Restriction map for pF1KB6449
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AviII       AvrII       BamHI       
BbeI        BbsI        BcgI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EarI        Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflMI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Van91I      XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          557  GTCTAC               GT^[AC][GT]AC
AfaI          123  GTAC                 GT^AC
ApaI           42  GGGCCC               GGGCC^C
Asp700I       823  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         412  GAGCTC               G[AT]GC[AT]^C
AspI          489  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          160  CTCGAG               C^[CT]CG[GA]G
AvaII         675  GGACC                G^G[AT]CC
BanI          174  GGTGCC               G^G[CT][GA]CC
BbrPI         552  CACGTG               CAC^GTG
BbvCI         379  GCTGAGG              CCTCAGC(-5/-2)
BciVI         791  GGATAC               GTATCC(6/5)
BclI          356  TGATCA               T^GATCA
BcnI          788  CCCGG                CC^[GC]GG
BfaI          820  CTAG                 C^TAG
BsaAI         552  CACGTG               [CT]AC^GT[GA]
BsaI          296  GAGACC               GGTCTC(1/5)
BsiHKAI       412  GAGCTC               G[AT]GC[AT]^C
BsmFI         221  GGGAC                GGGAC(10/14)
BsoBI         160  CTCGAG               C^[CT]CG[GA]G
BsrBI          67  GAGCGG               CCGCTC(-3/-3)
BsrI           75  ACTGG                ACTGG(1/-1)
BstAPI        168  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         152  AGATCC               [GA]^GATC[CT]
EagI           11  CGGCCG               C^GGCCG
EclXI          11  CGGCCG               C^GGCCG
Eco52I         11  CGGCCG               C^GGCCG
FbaI          356  TGATCA               T^GATCA
HaeII          17  AGCGCC               [GA]GCGC^[CT]
HgaI          939  GCGTC                GACGC(5/10)
HincII        727  GTCAAC               GT[CT]^[GA]AC
HindII        727  GTCAAC               GT[CT]^[GA]AC
MaeI          820  CTAG                 C^TAG
MflI          152  AGATCC               [GA]^GATC[CT]
MslI          270  CATGGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          788  CCCGG                CC^[GC]GG
PaeR7I        160  CTCGAG               C^TCGAG
PflFI         489  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         552  CACGTG               CAC^GTG
PmlI          552  CACGTG               CAC^GTG
RsaI          123  GTAC                 GT^AC
SacI          412  GAGCTC               GAGCT^C
SexAI         692  ACCTGGT              A^CC[AT]GGT
SmlI          160  CTCGAG               C^T[CT][GA]AG
Tsp45I        699  GTCAC                ^GT[GC]AC
Tsp509I       375  AATT                 ^AATT
Tth111I       489  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          160  CTCGAG               C^TCGAG
XhoII         152  AGATCC               [GA]^GATC[CT]
XmnI          823  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII        551  ACACGT               A^C[GA][CT]GT
              553  ACGTGT              
AhdI          691  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              868  GACACAGAGTC         
AspEI         691  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              868  GACACAGAGTC         
BseRI         118  GAGGAG               GAGGAG(10/8)
              856  GAGGAG              
BsgI          232  CTGCAC               GTGCAG(16/14)
              498  CTGCAC              
BsiEI          11  CGGCCG               CG[GA][CT]^CG
               81  CGACCG              
BsmAI         296  GAGAC                GTCTC(1/5)
              419  GAGAC               
BspMI         326  ACCTGC               ACCTGC(4/8)
              677  ACCTGC              
CfoI           18  GCGC                 GCG^C
              180  GCGC                
DraII          41  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               42  GGGCCCC             
Eam1105I      691  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              868  GACACAGAGTC         
EcoO109I       41  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               42  GGGCCCC             
FauI           47  CCCGC                CCCGC(4/6)
              507  CCCGC               
HapII         651  CCGG                 C^CGG
              789  CCGG                
HhaI           18  GCGC                 GCG^C
              180  GCGC                
HinP1I         18  GCGC                 G^CGC
              180  GCGC                
HpaII         651  CCGG                 C^CGG
              789  CCGG                
MaeII         553  ACGT                 A^CGT
              946  ACGT                
MaeIII        699  GTCAC                ^GT[ACGT]AC
              736  GTTAC               
MboII          60  GAAGA                GAAGA(8/7)
              253  GAAGA               
MspI          651  CCGG                 C^CGG
              789  CCGG                
PstI          145  CTGCAG               CTGCA^G
              328  CTGCAG              
PvuII         802  CAGCTG               CAG^CTG
              811  CAGCTG              
SfaNI         834  GATGC                GCATC(5/9)
              590  GCATC               
SfcI          145  CTGCAG               C^T[GA][CT]AG
              328  CTGCAG              
TaqI          161  TCGA                 T^CGA
              426  TCGA                
TspRI         263  CACTG                CA[GC]TG[ACGT][ACGT]^
              671  CAGTG               
TthHB8I       161  TCGA                 T^CGA
              426  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
BalI        BanII       BbvI        BmyI        BsaJI       
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       Cfr13I      DdeI        
DpnII       DsaI        EaeI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MboI        MluNI       MnlI        MscI        
MspA1I      MvaI        MvnI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       StyI        TseI        
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