Restriction map for pF1KB6405
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

Acc65I      AclI        AcsI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbsI        BciVI       BclI        
BcnI        BfrI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaI        BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspLU11I    BspMI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       BstBI       
BstEII      BstPI       Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MspA1I      MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsaI        RsrII       SacII       SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         733  GACGTC               GACGT^C
AccII         777  CGCG                 CG^CG
AccIII         80  TCCGGA               T^CCGGA
AcyI          733  GACGTC               G[GA]^CG[CT]C
AlwI           49  GATCC                GGATC(4/5)
Asp700I       292  GAACTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         399  GGTCC                G^G[AT]CC
BalI          491  TGGCCA               TGG^CCA
BcgI          819  CGACTACACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          535  CTAG                 C^TAG
BglI          421  GCCGTGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         733  GACGTC               G[GA]^CG[CT]C
BsaWI          80  TCCGGA               [AT]^CCGG[AT]
BseAI          80  TCCGGA               T^CCGGA
BspEI          80  TCCGGA               T^CCGGA
BspHI         275  TCATGA               T^CATGA
BsrBI         283  GAGCGG               CCGCTC(-3/-3)
Bst1107I      448  GTATAC               GTA^TAC
BstAPI         67  GCAGTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         777  CGCG                 CG^CG
BstXI         635  CCAACCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       448  GTATAC               GTA^TAC
Cac8I         866  GCGTGC               GC[ACGT]^[ACGT]GC
CfoI          778  GCGC                 GCG^C
Cfr13I        399  GGTCC                G^G[ACGT]CC
EcoNI         795  CCTCAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI          778  GCGC                 GCG^C
Hin1I         733  GACGTC               G[GA]^CG[CT]C
HinP1I        778  GCGC                 G^CGC
HincII        817  GTCGAC               GT[CT]^[GA]AC
HindII        817  GTCGAC               GT[CT]^[GA]AC
HphI          146  TCACC                GGTGA(8/7)
MaeI          535  CTAG                 C^TAG
MluNI         491  TGGCCA               TGG^CCA
MroI           80  TCCGGA               T^CCGGA
MscI          491  TGGCCA               TGG^CCA
MslI          525  CACTGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          777  CGCG                 CG^CG
NlaIV         631  GGAACC               GG[ACGT]^[ACGT]CC
RcaI          275  TCATGA               T^CATGA
SalI          817  GTCGAC               G^TCGAC
SapI          210  GCTCTTC              GCTCTTC(1/4)
Sau96I        399  GGTCC                G^G[ACGT]CC
SmlI          769  CTCAAG               C^T[CT][GA]AG
SspI          372  AATATT               AAT^ATT
TaqI          818  TCGA                 T^CGA
TfiI          141  GAATC                G^A[AT]TC
TthHB8I       818  TCGA                 T^CGA
XmnI          292  GAACTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          448  GTATAC               GT^[AC][GT]AC
              817  GTCGAC              
AlwNI          68  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              127  CAGGGTCTG           
AspHI         208  GAGCTC               G[AT]GC[AT]^C
              895  GAGCTC              
BanII         208  GAGCTC               G[GA]GC[CT]^C
              895  GAGCTC              
BbvCI          62  CCTCAGC              CCTCAGC(-5/-2)
              878  CCTCAGC             
BglII         336  AGATCT               A^GATCT
              570  AGATCT              
BmyI          208  GAGCTC               G[AGT]GC[ACT]^C
              895  GAGCTC              
BpmI          390  CTCCAG               CTGGAG(16/14)
               91  CTGGAG              
BseRI         180  CTCCTC               GAGGAG(10/8)
              802  GAGGAG              
BsiHKAI       208  GAGCTC               G[AT]GC[AT]^C
              895  GAGCTC              
BsiYI         197  CCTCTGGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              795  CCTCAAAGAGG         
BslI          197  CCTCTGGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              795  CCTCAAAGAGG         
BsmAI         118  GAGAC                GTCTC(1/5)
               33  GTCTC               
BsmFI         517  GTCCC                GGGAC(10/14)
              736  GTCCC               
Bsp1286I      208  GAGCTC               G[AGT]GC[ACT]^C
              895  GAGCTC              
BsrI          784  ACTGG                ACTGG(1/-1)
              149  CCAGT               
BstF5I         78  CATCC                GGATG(2/0)
              808  CATCC               
DsaI          422  CCGTGG               C^C[GA][CT]GG
              811  CCGTGG              
EaeI          428  CGGCCA               [CT]^GGCC[GA]
              491  TGGCCA              
EarI          211  CTCTTC               CTCTTC(1/4)
              102  GAAGAG              
FauI          203  GCGGG                CCCGC(4/6)
              285  GCGGG               
FokI           78  CATCC                GGATG(9/13)
              808  CATCC               
HapII          81  CCGG                 C^CGG
              708  CCGG                
HpaII          81  CCGG                 C^CGG
              708  CCGG                
Ksp632I       211  CTCTTC               CTCTTC(1/4)
              102  GAAGAG              
MspI           81  CCGG                 C^CGG
              708  CCGG                
SacI          208  GAGCTC               GAGCT^C
              895  GAGCTC              
SfaNI         538  GATGC                GCATC(5/9)
              807  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BssKI       
BstNI       BstYI       DdeI        DpnII       EcoRII      
Fnu4HI      HaeIII      HinfI       ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
PleI        Sau3AI      ScrFI       Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       
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