Restriction map for pF1KB6405
 (Circular) (Six-base) MAPSORT of: pF1KB6405.seq  Check: 2500  from: 1  to: 4028



pF1KB6405  4028 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AatII G_ACGT'C

Cuts at:    845     845 
   Size:       4028
 
AccI GT'mk_AC

Cuts at:    557     926    1066     557 
   Size:        369     140    3519

  Fragments arranged by size: 

                3519     369     140
 
Acc65I G'GTAC_C

Cuts at:   1044    1044 
   Size:       4028
 
AclI AA'CG_TT

Cuts at:   3868    3868 
   Size:       4028
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    719    1853    2285    3032    3300     719 
   Size:       1134     432     747     268    1447

  Fragments arranged by size: 

                1447    1134     747     432     268
 
AflIII A'CryG_T

Cuts at:   1380    2484    3461    1380 
   Size:       1104     977    1947

  Fragments arranged by size: 

                1947    1104     977
 
AgeI A'CCGG_T

Cuts at:   2416    2416 
   Size:       4028
 
AleI CACnn'nnGTG

Cuts at:    637     637 
   Size:       4028
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    465     465 
   Size:       4028
 
AlwNI CAG_nnn'CTG

Cuts at:    181     240    1333    2900     181 
   Size:         59    1093    1567    1309

  Fragments arranged by size: 

                1567    1309    1093      59
 
ApaLI G'TGCA_C

Cuts at:   2798    2798 
   Size:       4028
 
ApoI r'AATT_y

Cuts at:   1032    3381    3637    1032 
   Size:       2349     256    1423

  Fragments arranged by size: 

                2349    1423     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4028
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4028
 
AvaI C'yCGr_G

Cuts at:   1048    1048 
   Size:       4028
 
BamHI G'GATC_C

Cuts at:   1053    1053 
   Size:       4028
 
BanI G'GyrC_C

Cuts at:   1044    1458    1706    1741    1044 
   Size:        414     248      35    3331

  Fragments arranged by size: 

                3331     414     248      35
 
BanII G_rGCy'C

Cuts at:    320    1007    1042    2072     320 
   Size:        687      35    1030    2276

  Fragments arranged by size: 

                2276    1030     687      35
 
BbeI G_GCGC'C

Cuts at:   1462    1710    1462 
   Size:        248    3780
 
BbvCI CC'TCA_GC

Cuts at:    171     987     171 
   Size:        816    3212
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    916     950     916 
   Size:         34    3994
 
BciVI GTATCCnnnnn_n'

Cuts at:   1926    2687    4028    1926 
   Size:        761    1341    1926

  Fragments arranged by size: 

                1926    1341     761
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       4028
 
BglI GCCn_nnn'nGGC

Cuts at:    535     535 
   Size:       4028
 
BglII A'GATC_T

Cuts at:    444     678    1543    3580     444 
   Size:        234     865    2037     892

  Fragments arranged by size: 

                2037     892     865     234
 
BlpI GC'TnA_GC

Cuts at:   1140    1140 
   Size:       4028
 
Bme1580I G_kGCm'C

Cuts at:   1653    1746    2802    1653 
   Size:         93    1056    2879

  Fragments arranged by size: 

                2879    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1240    1321    1656    3800    1240 
   Size:         81     335    2144    1468

  Fragments arranged by size: 

                2144    1468     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2395    2395 
   Size:       4028
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    220     483     220 
   Size:        263    3765
 
Bpu10I CC'TnA_GC

Cuts at:    171     987     171 
   Size:        816    3212
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    862    1204    2083    2575    2873     862 
   Size:        342     879     492     298    2017

  Fragments arranged by size: 

                2017     879     492     342     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3732    3732 
   Size:       4028
 
BsaAI yAC'GTr

Cuts at:   1381    1381 
   Size:       4028
 
BsaBI GATnn'nnATC

Cuts at:      6    1542    3579       6 
   Size:       1536    2037     455

  Fragments arranged by size: 

                2037    1536     455
 
BsaHI Gr'CG_yC

Cuts at:    842    1459    1707    2409    3905     842 
   Size:        617     248     702    1496     965

  Fragments arranged by size: 

                1496     965     702     617     248
 
BsaWI w'CCGG_w

Cuts at:    188    1217    1738    2416    2690    2837    3248     188 
   Size:       1029     521     678     274     147     411     968

  Fragments arranged by size: 

                1029     968     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    481     511     904     934     481 
   Size:         30     393      30    3575

  Fragments arranged by size: 

                3575     393      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    279     925     279 
   Size:        646    3382
 
BseYI C'CCAG_C

Cuts at:   1453    2211    2788    3284    1453 
   Size:        758     577     496    2197

  Fragments arranged by size: 

                2197     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1570    1616    2824     104 
   Size:       1466      46    1208    1308

  Fragments arranged by size: 

                1466    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    320    1007    1042    1820    2010    2802     320 
   Size:        687      35     778     190     792    1546

  Fragments arranged by size: 

                1546     792     778     687     190      35
 
Bsp1286I G_dGCh'C

Cuts at:    320    1007    1042    1653    1746    1820    2010    2072    2802 
   Size:        687      35     611      93      74     190      62     730

Cuts at:   2802     320 
   Size:       1546

  Fragments arranged by size: 

                1546     730     687     611     190      93      74      62
                  35
 
BspEI T'CCGG_A

Cuts at:    188    3248     188 
   Size:       3060     968
 
BspHI T'CATG_A

Cuts at:    383    3204     383 
   Size:       2821    1207
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1078    1292    1594    1975    1078 
   Size:        214     302     381    3131

  Fragments arranged by size: 

                3131     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     393    2320    2374    3858    4023      50 
   Size:        343    1927      54    1484     165      55

  Fragments arranged by size: 

                1927    1484     343     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1940    1940 
   Size:       4028
 
BsrFI r'CCGG_y

Cuts at:   2026    2207    2416    2026 
   Size:        181     209    3638

  Fragments arranged by size: 

                3638     209     181
 
BssSI C'ACGA_G

Cuts at:   2299    2657    2299 
   Size:        358    3670
 
BstAPI GCAn_nnn'nTGC

Cuts at:    181    1405     181 
   Size:       1224    2804
 
BstBI TT'CG_AA

Cuts at:   2389    2389 
   Size:       4028
 
BstXI CCAn_nnnn'nTGG

Cuts at:    750     750 
   Size:       4028
 
BstYI r'GATC_y

Cuts at:      1     156     444     678    1053    1520    1543    1878    2124 
   Size:        155     288     234     375     467      23     335     246

Cuts at:   2124    3136    3222    3234    3580       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     375     346     335     288     246
                 234     155      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    558     558 
   Size:       4028
 
BtgI C'CryG_G

Cuts at:    107     530     919     107 
   Size:        423     389    3216

  Fragments arranged by size: 

                3216     423     389
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1013    1978    2159    2322    1013 
   Size:        965     181     163    2719

  Fragments arranged by size: 

                2719     965     181     163
 
BtsI GCAGTG_nn'

Cuts at:    182     305     932    1271     182 
   Size:        123     627     339    2939

  Fragments arranged by size: 

                2939     627     339     123
 
DraI TTT'AAA

Cuts at:   1027    1027 
   Size:       4028
 
DrdI GACnn_nn'nnGTC

Cuts at:   1734    2592    1734 
   Size:        858    3170
 
EaeI y'GGCC_r

Cuts at:    536     599    1613    1787    2178    2205    3550     536 
   Size:         63    1014     174     391      27    1345    1014

  Fragments arranged by size: 

                1345    1014    1014     391     174      63      27
 
EagI C'GGCC_G

Cuts at:   1613    1613 
   Size:       4028
 
EarI CTCTTCn'nnn_

Cuts at:    205     325    2051    2261     205 
   Size:        120    1726     210    1972

  Fragments arranged by size: 

                1972    1726     210     120
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2236    2546    2692    3838    2236 
   Size:        310     146    1146    2426

  Fragments arranged by size: 

                2426    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    318    1005    1040     318 
   Size:        687      35    3306

  Fragments arranged by size: 

                3306     687      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    220     483     719    1853    2285    3032    3300     220 
   Size:        263     236    1134     432     747     268     948

  Fragments arranged by size: 

                1134     948     747     432     268     263     236
 
EcoNI CCTnn'n_nnAGG

Cuts at:    907     907 
   Size:       4028
 
EcoO109I rG'GnC_Cy

Cuts at:   1167    1167 
   Size:       4028
 
EcoRI G'AATT_C

Cuts at:   1032    1032 
   Size:       4028
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    697    2047     697 
   Size:       1350    2678
 
FspI TGC'GCA

Cuts at:   1809    1809 
   Size:       4028
 
HaeII r_GCGC'y

Cuts at:   1462    1710    2732    3711    1462 
   Size:        248    1022     979    1779

  Fragments arranged by size: 

                1779    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    154     186     234     266     887     919    1870    1900    1902 
   Size:         32      48      32     621      32     951      30       2

Cuts at:   1902    1932     154 
   Size:         30    2250

  Fragments arranged by size: 

                2250     951     621      48      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    927    1067     927 
   Size:        140    3888
 
KasI G'GCGC_C

Cuts at:   1458    1706    1458 
   Size:        248    3780
 
KpnI G_GTAC'C

Cuts at:   1048    1048 
   Size:       4028
 
MluI A'CGCG_T

Cuts at:   3461    3461 
   Size:       4028
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2699    2883    2699 
   Size:        184    3844
 
MscI TGG'CCA

Cuts at:    601    1789     601 
   Size:       1188    2840
 
MslI CAynn'nnrTG

Cuts at:    637    2017    2144     637 
   Size:       1380     127    2521

  Fragments arranged by size: 

                2521    1380     127
 
MspA1I CmG'CkG

Cuts at:   1139    1453    1813    2826    3071    1139 
   Size:        314     360    1013     245    2096

  Fragments arranged by size: 

                2096    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2209    2209 
   Size:       4028
 
NarI GG'CG_CC

Cuts at:   1459    1707    1459 
   Size:        248    3780
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4028
 
NgoMIV G'CCGG_C

Cuts at:   2207    2207 
   Size:       4028
 
NspI r_CATG'y

Cuts at:   1081    1581    2488    1081 
   Size:        500     907    2621

  Fragments arranged by size: 

                2621     907     500
 
PciI A'CATG_T

Cuts at:   2484    2484 
   Size:       4028
 
PfoI T'CCnGG_A

Cuts at:    518     518 
   Size:       4028
 
PmeI GTTT'AAAC

Cuts at:   1027    1027 
   Size:       4028
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    392     424    3167    3199     392 
   Size:         32    2743      32    1221

  Fragments arranged by size: 

                2743    1221      32      32
 
PstI C_TGCA'G

Cuts at:   1075    1760    1075 
   Size:        685    3343
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4028
 
PvuII CAG'CTG

Cuts at:   1453    1813    1453 
   Size:        360    3668
 
RsrII CG'GwC_CG

Cuts at:   2223    2223 
   Size:       4028
 
SacI G_AGCT'C

Cuts at:    320    1007    1042     320 
   Size:        687      35    3306

  Fragments arranged by size: 

                3306     687      35
 
SalI G'TCGA_C

Cuts at:    925    1065     925 
   Size:        140    3888
 
SapI GCTCTTCn'nnn_

Cuts at:    325    2051    2261     325 
   Size:       1726     210    2092

  Fragments arranged by size: 

                2092    1726     210
 
SbfI CC_TGCA'GG

Cuts at:   1075    1075 
   Size:       4028
 
ScaI AGT'ACT

Cuts at:   3545    3545 
   Size:       4028
 
SfcI C'TryA_G

Cuts at:     33    1071    1756    2749    2940      33 
   Size:       1038     685     993     191    1121

  Fragments arranged by size: 

                1121    1038     993     685     191
 
SfoI GGC'GCC

Cuts at:   1460    1708    1460 
   Size:        248    3780
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4028
 
SmaI CCC'GGG

Cuts at:   1050    1050 
   Size:       4028
 
SmlI C'TyrA_G

Cuts at:    877    1183    2098    2590    2852     877 
   Size:        306     915     492     262    2053

  Fragments arranged by size: 

                2053     915     492     306     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4028
 
SphI G_CATG'C

Cuts at:   1081    1581    1081 
   Size:        500    3528
 
SspI AAT'ATT

Cuts at:    482     482 
   Size:       4028
 
StyI C'CwwG_G

Cuts at:    107    1162    1516     107 
   Size:       1055     354    2619

  Fragments arranged by size: 

                2619    1055     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1611    1744    2203    2412    1611 
   Size:        133     459     209    3227

  Fragments arranged by size: 

                3227     459     209     133
 
TatI w'GTAC_w

Cuts at:   3543    3543 
   Size:       4028
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    481     513     904     936    3545    3577     481 
   Size:         32     391      32    2609      32     932

  Fragments arranged by size: 

                2609     932     391      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1825    1825 
   Size:       4028
 
XbaI T'CTAG_A

Cuts at:   1059    1059 
   Size:       4028
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1523    1523 
   Size:       4028
 
XmaI C'CCGG_G

Cuts at:   1048    1048 
   Size:       4028
 
XmnI GAAnn'nnTTC

Cuts at:    404     404 
   Size:       4028
 
ZraI GAC'GTC

Cuts at:    843     843 
   Size:       4028
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PfoI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AloI     ApaI     AscI    AvrII
     BaeI     BbsI    BfrBI    BmgBI     BmtI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII   Bsu36I     ClaI    CspCI   DraIII
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI
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