Restriction map for pF1KB6757
##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
ItaI        KasI        KpnI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII          12  CGCG                 CG^CG
AflII         301  CTTAAG               C^TTAAG
AluI          152  AGCT                 AG^CT
AvaII         247  GGACC                G^G[AT]CC
BamHI         144  GGATCC               G^GATCC
BbsI           95  GTCTTC               GAAGAC(2/6)
BfrI          301  CTTAAG               C^TTAAG
BpuAI          95  GTCTTC               GAAGAC(2/6)
BseRI         274  CTCCTC               GAGGAG(10/8)
BsiYI         251  CCAGGCCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          251  CCAGGCCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          58  GTCCC                GGGAC(10/14)
BsrI          230  ACTGG                ACTGG(1/-1)
BssKI         251  CCAGG                ^CC[ACGT]GG
BstNI         251  CCAGG                CC^[AT]GG
BstUI          12  CGCG                 CG^CG
Cac8I          21  GCGTGC               GC[ACGT]^[ACGT]GC
Cfr13I        247  GGACC                G^G[ACGT]CC
DraII         246  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI           11  CCGCGG               C^C[GA][CT]GG
EarI          132  GAAGAG               CTCTTC(1/4)
EcoO109I      246  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRII        251  CCAGG                ^CC[AT]GG
FauI           10  CCCGC                CCCGC(4/6)
HaeIII        254  GGCC                 GG^CC
HphI           15  GGTGA                GGTGA(8/7)
Ksp632I       132  GAAGAG               CTCTTC(1/4)
KspI           11  CCGCGG               CCGC^GG
MseI          302  TTAA                 T^TAA
MspA1I         11  CCGCGG               C[AC]G^C[GT]G
MvaI          251  CCAGG                CC^[AT]GG
MvnI           12  CGCG                 CG^CG
NlaIII         63  CATG                 CATG^
PflMI         251  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         246  AGGACCC              [GA]G^G[AT]CC[CT]
SacII          11  CCGCGG               CCGC^GG
Sau96I        247  GGACC                G^G[ACGT]CC
ScrFI         251  CCAGG                CC^[ACGT]GG
SmlI          301  CTTAAG               C^T[CT][GA]AG
SspI          238  AATATT               AAT^ATT
StuI          253  AGGCCT               AGG^CCT
TaqI          243  TCGA                 T^CGA
Tru9I         302  TTAA                 T^TAA
TthHB8I       243  TCGA                 T^CGA
Van91I        251  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI           11  CCGC                 CCGC(-3/-1)
               13  GCGG                
BstYI         144  GGATCC               [GA]^GATC[CT]
              264  AGATCC              
DdeI          149  CTGAG                C^T[ACGT]AG
              308  CTAAG               
DpnII         145  GATC                 ^GATC
              265  GATC                
EcoT14I       256  CCTTGG               C^C[AT][AT]GG
              279  CCAAGG              
MboI          145  GATC                 ^GATC
              265  GATC                
MflI          144  GGATCC               [GA]^GATC[CT]
              264  AGATCC              
NdeII         145  GATC                 ^GATC
              265  GATC                
NlaIV         144  GGATCC               GG[ACGT]^[ACGT]CC
              247  GGACCC              
Sau3AI        145  GATC                 ^GATC
              265  GATC                
SfaNI          18  GATGC                GCATC(5/9)
              124  GATGC               
StyI          256  CCTTGG               C^C[AT][AT]GG
              279  CCAAGG              
XhoII         144  GGATCC               [GA]^GATC[CT]
              264  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

AlwI        BsaJI       HinfI       MboII       MnlI        
PleI        
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