Restriction map for pF1KB6469
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AccI        AccII       AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspHI       AspI        AvaI        AviII       BalI        
BamHI       BanII       BbeI        BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlpI        
BpmI        Bpu1102I    BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        HinP1I      HincII      HindII      HpaI        
HphI        KasI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         694  GACGTC               GACGT^C
Acc65I        281  GGTACC               G^GTACC
AciI          567  GCGG                 CCGC(-3/-1)
AcyI          694  GACGTC               G[GA]^CG[CT]C
Asp718I       281  GGTACC               G^GTACC
AvrII         390  CCTAGG               C^CTAGG
BanI          281  GGTACC               G^G[CT][GA]CC
BbrPI         469  CACGTG               CAC^GTG
BbvCI         499  GCTGAGG              CCTCAGC(-5/-2)
BcnI          846  CCGGG                CC^[GC]GG
BfaI          391  CTAG                 C^TAG
BlnI          390  CCTAGG               C^CTAGG
BmyI          552  GGGCAC               G[AGT]GC[ACT]^C
BsaAI         469  CACGTG               [CT]AC^GT[GA]
BsaHI         694  GACGTC               G[GA]^CG[CT]C
BseRI         952  GAGGAG               GAGGAG(10/8)
Bsp1286I      552  GGGCAC               G[AGT]GC[ACT]^C
BspHI         332  TCATGA               T^CATGA
BspLU11I      230  ACATGT               A^CATGT
BspMI         704  ACCTGC               ACCTGC(4/8)
BsrDI         315  CATTGC               GCAATG(2/0)
BsrI          476  CCAGT                ACTGG(1/-1)
BstF5I        849  GGATG                GGATG(2/0)
EarI          880  GAAGAG               CTCTTC(1/4)
FokI          849  GGATG                GGATG(9/13)
Hin1I         694  GACGTC               G[GA]^CG[CT]C
HindIII       610  AAGCTT               A^AGCTT
KpnI          281  GGTACC               GGTAC^C
Ksp632I       880  GAAGAG               CTCTTC(1/4)
MaeI          391  CTAG                 C^TAG
MseI          245  TTAA                 T^TAA
NciI          846  CCGGG                CC^[GC]GG
NcoI          585  CCATGG               C^CATGG
NlaIV         281  GGTACC               GG[ACGT]^[ACGT]CC
NspI          230  ACATGT               [GA]CATG^[CT]
PmaCI         469  CACGTG               CAC^GTG
PmlI          469  CACGTG               CAC^GTG
PpuMI         386  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         685  CAGCTG               CAG^CTG
RcaI          332  TCATGA               T^CATGA
SbfI          723  CCTGCAGG             CCTGCA^GG
ScaI          138  AGTACT               AGT^ACT
SexAI         578  ACCAGGT              A^CC[AT]GGT
SfaNI         850  GATGC                GCATC(5/9)
Sse8387I      723  CCTGCAGG             CCTGCA^GG
StuI          740  AGGCCT               AGG^CCT
Tru9I         245  TTAA                 T^TAA
Tsp45I        472  GTGAC                ^GT[GC]AC
XcmI          758  CCAATGACCAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII        230  ACATGT               A^C[GA][CT]GT
              468  ACACGT              
BbsI          753  GAAGAC               GAAGAC(2/6)
              697  GTCTTC              
BpuAI         753  GAAGAC               GAAGAC(2/6)
              697  GTCTTC              
BsgI          814  CTGCAC               GTGCAG(16/14)
               16  GTGCAG              
BsmFI         597  GGGAC                GGGAC(10/14)
              561  GTCCC               
DdeI           49  CTCAG                C^T[ACGT]AG
              500  CTGAG               
DraII         291  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              386  AGGACCT             
DsaI          585  CCATGG               C^C[GA][CT]GG
              803  CCGTGG              
EcoO109I      291  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              386  AGGACCT             
EcoRV         532  GATATC               GAT^ATC
              619  GATATC              
EcoT14I       390  CCTAGG               C^C[AT][AT]GG
              585  CCATGG              
HapII           4  CCGG                 C^CGG
              846  CCGG                
HinfI          74  GAATC                G^A[ACGT]TC
              193  GATTC               
HpaII           4  CCGG                 C^CGG
              846  CCGG                
MaeII         470  ACGT                 A^CGT
              695  ACGT                
MaeIII        472  GTGAC                ^GT[ACGT]AC
              525  GTTAC               
MspA1I        565  CAGCGG               C[AC]G^C[GT]G
              685  CAGCTG              
MspI            4  CCGG                 C^CGG
              846  CCGG                
MwoI            9  GCTGGCAGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              490  GCAGAGGAAGC         
PflMI          33  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              765  CCAGATGGTGG         
PstI          688  CTGCAG               CTGCA^G
              724  CTGCAG              
StyI          390  CCTAGG               C^C[AT][AT]GG
              585  CCATGG              
TaqI          151  TCGA                 T^CGA
              454  TCGA                
TfiI           74  GAATC                G^A[AT]TC
              193  GATTC               
Tsp509I       352  AATT                 ^AATT
              483  AATT                
TthHB8I       151  TCGA                 T^CGA
              454  TCGA                
Van91I         33  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              765  CCAGATGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AfaI        AluI        AlwI        AvaII       BbvI        
BsaJI       BsiYI       BslI        BssKI       BstNI       
BstXI       Cac8I       Cfr13I      DpnII       EcoRII      
Fnu4HI      HaeIII      ItaI        MboI        MboII       
MnlI        MslI        MvaI        NdeII       NlaIII      
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
TseI        TspRI       
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