Restriction map for pF1KB6469
 (Linear) (Six-base) MAPSORT of: KIBB6469.seq  Check: 8106  from: 1  to: 972



KIBB6469.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AatII G_ACGT'C

Cuts at:      0     698     972 
   Size:        698     274
 
Acc65I G'GTAC_C

Cuts at:      0     281     972 
   Size:        281     691
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772     972 
   Size:        772     200
 
AflIII A'CryG_T

Cuts at:      0     230     468     972 
   Size:        230     238     504

  Fragments arranged by size: 

                 504     238     230
 
AleI CACnn'nnGTG

Cuts at:      0     547     972 
   Size:        547     425
 
AvrII C'CTAG_G

Cuts at:      0     390     972 
   Size:        390     582
 
BanI G'GyrC_C

Cuts at:      0     281     972 
   Size:        281     691
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     690     760     972 
   Size:        690      70     212

  Fragments arranged by size: 

                 690     212      70
 
BbvCI CC'TCA_GC

Cuts at:      0     500     972 
   Size:        500     472
 
Bme1580I G_kGCm'C

Cuts at:      0     556     972 
   Size:        556     416
 
Bpu10I CC'TnA_GC

Cuts at:      0     500     972 
   Size:        500     472
 
BsaAI yAC'GTr

Cuts at:      0     471     972 
   Size:        471     501
 
BsaHI Gr'CG_yC

Cuts at:      0     695     972 
   Size:        695     277
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     967     972 
   Size:        967       5
 
BseYI C'CCAG_C

Cuts at:      0     563     972 
   Size:        563     409
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     799     972 
   Size:         37     762     173

  Fragments arranged by size: 

                 762     173      37
 
Bsp1286I G_dGCh'C

Cuts at:      0     556     972 
   Size:        556     416
 
BspHI T'CATG_A

Cuts at:      0     332     972 
   Size:        332     640
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     713     972 
   Size:        713     259
 
BsrDI GCAATG_nn'

Cuts at:      0     314     972 
   Size:        314     658
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     439     586     615     972 
   Size:        439     147      29     357

  Fragments arranged by size: 

                 439     357     147      29
 
BtgI C'CryG_G

Cuts at:      0     585     803     972 
   Size:        585     218     169

  Fragments arranged by size: 

                 585     218     169
 
BtsI GCAGTG_nn'

Cuts at:      0      20     972 
   Size:         20     952
 
EarI CTCTTCn'nnn_

Cuts at:      0     875     972 
   Size:        875      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772     972 
   Size:        772     200
 
EcoO109I rG'GnC_Cy

Cuts at:      0     292     387     972 
   Size:        292      95     585

  Fragments arranged by size: 

                 585     292      95
 
EcoRV GAT'ATC

Cuts at:      0     534     621     972 
   Size:        534      87     351

  Fragments arranged by size: 

                 534     351      87
 
HindIII A'AGCT_T

Cuts at:      0     610     972 
   Size:        610     362
 
KpnI G_GTAC'C

Cuts at:      0     285     972 
   Size:        285     687
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     472     489     972 
   Size:         66     406      17     483

  Fragments arranged by size: 

                 483     406      66      17
 
MslI CAynn'nnrTG

Cuts at:      0     308     373     418     437     547     972 
   Size:        308      65      45      19     110     425

  Fragments arranged by size: 

                 425     308     110      65      45      19
 
MspA1I CmG'CkG

Cuts at:      0     567     687     972 
   Size:        567     120     285

  Fragments arranged by size: 

                 567     285     120
 
NcoI C'CATG_G

Cuts at:      0     585     972 
   Size:        585     387
 
NspI r_CATG'y

Cuts at:      0     234     972 
   Size:        234     738
 
PciI A'CATG_T

Cuts at:      0     230     972 
   Size:        230     742
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      39     771     972 
   Size:         39     732     201

  Fragments arranged by size: 

                 732     201      39
 
PmlI CAC'GTG

Cuts at:      0     471     972 
   Size:        471     501
 
PpuMI rG'GwC_Cy

Cuts at:      0     387     972 
   Size:        387     585
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     509     541     972 
   Size:        509      32     431

  Fragments arranged by size: 

                 509     431      32
 
PstI C_TGCA'G

Cuts at:      0     692     728     972 
   Size:        692      36     244

  Fragments arranged by size: 

                 692     244      36
 
PvuII CAG'CTG

Cuts at:      0     687     972 
   Size:        687     285
 
SbfI CC_TGCA'GG

Cuts at:      0     728     972 
   Size:        728     244
 
ScaI AGT'ACT

Cuts at:      0     140     972 
   Size:        140     832
 
SexAI A'CCwGG_T

Cuts at:      0     578     972 
   Size:        578     394
 
SfcI C'TryA_G

Cuts at:      0     401     688     724     972 
   Size:        401     287      36     248

  Fragments arranged by size: 

                 401     287     248      36
 
StuI AGG'CCT

Cuts at:      0     742     972 
   Size:        742     230
 
StyI C'CwwG_G

Cuts at:      0     390     585     972 
   Size:        390     195     387

  Fragments arranged by size: 

                 390     387     195
 
TatI w'GTAC_w

Cuts at:      0     138     779     972 
   Size:        138     641     193

  Fragments arranged by size: 

                 641     193     138
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     765     972 
   Size:        765     207
 
ZraI GAC'GTC

Cuts at:      0     696     972 
   Size:        696     276
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI   AflIII     AleI    AvrII     BanI     BbsI
    BbvCI Bme1580I   Bpu10I    BsaAI    BsaHI    BseRI    BseYI     BsgI
 Bsp1286I    BspHI    BspMI    BsrDI    BstXI     BtgI     BtsI     EarI
  Eco57MI EcoO109I    EcoRV  HindIII     KpnI     MmeI     MslI   MspA1I
     NcoI     NspI     PciI    PflMI     PmlI    PpuMI     PsrI     PstI
    PvuII     SbfI     ScaI    SexAI     SfcI     StuI     StyI     TatI
     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI    BanII     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI
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