Restriction map for pF1KB8232
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        Alw44I      AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspHI       AspI        AviII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstZ17I     
Bsu36I      Cac8I       CelII       ClaI        CpoI        
DraIII      DrdI        EaeI        EagI        EclXI       
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
FauI        FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AciI          763  GCGG                 CCGC(-3/-1)
AcyI            3  GGCGTC               G[GA]^CG[CT]C
AflIII         40  ACATGT               A^C[GA][CT]GT
AhdI          637  GACTTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       600  AGCGCT               AGC^GCT
AspEI         637  GACTTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         806  GGACC                G^G[AT]CC
AvrII         520  CCTAGG               C^CTAGG
BbsI          668  GTCTTC               GAAGAC(2/6)
BcnI          129  CCGGG                CC^[GC]GG
BglI          781  GCCTCACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         891  AGATCT               A^GATCT
BlnI          520  CCTAGG               C^CTAGG
BpuAI         668  GTCTTC               GAAGAC(2/6)
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
BseRI         741  GAGGAG               GAGGAG(10/8)
BsiYI         103  CCTGAATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          103  CCTGAATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         488  GTCTC                GTCTC(1/5)
BsmFI         635  GGGAC                GGGAC(10/14)
BspLU11I       40  ACATGT               A^CATGT
BsrFI          85  GCCGGT               [GA]^CCGG[CT]
BstEII        124  GGTGACC              G^GT[ACGT]ACC
BstPI         124  GGTGACC              G^GT[ACGT]ACC
CfoI          601  GCGC                 GCG^C
Cfr10I         85  GCCGGT               [GA]^CCGG[CT]
Cfr13I        806  GGACC                G^G[ACGT]CC
DraII         805  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          368  CCATGG               C^C[GA][CT]GG
Eam1105I      637  GACTTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      600  AGCGCT               AGC^GCT
EcoO109I      805  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       124  GGTGACC              G^GT[ACGT]ACC
EcoT22I       347  ATGCAT               ATGCA^T
HaeII         600  AGCGCT               [GA]GCGC^[CT]
HaeIII        780  GGCC                 GG^CC
HgaI            4  GCGTC                GACGC(5/10)
HhaI          601  GCGC                 GCG^C
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
HinP1I        601  GCGC                 G^CGC
NciI          129  CCGGG                CC^[GC]GG
NcoI          368  CCATGG               C^CATGG
NsiI          347  ATGCAT               ATGCA^T
NspI           40  ACATGT               [GA]CATG^[CT]
PleI           35  GAGTC                GAGTC(4/5)
PpuMI         805  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII         794  CAGCTG               CAG^CTG
Sau96I        806  GGACC                G^G[ACGT]CC
XcmI          810  CCAAAAAGAAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI          300  GTAC                 GT^AC
              682  GTAC                
AlwI          187  GATCC                GGATC(4/5)
              145  GGATC               
AlwNI         263  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              648  CAGGTTCTG           
Asp700I       345  GAATGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              511  GAAGGGGTTC          
AvaI          194  CTCGAG               C^[CT]CG[GA]G
              881  CTCGAG              
BanII         744  GAGCCC               G[GA]GC[CT]^C
              884  GAGCCC              
BfaI          521  CTAG                 C^TAG
              604  CTAG                
BsmI          345  GAATGC               GAATGC(1/-1)
              349  GCATTC              
BsoBI         194  CTCGAG               C^[CT]CG[GA]G
              881  CTCGAG              
BsrDI         167  GCAATG               GCAATG(2/0)
              337  GCAATG              
BstF5I         52  CATCC                GGATG(2/0)
               21  GGATG               
BstNI         647  CCAGG                CC^[AT]GG
              736  CCTGG               
BstXI          11  CCAGCCGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              749  CCATCTCTGTGG        
DraI          529  TTTAAA               TTT^AAA
              583  TTTAAA              
EcoRII        647  CCAGG                ^CC[AT]GG
              736  CCTGG               
EcoT14I       368  CCATGG               C^C[AT][AT]GG
              520  CCTAGG              
FokI           52  CATCC                GGATG(9/13)
               21  GGATG               
HapII          86  CCGG                 C^CGG
              129  CCGG                
HpaII          86  CCGG                 C^CGG
              129  CCGG                
HphI           88  GGTGA                GGTGA(8/7)
              124  GGTGA               
MaeI          521  CTAG                 C^TAG
              604  CTAG                
MseI          530  TTAA                 T^TAA
              584  TTAA                
MspA1I        761  CAGCGG               C[AC]G^C[GT]G
              794  CAGCTG              
MspI           86  CCGG                 C^CGG
              129  CCGG                
MvaI          647  CCAGG                CC^[AT]GG
              736  CCTGG               
PaeR7I        194  CTCGAG               C^TCGAG
              881  CTCGAG              
RsaI          300  GTAC                 GT^AC
              682  GTAC                
SmlI          194  CTCGAG               C^T[CT][GA]AG
              881  CTCGAG              
StyI          368  CCATGG               C^C[AT][AT]GG
              520  CCTAGG              
Tru9I         530  TTAA                 T^TAA
              584  TTAA                
Tsp45I         37  GTCAC                ^GT[GC]AC
              125  GTGAC               
TspRI         612  CAGTG                CA[GC]TG[ACGT][ACGT]^
              926  CAGTG               
XhoI          194  CTCGAG               C^TCGAG
              881  CTCGAG              
XmnI          345  GAATGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              511  GAAGGGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AccI        AcsI        AluI        ApoI        BbvI        
BmyI        BsaJI       Bsp1286I    BsrI        BssKI       
BstYI       DdeI        DpnII       EarI        Fnu4HI      
HinfI       ItaI        Ksp632I     MaeIII      MboI        
MboII       MflI        MnlI        MslI        MwoI        
NdeII       NlaIII      NlaIV       SapI        Sau3AI      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
TseI        Tsp509I     TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com