Restriction map for pF1KB6569
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsiYI       BslI        BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MluI        
MroI        MseI        MslI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        TaqI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         148  CGCG                 CG^CG
AfaI          712  GTAC                 GT^AC
Alw44I        840  GTGCAC               G^TGCAC
ApaLI         840  GTGCAC               G^TGCAC
Asp700I       786  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          768  TGGCCA               TGG^CCA
BbsI           69  GAAGAC               GAAGAC(2/6)
BglII        1058  AGATCT               A^GATCT
BpuAI          69  GAAGAC               GAAGAC(2/6)
BseRI          13  CTCCTC               GAGGAG(10/8)
BsgI           20  CTGCAC               GTGCAG(16/14)
BspMI         687  GCAGGT               ACCTGC(4/8)
BstAPI        547  GCAGGGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         148  CGCG                 CG^CG
BstYI        1058  AGATCT               [GA]^GATC[CT]
DpnII        1059  GATC                 ^GATC
DsaI          563  CCATGG               C^C[GA][CT]GG
EaeI          768  TGGCCA               [CT]^GGCC[GA]
EcoT14I       563  CCATGG               C^C[AT][AT]GG
FauI         1037  GCGGG                CCCGC(4/6)
HgaI         1104  GCGTC                GACGC(5/10)
MboI         1059  GATC                 ^GATC
MflI         1058  AGATCT               [GA]^GATC[CT]
MluNI         768  TGGCCA               TGG^CCA
MscI          768  TGGCCA               TGG^CCA
MvnI          148  CGCG                 CG^CG
NcoI          563  CCATGG               C^CATGG
NdeII        1059  GATC                 ^GATC
NruI          147  TCGCGA               TCG^CGA
NspI          452  GCATGC               [GA]CATG^[CT]
PstI          212  CTGCAG               CTGCA^G
RsaI          712  GTAC                 GT^AC
SacI          754  GAGCTC               GAGCT^C
Sau3AI       1059  GATC                 ^GATC
SfaNI         228  GATGC                GCATC(5/9)
SfcI          212  CTGCAG               C^T[GA][CT]AG
SfiI          478  GGCCTCGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          600  CCCGGG               CCC^GGG
SphI          452  GCATGC               GCATG^C
StyI          563  CCATGG               C^C[AT][AT]GG
TfiI          949  GATTC                G^A[AT]TC
XhoII        1058  AGATCT               [GA]^GATC[CT]
XmaCI         600  CCCGGG               C^CCGGG
XmaI          600  CCCGGG               C^CCGGG
XmnI          786  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AocI          391  CCTCAGG              CC^T[ACGT]AGG
              490  CCTAAGG             
AspHI         754  GAGCTC               G[AT]GC[AT]^C
              840  GTGCAC              
AvaI          176  CTCGGG               C^[CT]CG[GA]G
              600  CCCGGG              
BanI          442  GGTGCC               G^G[CT][GA]CC
              586  GGTGCC              
BglI          173  GCCCTCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              479  GCCTCGCAGGC         
BsiHKAI       754  GAGCTC               G[AT]GC[AT]^C
              840  GTGCAC              
BsmAI         299  GAGAC                GTCTC(1/5)
              632  GAGAC               
BsmI          888  GAATGC               GAATGC(1/-1)
              623  GCATTC              
BsoBI         176  CTCGGG               C^[CT]CG[GA]G
              600  CCCGGG              
BstF5I        159  GGATG                GGATG(2/0)
              227  GGATG               
Bsu36I        391  CCTCAGG              CC^T[ACGT]AGG
              490  CCTAAGG             
Eco81I        391  CCTCAGG              CC^T[ACGT]AGG
              490  CCTAAGG             
FokI          159  GGATG                GGATG(9/13)
              227  GGATG               
HapII         332  CCGG                 C^CGG
              601  CCGG                
HpaII         332  CCGG                 C^CGG
              601  CCGG                
MspI          332  CCGG                 C^CGG
              601  CCGG                
PleI          524  GAGTC                GAGTC(4/5)
              988  GAGTC               
SapI            6  GCTCTTC              GCTCTTC(1/4)
              756  GCTCTTC             
StuI          477  AGGCCT               AGG^CCT
              695  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        AlwNI       ApaI        AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DdeI        DraII       
EarI        EcoO109I    EcoRII      Fnu4HI      HaeIII      
HinfI       HphI        ItaI        Ksp632I     MboII       
MnlI        MspA1I      MvaI        MwoI        NciI        
NlaIII      NlaIV       PpuMI       PvuII       Sau96I      
ScrFI       TseI        TspRI       
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