Restriction map for pF1KB6550
 (Linear) (Six-base) MAPSORT of: KIBB6550.seq  Check: 291  from: 1  to: 1176



KIBB6550.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     371     455    1172    1176 
   Size:        371      84     717       4

  Fragments arranged by size: 

                 717     371      84       4
 
AflIII A'CryG_T

Cuts at:      0     372    1176 
   Size:        372     804
 
ApoI r'AATT_y

Cuts at:      0      85     522    1176 
   Size:         85     437     654

  Fragments arranged by size: 

                 654     437      85
 
AvaI C'yCGr_G

Cuts at:      0      53     887    1176 
   Size:         53     834     289

  Fragments arranged by size: 

                 834     289      53
 
BanI G'GyrC_C

Cuts at:      0      46    1176 
   Size:         46    1130
 
BanII G_rGCy'C

Cuts at:      0    1150    1176 
   Size:       1150      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1142    1176 
   Size:       1142      34
 
BbvCI CC'TCA_GC

Cuts at:      0     772    1176 
   Size:        772     404
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     541     575    1176 
   Size:        541      34     601

  Fragments arranged by size: 

                 601     541      34
 
BclI T'GATC_A

Cuts at:      0     621    1176 
   Size:        621     555
 
Bme1580I G_kGCm'C

Cuts at:      0      49    1176 
   Size:         49    1127
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     890    1176 
   Size:        890     286
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     903    1176 
   Size:        903     273
 
Bpu10I CC'TnA_GC

Cuts at:      0     772    1176 
   Size:        772     404
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317    1176 
   Size:        317     859
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1016    1176 
   Size:       1016     160
 
BsaAI yAC'GTr

Cuts at:      0     856    1176 
   Size:        856     320
 
BsaBI GATnn'nnATC

Cuts at:      0     290     882    1176 
   Size:        290     592     294

  Fragments arranged by size: 

                 592     294     290
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     789     882    1011    1158    1176 
   Size:        789      93     129     147      18

  Fragments arranged by size: 

                 789     147     129      93      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625    1176 
   Size:        625     551
 
BsiHKAI G_wGCw'C

Cuts at:      0    1150    1176 
   Size:       1150      26
 
Bsp1286I G_dGCh'C

Cuts at:      0      49    1150    1176 
   Size:         49    1101      26

  Fragments arranged by size: 

                1101      49      26
 
BspHI T'CATG_A

Cuts at:      0     135     378     597    1176 
   Size:        135     243     219     579

  Fragments arranged by size: 

                 579     243     219     135
 
BsrBI CCG'CTC

Cuts at:      0     205     917    1176 
   Size:        205     712     259

  Fragments arranged by size: 

                 712     259     205
 
BsrDI GCAATG_nn'

Cuts at:      0     278    1176 
   Size:        278     898
 
BssHII G'CGCG_C

Cuts at:      0      21    1176 
   Size:         21    1155
 
BssSI C'ACGA_G

Cuts at:      0    1076    1176 
   Size:       1076     100
 
BstYI r'GATC_y

Cuts at:      0     162     808    1000    1176 
   Size:        162     646     192     176

  Fragments arranged by size: 

                 646     192     176     162
 
BtgI C'CryG_G

Cuts at:      0     257    1176 
   Size:        257     919
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      40     811     886    1176 
   Size:         40     771      75     290

  Fragments arranged by size: 

                 771     290      75      40
 
ClaI AT'CG_AT

Cuts at:      0     837    1176 
   Size:        837     339
 
DraIII CAC_nnn'GTG

Cuts at:      0    1078    1176 
   Size:       1078      98
 
EaeI y'GGCC_r

Cuts at:      0     308    1176 
   Size:        308     868
 
EarI CTCTTCn'nnn_

Cuts at:      0     332     732     737    1148    1176 
   Size:        332     400       5     411      28

  Fragments arranged by size: 

                 411     400     332      28       5
 
EcoICRI GAG'CTC

Cuts at:      0    1148    1176 
   Size:       1148      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     371     455     903    1172    1176 
   Size:        371      84     448     269       4

  Fragments arranged by size: 

                 448     371     269      84       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0     779     911    1176 
   Size:        779     132     265

  Fragments arranged by size: 

                 779     265     132
 
EcoRI G'AATT_C

Cuts at:      0     522    1176 
   Size:        522     654
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1021    1053    1176 
   Size:       1021      32     123

  Fragments arranged by size: 

                1021     123      32
 
HincII GTy'rAC

Cuts at:      0      76    1176 
   Size:         76    1100
 
HindIII A'AGCT_T

Cuts at:      0     670    1176 
   Size:        670     506
 
MscI TGG'CCA

Cuts at:      0     310    1176 
   Size:        310     866
 
MslI CAynn'nnrTG

Cuts at:      0     602    1176 
   Size:        602     574
 
NcoI C'CATG_G

Cuts at:      0     257    1176 
   Size:        257     919
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      42     192    1176 
   Size:         42     150     984

  Fragments arranged by size: 

                 984     150      42
 
PmlI CAC'GTG

Cuts at:      0     856    1176 
   Size:        856     320
 
PpuMI rG'GwC_Cy

Cuts at:      0     779     911    1176 
   Size:        779     132     265

  Fragments arranged by size: 

                 779     265     132
 
PstI C_TGCA'G

Cuts at:      0     272    1176 
   Size:        272     904
 
SacI G_AGCT'C

Cuts at:      0    1150    1176 
   Size:       1150      26
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1148    1176 
   Size:       1148      28
 
SfcI C'TryA_G

Cuts at:      0     268    1176 
   Size:        268     908
 
SmaI CCC'GGG

Cuts at:      0      55    1176 
   Size:         55    1121
 
SmlI C'TyrA_G

Cuts at:      0     332    1176 
   Size:        332     844
 
StyI C'CwwG_G

Cuts at:      0     257     413    1176 
   Size:        257     156     763

  Fragments arranged by size: 

                 763     257     156
 
XbaI T'CTAG_A

Cuts at:      0     967    1176 
   Size:        967     209
 
XmaI C'CCGG_G

Cuts at:      0      53    1176 
   Size:         53    1123
 
XmnI GAAnn'nnTTC

Cuts at:      0     374    1176 
   Size:        374     802
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AvaI     BanI    BanII     BbsI    BbvCI
     BcgI     BclI Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrBI
    BsrDI   BssHII    BssSI    BstYI     BtgI    BtgZI     ClaI   DraIII
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    Hin4I   HincII
  HindIII     MscI     MslI     NcoI    PflMI     PmlI    PpuMI     PstI
     SacI     SapI     SfcI     SmaI     SmlI     StyI     XbaI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrFI    BsrGI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtsI    CspCI     DraI     DrdI     EagI     EciI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     ZraI
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