Restriction map for pF1KB6476
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BcgI        BclI        BcnI        
BfrI        BglI        BglII       BlnI        BpmI        
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AlwNI         724  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       190  GAATATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          334  CTCGGG               C^[CT]CG[GA]G
BanI           44  GGTGCC               G^G[CT][GA]CC
BbvCI         397  GCTGAGG              CCTCAGC(-5/-2)
BciVI         277  GGATAC               GTATCC(6/5)
BlpI          220  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      220  GCTAAGC              GC^T[ACGT]AGC
BsaAI        1036  TACGTG               [CT]AC^GT[GA]
BsaI          898  GGTCTC               GGTCTC(1/5)
BsmAI         899  GTCTC                GTCTC(1/5)
BsmI          230  GAATGC               GAATGC(1/-1)
BsoBI         334  CTCGGG               C^[CT]CG[GA]G
BspMI         261  GCAGGT               ACCTGC(4/8)
BsrDI         356  GCAATG               GCAATG(2/0)
BstAPI        723  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         251  AGATCC               [GA]^GATC[CT]
CelII         220  GCTAAGC              GC^T[ACGT]AGC
DrdI          562  GACGAAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          875  CCATGG               C^C[GA][CT]GG
EarI          595  GAAGAG               CTCTTC(1/4)
EcoT22I       359  ATGCAT               ATGCA^T
FauI          282  CCCGC                CCCGC(4/6)
Ksp632I       595  GAAGAG               CTCTTC(1/4)
MaeII        1037  ACGT                 A^CGT
MflI          251  AGATCC               [GA]^GATC[CT]
MslI          870  CATTCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        758  CAGCTG               C[AC]G^C[GT]G
NcoI          875  CCATGG               C^CATGG
NlaIV          44  GGTGCC               GG[ACGT]^[ACGT]CC
NsiI          359  ATGCAT               ATGCA^T
NspI          623  GCATGT               [GA]CATG^[CT]
PvuII         758  CAGCTG               CAG^CTG
StuI          796  AGGCCT               AGG^CCT
TaqI          247  TCGA                 T^CGA
TthHB8I       247  TCGA                 T^CGA
XhoII         251  AGATCC               [GA]^GATC[CT]
XmnI          190  GAATATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwI          252  GATCC                GGATC(4/5)
              445  GATCC               
AseI          421  ATTAAT               AT^TAAT
             1030  ATTAAT              
AsnI          421  ATTAAT               AT^TAAT
             1030  ATTAAT              
AspHI         331  GAGCTC               G[AT]GC[AT]^C
              820  GAGCTC              
AvaII          56  GGACC                G^G[AT]CC
              879  GGACC               
BanII         331  GAGCTC               G[GA]GC[CT]^C
              820  GAGCTC              
BmyI          331  GAGCTC               G[AGT]GC[ACT]^C
              820  GAGCTC              
BsiHKAI       331  GAGCTC               G[AT]GC[AT]^C
              820  GAGCTC              
Bsp1286I      331  GAGCTC               G[AGT]GC[ACT]^C
              820  GAGCTC              
BssKI          53  CCTGG                ^CC[ACGT]GG
              519  CCTGG               
BstF5I        764  CATCC                GGATG(2/0)
                3  GGATG               
BstNI          53  CCTGG                CC^[AT]GG
              519  CCTGG               
Cac8I         296  GCTTGC               GC[ACGT]^[ACGT]GC
              737  GCAAGC              
Cfr13I         56  GGACC                G^G[ACGT]CC
              879  GGACC               
DraI          474  TTTAAA               TTT^AAA
              940  TTTAAA              
EcoRII         53  CCTGG                ^CC[AT]GG
              519  CCTGG               
FokI          764  CATCC                GGATG(9/13)
                3  GGATG               
HaeIII        511  GGCC                 GG^CC
              797  GGCC                
MaeIII        148  GTTAC                ^GT[ACGT]AC
              456  GTTAC               
MvaI           53  CCTGG                CC^[AT]GG
              519  CCTGG               
PshBI         421  ATTAAT               AT^TAAT
             1030  ATTAAT              
PstI          721  CTGCAG               CTGCA^G
              730  CTGCAG              
SacI          331  GAGCTC               GAGCT^C
              820  GAGCTC              
Sau96I         56  GGACC                G^G[ACGT]CC
              879  GGACC               
ScrFI          53  CCTGG                CC^[ACGT]GG
              519  CCTGG               
XcmI          383  CCATGAAGCTAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1028  CCATTAATTACGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BbsI        
BbvI        BfaI        BpuAI       BsaJI       BsiYI       
BslI        BsmFI       BsrI        DdeI        DpnII       
EcoT14I     Fnu4HI      HinfI       HphI        ItaI        
MaeI        MboI        MboII       MnlI        MseI        
MwoI        NdeII       NlaIII      PleI        Sau3AI      
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com