Restriction map for pF1KB6802
 (Linear) (Six-base) MAPSORT of: KIBB6802.seq  Check: 4053  from: 1  to: 552



KIBB6802.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     544     552 
   Size:        469      75       8

  Fragments arranged by size: 

                 469      75       8
 
AleI CACnn'nnGTG

Cuts at:      0     116     552 
   Size:        116     436
 
AlwNI CAG_nnn'CTG

Cuts at:      0     447     552 
   Size:        447     105
 
ApoI r'AATT_y

Cuts at:      0     214     518     552 
   Size:        214     304      34

  Fragments arranged by size: 

                 304     214      34
 
BanII G_rGCy'C

Cuts at:      0     472     552 
   Size:        472      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     552 
   Size:        552
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     261     388     552 
   Size:        261     127     164

  Fragments arranged by size: 

                 261     164     127
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     279     552 
   Size:        279     273
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     354     384     552 
   Size:        354      30     168

  Fragments arranged by size: 

                 354     168      30
 
BseYI C'CCAG_C

Cuts at:      0     159     329     358     552 
   Size:        159     170      29     194

  Fragments arranged by size: 

                 194     170     159      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     552 
   Size:        328     224
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      56     552 
   Size:         56     496
 
Bsp1286I G_dGCh'C

Cuts at:      0     472     552 
   Size:        472      80
 
BspHI T'CATG_A

Cuts at:      0     308     552 
   Size:        308     244
 
BsrBI CCG'CTC

Cuts at:      0     511     552 
   Size:        511      41
 
BsrFI r'CCGG_y

Cuts at:      0     230     552 
   Size:        230     322
 
BsrGI T'GTAC_A

Cuts at:      0      35     255     552 
   Size:         35     220     297

  Fragments arranged by size: 

                 297     220      35
 
BssSI C'ACGA_G

Cuts at:      0     223     552 
   Size:        223     329
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     351     552 
   Size:        351     201
 
BstYI r'GATC_y

Cuts at:      0      20     364     552 
   Size:         20     344     188

  Fragments arranged by size: 

                 344     188      20
 
BtgI C'CryG_G

Cuts at:      0     237     552 
   Size:        237     315
 
BtsI GCAGTG_nn'

Cuts at:      0     258     448     552 
   Size:        258     190     104

  Fragments arranged by size: 

                 258     190     104
 
DraIII CAC_nnn'GTG

Cuts at:      0     351     552 
   Size:        351     201
 
EaeI y'GGCC_r

Cuts at:      0     266     506     552 
   Size:        266     240      46

  Fragments arranged by size: 

                 266     240      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     261     388     469     544     552 
   Size:        261     127      81      75       8

  Fragments arranged by size: 

                 261     127      81      75       8
 
EcoRI G'AATT_C

Cuts at:      0     518     552 
   Size:        518      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     552 
   Size:        160      32     360

  Fragments arranged by size: 

                 360     160      32
 
MfeI C'AATT_G

Cuts at:      0      73     552 
   Size:         73     479
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     552 
   Size:        113     439
 
MslI CAynn'nnrTG

Cuts at:      0     116     552 
   Size:        116     436
 
MspA1I CmG'CkG

Cuts at:      0     166     239     552 
   Size:        166      73     313

  Fragments arranged by size: 

                 313     166      73
 
NaeI GCC'GGC

Cuts at:      0     232     552 
   Size:        232     320
 
NgoMIV G'CCGG_C

Cuts at:      0     230     552 
   Size:        230     322
 
PfoI T'CCnGG_A

Cuts at:      0     277     404     513     552 
   Size:        277     127     109      39

  Fragments arranged by size: 

                 277     127     109      39
 
SacII CC_GC'GG

Cuts at:      0     240     552 
   Size:        240     312
 
StuI AGG'CCT

Cuts at:      0     126     552 
   Size:        126     426
 
TatI w'GTAC_w

Cuts at:      0      35     255     552 
   Size:         35     220     297

  Fragments arranged by size: 

                 297     220      35
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     354     386     552 
   Size:        354      32     166

  Fragments arranged by size: 

                 354     166      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     129     381     501     552 
   Size:        129     252     120      51

  Fragments arranged by size: 

                 252     129     120      51
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI    BanII     BbsI     BpmI     BsaI
    BsaXI    BseYI     BsgI    BsmBI Bsp1286I    BspHI    BsrBI    BsrFI
    BsrGI    BssSI   BstAPI    BstYI     BtgI     BtsI   DraIII     EaeI
  Eco57MI    EcoRI    Hin4I     MfeI     MmeI     MslI   MspA1I     NaeI
   NgoMIV     PfoI    SacII     StuI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI
    BspMI    BsrDI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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