Restriction map for pF1KB6438
 (Linear) (Six-base) MAPSORT of: KIBB6438.seq  Check: 1083  from: 1  to: 1020



KIBB6438.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     271     647    1020 
   Size:        271     376     373

  Fragments arranged by size: 

                 376     373     271
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     868     870    1020 
   Size:        523     345       2     150

  Fragments arranged by size: 

                 523     345     150       2
 
AflIII A'CryG_T

Cuts at:      0     185    1020 
   Size:        185     835
 
AleI CACnn'nnGTG

Cuts at:      0     352    1020 
   Size:        352     668
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     253    1020 
   Size:        253     767
 
AlwNI CAG_nnn'CTG

Cuts at:      0     795     957    1020 
   Size:        795     162      63

  Fragments arranged by size: 

                 795     162      63
 
ApaI G_GGCC'C

Cuts at:      0     176     311     574     821    1020 
   Size:        176     135     263     247     199

  Fragments arranged by size: 

                 263     247     199     176     135
 
AvaI C'yCGr_G

Cuts at:      0     311    1020 
   Size:        311     709
 
BanI G'GyrC_C

Cuts at:      0      39     109     596    1020 
   Size:         39      70     487     424

  Fragments arranged by size: 

                 487     424      70      39
 
BanII G_rGCy'C

Cuts at:      0      20     176     311     574     821    1020 
   Size:         20     156     135     263     247     199

  Fragments arranged by size: 

                 263     247     199     156     135      20
 
BbeI G_GCGC'C

Cuts at:      0     600    1020 
   Size:        600     420
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     928    1020 
   Size:        928      92
 
BglI GCCn_nnn'nGGC

Cuts at:      0     171     798    1020 
   Size:        171     627     222

  Fragments arranged by size: 

                 627     222     171
 
BlpI GC'TnA_GC

Cuts at:      0     743    1020 
   Size:        743     277
 
Bme1580I G_kGCm'C

Cuts at:      0     112     176     311     530     574     586     666     821 
   Size:        112      64     135     219      44      12      80     155

Cuts at:    821    1020 
   Size:        199

  Fragments arranged by size: 

                 219     199     155     135     112      80      64      44
                  12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     815     885    1020 
   Size:        815      70     135

  Fragments arranged by size: 

                 815     135      70
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     516    1020 
   Size:         97     419     504

  Fragments arranged by size: 

                 504     419      97
 
Bpu10I CC'TnA_GC

Cuts at:      0      14     835    1020 
   Size:         14     821     185

  Fragments arranged by size: 

                 821     185      14
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377    1020 
   Size:        377     643
 
BsaAI yAC'GTr

Cuts at:      0     188     667     916    1020 
   Size:        188     479     249     104

  Fragments arranged by size: 

                 479     249     188     104
 
BsaHI Gr'CG_yC

Cuts at:      0     221     597     881    1020 
   Size:        221     376     284     139

  Fragments arranged by size: 

                 376     284     221     139
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     946     949     952    1020 
   Size:        946       3       3      68

  Fragments arranged by size: 

                 946      68       3       3
 
BseYI C'CCAG_C

Cuts at:      0      46      55     323    1020 
   Size:         46       9     268     697

  Fragments arranged by size: 

                 697     268      46       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     985    1020 
   Size:        985      35
 
BsiHKAI G_wGCw'C

Cuts at:      0      20     944    1020 
   Size:         20     924      76

  Fragments arranged by size: 

                 924      76      20
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     112     176     311     530     574     586     666 
   Size:         20      92      64     135     219      44      12      80

Cuts at:    666     821     944    1020 
   Size:        155     123      76

  Fragments arranged by size: 

                 219     155     135     123      92      80      76      64
                  44      20      12
 
BspHI T'CATG_A

Cuts at:      0     452    1020 
   Size:        452     568
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     271     623     647    1020 
   Size:        271     352      24     373

  Fragments arranged by size: 

                 373     352     271      24
 
BsrBI CCG'CTC

Cuts at:      0     342    1020 
   Size:        342     678
 
BsrDI GCAATG_nn'

Cuts at:      0     249    1020 
   Size:        249     771
 
BssHII G'CGCG_C

Cuts at:      0      51     245     281     738    1020 
   Size:         51     194      36     457     282

  Fragments arranged by size: 

                 457     282     194      51      36
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     116     795    1020 
   Size:        116     679     225

  Fragments arranged by size: 

                 679     225     116
 
BstYI r'GATC_y

Cuts at:      0     953     986    1020 
   Size:        953      33      34

  Fragments arranged by size: 

                 953      34      33
 
BtgI C'CryG_G

Cuts at:      0     147     167     332    1020 
   Size:        147      20     165     688

  Fragments arranged by size: 

                 688     165     147      20
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      84    1020 
   Size:         84     936
 
BtsI GCAGTG_nn'

Cuts at:      0     247    1020 
   Size:        247     773
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     533     568    1020 
   Size:        533      35     452

  Fragments arranged by size: 

                 533     452      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     116     191    1020 
   Size:        116      75     829

  Fragments arranged by size: 

                 829     116      75
 
EaeI y'GGCC_r

Cuts at:      0     144     150     753    1020 
   Size:        144       6     603     267

  Fragments arranged by size: 

                 603     267     144       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     454    1020 
   Size:        454     566
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     937    1020 
   Size:        937      83
 
EcoICRI GAG'CTC

Cuts at:      0      18    1020 
   Size:         18    1002
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     516     523     868     870    1020 
   Size:         97     419       7     345       2     150

  Fragments arranged by size: 

                 419     345     150      97       7       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     619    1020 
   Size:        619     401
 
EcoO109I rG'GnC_Cy

Cuts at:      0     172     308     315     570     681     817     831     996 
   Size:        172     136       7     255     111     136      14     165

Cuts at:    996    1020 
   Size:         24

  Fragments arranged by size: 

                 255     172     165     136     136     111      24      14
                   7
 
FspI TGC'GCA

Cuts at:      0      97     229    1020 
   Size:         97     132     791

  Fragments arranged by size: 

                 791     132      97
 
HaeII r_GCGC'y

Cuts at:      0     600    1020 
   Size:        600     420
 
KasI G'GCGC_C

Cuts at:      0     596    1020 
   Size:        596     424
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     758    1020 
   Size:        758     262
 
MscI TGG'CCA

Cuts at:      0     152    1020 
   Size:        152     868
 
MslI CAynn'nnrTG

Cuts at:      0     352     641    1020 
   Size:        352     289     379

  Fragments arranged by size: 

                 379     352     289
 
MspA1I CmG'CkG

Cuts at:      0      46     135     169     275     334     501     927    1020 
   Size:         46      89      34     106      59     167     426      93

  Fragments arranged by size: 

                 426     167     106      93      89      59      46      34
            
 
NarI GG'CG_CC

Cuts at:      0     597    1020 
   Size:        597     423
 
NspI r_CATG'y

Cuts at:      0     646     894    1020 
   Size:        646     248     126

  Fragments arranged by size: 

                 646     248     126
 
PmlI CAC'GTG

Cuts at:      0     188     667    1020 
   Size:        188     479     353

  Fragments arranged by size: 

                 479     353     188
 
PpuMI rG'GwC_Cy

Cuts at:      0     315    1020 
   Size:        315     705
 
PspOMI G'GGCC_C

Cuts at:      0     172     307     570     817    1020 
   Size:        172     135     263     247     203

  Fragments arranged by size: 

                 263     247     203     172     135
 
PstI C_TGCA'G

Cuts at:      0     242     926     962    1020 
   Size:        242     684      36      58

  Fragments arranged by size: 

                 684     242      58      36
 
PvuII CAG'CTG

Cuts at:      0     135     501    1020 
   Size:        135     366     519

  Fragments arranged by size: 

                 519     366     135
 
SacI G_AGCT'C

Cuts at:      0      20    1020 
   Size:         20    1000
 
SacII CC_GC'GG

Cuts at:      0     170     335    1020 
   Size:        170     165     685

  Fragments arranged by size: 

                 685     170     165
 
ScaI AGT'ACT

Cuts at:      0     967    1020 
   Size:        967      53
 
SfcI C'TryA_G

Cuts at:      0     238     922     958    1020 
   Size:        238     684      36      62

  Fragments arranged by size: 

                 684     238      62      36
 
SfoI GGC'GCC

Cuts at:      0     598    1020 
   Size:        598     422
 
SmaI CCC'GGG

Cuts at:      0     313    1020 
   Size:        313     707
 
SmlI C'TyrA_G

Cuts at:      0     392    1020 
   Size:        392     628
 
SnaBI TAC'GTA

Cuts at:      0     916    1020 
   Size:        916     104
 
SphI G_CATG'C

Cuts at:      0     894    1020 
   Size:        894     126
 
StuI AGG'CCT

Cuts at:      0     236    1020 
   Size:        236     784
 
StyI C'CwwG_G

Cuts at:      0     620    1014    1020 
   Size:        620     394       6

  Fragments arranged by size: 

                 620     394       6
 
TatI w'GTAC_w

Cuts at:      0     422     761     965    1020 
   Size:        422     339     204      55

  Fragments arranged by size: 

                 422     339     204      55
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     132    1020 
   Size:        132     888
 
XmaI C'CCGG_G

Cuts at:      0     311    1020 
   Size:        311     709
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI     AvaI
     BanI    BanII     BbeI    BciVI     BglI     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI   BssHII   BstAPI
    BstYI     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII     KasI
     MmeI     MscI     MslI   MspA1I     NarI     NspI     PmlI    PpuMI
   PspOMI     PstI    PvuII     SacI    SacII     ScaI     SfcI     SfoI
     SmaI     SmlI    SnaBI     SphI     StuI     StyI     TatI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII    BmgBI
     BmtI     BplI     BsaI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrFI    BsrGI    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI     DraI     DrdI     EagI    EcoRI
    EcoRV     FalI     FseI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI
     SpeI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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