Restriction map for pF1KB6460
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoO65I     EcoT14I     EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AcsI           52  GAATTC               [GA]^AATT[CT]
Aor51HI       839  AGCGCT               AGC^GCT
ApaI          668  GGGCCC               GGGCC^C
ApoI           52  GAATTC               [GA]^AATT[CT]
AvaI          319  CTCGAG               C^[CT]CG[GA]G
BanI          601  GGCACC               G^G[CT][GA]CC
BciVI         969  GGATAC               GTATCC(6/5)
BsaAI         367  TACGTG               [CT]AC^GT[GA]
BsaBI         909  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          472  CTGCAC               GTGCAG(16/14)
BsmBI         753  CGTCTC               CGTCTC(1/5)
BsoBI         319  CTCGAG               C^[CT]CG[GA]G
BsrBI         101  CCGCTC               CCGCTC(-3/-3)
DraI          955  TTTAAA               TTT^AAA
DsaI          656  CCGTGG               C^C[GA][CT]GG
EaeI          695  TGGCCG               [CT]^GGCC[GA]
Eco47III      839  AGCGCT               AGC^GCT
EcoNI         816  CCTCCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI          52  GAATTC               G^AATTC
EcoRV         871  GATATC               GAT^ATC
HaeII         839  AGCGCT               [GA]GCGC^[CT]
MaeII         368  ACGT                 A^CGT
MamI          909  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        698  CCGCTG               C[AC]G^C[GT]G
PaeR7I        319  CTCGAG               C^TCGAG
PstI          820  CTGCAG               CTGCA^G
SbfI          819  CCTGCAGG             CCTGCA^GG
Sse8387I      819  CCTGCAGG             CCTGCA^GG
Tsp45I        547  GTGAC                ^GT[GC]AC
Tsp509I        53  AATT                 ^AATT
TspRI         417  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI          319  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII         441  CGCG                 CG^CG
              571  CGCG                
AfaI          366  GTAC                 GT^AC
              612  GTAC                
Asp700I        48  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
               73  GAAGATTTTC          
BbvI           29  GCAGC                GCAGC(8/12)
              807  GCTGC               
BglII         737  AGATCT               A^GATCT
              828  AGATCT              
BsaI          193  GAGACC               GGTCTC(1/5)
             1011  GGTCTC              
BsmFI         497  GGGAC                GGGAC(10/14)
               98  GTCCC               
BspMI         803  ACCTGC               ACCTGC(4/8)
              822  GCAGGT              
BsrI          662  ACTGG                ACTGG(1/-1)
              905  ACTGG               
BstF5I        282  CATCC                GGATG(2/0)
              732  CATCC               
BstUI         441  CGCG                 CG^CG
              571  CGCG                
FokI          282  CATCC                GGATG(9/13)
              732  CATCC               
HincII        295  GTCAAC               GT[CT]^[GA]AC
              865  GTCAAC              
HindII        295  GTCAAC               GT[CT]^[GA]AC
              865  GTCAAC              
HinfI         137  GAATC                G^A[ACGT]TC
              513  GAATC               
MaeIII        547  GTGAC                ^GT[ACGT]AC
              977  GTAAC               
MseI           83  TTAA                 T^TAA
              956  TTAA                
MslI          354  CATGGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              540  CATTCAGGTG          
MvnI          441  CGCG                 CG^CG
              571  CGCG                
PpuMI         497  GGGACCT              [GA]G^G[AT]CC[CT]
              800  AGGACCT             
RsaI          366  GTAC                 GT^AC
              612  GTAC                
SfaNI         174  GATGC                GCATC(5/9)
              281  GCATC               
SfcI          492  CTACAG               C^T[GA][CT]AG
              820  CTGCAG              
SmlI          319  CTCGAG               C^T[CT][GA]AG
              853  CTCAAG              
TfiI          137  GAATC                G^A[AT]TC
              513  GAATC               
Tru9I          83  TTAA                 T^TAA
              956  TTAA                
TseI           29  GCAGC                G^C[AT]GC
              807  GCTGC               
XmnI           48  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
               73  GAAGATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanII       
BcnI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MboI        MboII       MflI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       TaqI        TthHB8I     XhoII       
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