Size: 32 2924 32 698 Fragments arranged by size: 2924 698 32 32 Tth111I GACn'n_nGTC Cuts at: 1483 1483 Size: 3686 XbaI T'CTAG_A Cuts at: 717 717 Size: 3686 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1181 1181 Size: 3686 XmaI C'CCGG_G Cuts at: 455 706 455 Size: 251 3435 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AloI AscI AvrII BaeI BbvCI BfrBI BglI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI XmnI ZraI