Restriction map for pF1KB6802
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmFI       BsmI        BsoBI       BspDI       
BspEI       BspLU11I    BspMI       BsrDI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DraI        DraII       DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MamI        MluI        
MluNI       MroI        MscI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StyI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AlwNI         442  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         426  GGTCC                G^G[AT]CC
BanII         468  GAGCCC               G[GA]GC[CT]^C
BbsI          545  GAAGAC               GAAGAC(2/6)
BmyI          468  GAGCCC               G[AGT]GC[ACT]^C
BpuAI         545  GAAGAC               GAAGAC(2/6)
BsaI          273  GGTCTC               GGTCTC(1/5)
BsgI          343  CTGCAC               GTGCAG(16/14)
BsmBI          62  GAGACG               CGTCTC(1/5)
Bsp1286I      468  GAGCCC               G[AGT]GC[ACT]^C
BspHI         308  TCATGA               T^CATGA
BsrBI         509  CCGCTC               CCGCTC(-3/-3)
BsrFI         230  GCCGGC               [GA]^CCGG[CT]
BsrI          291  ACTGG                ACTGG(1/-1)
BssSI         223  CACGAG               CACGAG(-5/-1)
BstAPI        345  GCACACCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        230  GCCGGC               [GA]^CCGG[CT]
DdeI          541  CTCAG                C^T[ACGT]AG
DraIII        346  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          237  CCGCGG               C^C[GA][CT]GG
EcoRI         518  GAATTC               G^AATTC
HgaI           64  GACGC                GACGC(5/10)
HinfI         169  GATTC                G^A[ACGT]TC
KspI          237  CCGCGG               CCGC^GG
MaeII         383  ACGT                 A^CGT
MfeI           73  CAATTG               C^AATTG
MslI          112  CACGATAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI           73  CAATTG               C^AATTG
NaeI          230  GCCGGC               GCC^GGC
NgoMIV        230  GCCGGC               G^CCGGC
NlaIV         233  GGCTCC               GG[ACGT]^[ACGT]CC
RcaI          308  TCATGA               T^CATGA
SacII         237  CCGCGG               CCGC^GG
StuI          124  AGGCCT               AGG^CCT
TfiI          169  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII          66  CGCG                 CG^CG
              238  CGCG                
AcsI          214  AAATTC               [GA]^AATT[CT]
              518  GAATTC              
AfaI           36  GTAC                 GT^AC
              256  GTAC                
AluI          247  AGCT                 AG^CT
              401  AGCT                
AlwI           21  GATCC                GGATC(4/5)
              365  GATCC               
ApoI          214  AAATTC               [GA]^AATT[CT]
              518  GAATTC              
BbvI          163  GCAGC                GCAGC(8/12)
              248  GCTGC               
BpmI          276  CTCCAG               CTGGAG(16/14)
              403  CTCCAG              
BsaJI         237  CCGCGG               C^C[ACGT][ACGT]GG
              269  CCGGGG              
Bsp1407I       35  TGTACA               T^GTACA
              255  TGTACA              
BsrGI          35  TGTACA               T^GTACA
              255  TGTACA              
BstUI          66  CGCG                 CG^CG
              238  CGCG                
BstYI          20  AGATCC               [GA]^GATC[CT]
              364  AGATCC              
Cac8I         230  GCCGGC               GC[ACGT]^[ACGT]GC
              354  GCGTGC              
Cfr13I        426  GGTCC                G^G[ACGT]CC
              533  GGGCC               
EaeI          266  TGGCCG               [CT]^GGCC[GA]
              506  TGGCCG              
MflI           20  AGATCC               [GA]^GATC[CT]
              364  AGATCC              
MseI          143  TTAA                 T^TAA
              212  TTAA                
MspA1I        164  CAGCGG               C[AC]G^C[GT]G
              237  CCGCGG              
MvnI           66  CGCG                 CG^CG
              238  CGCG                
NlaIII        309  CATG                 CATG^
              421  CATG                
RsaI           36  GTAC                 GT^AC
              256  GTAC                
Sau96I        426  GGTCC                G^G[ACGT]CC
              533  GGGCC               
SspBI          35  TGTACA               T^GTACA
              255  TGTACA              
Tru9I         143  TTAA                 T^TAA
              212  TTAA                
TseI          163  GCAGC                G^C[AT]GC
              248  GCTGC               
XhoII          20  AGATCC               [GA]^GATC[CT]
              364  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        BcnI        BsiYI       BslI        BsmAI       
BssKI       BstF5I      BstNI       DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       Sau3AI      ScrFI       SfaNI       Tsp45I      
Tsp509I     TspRI       XcmI        
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