Restriction map for pF1KB6449
 (Linear) (Six-base) MAPSORT of: KIBB6449.seq  Check: 4486  from: 1  to: 951



KIBB6449.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:      0     558     951 
   Size:        558     393
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     547     814     951 
   Size:         79     468     267     137

  Fragments arranged by size: 

                 468     267     137      79
 
AflIII A'CryG_T

Cuts at:      0     551     553     951 
   Size:        551       2     398

  Fragments arranged by size: 

                 551     398       2
 
AhdI GACnn_n'nnGTC

Cuts at:      0     696     873     951 
   Size:        696     177      78

  Fragments arranged by size: 

                 696     177      78
 
AlwNI CAG_nnn'CTG

Cuts at:      0     804     837     942     951 
   Size:        804      33     105       9

  Fragments arranged by size: 

                 804     105      33       9
 
ApaI G_GGCC'C

Cuts at:      0      46     951 
   Size:         46     905
 
AvaI C'yCGr_G

Cuts at:      0     160     951 
   Size:        160     791
 
BanI G'GyrC_C

Cuts at:      0     174     951 
   Size:        174     777
 
BanII G_rGCy'C

Cuts at:      0      46     194     416     641     951 
   Size:         46     148     222     225     310

  Fragments arranged by size: 

                 310     225     222     148      46
 
BbvCI CC'TCA_GC

Cuts at:      0     380     951 
   Size:        380     571
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     785     951 
   Size:        785     166
 
BclI T'GATC_A

Cuts at:      0     356     951 
   Size:        356     595
 
Bme1580I G_kGCm'C

Cuts at:      0      46     951 
   Size:         46     905
 
Bpu10I CC'TnA_GC

Cuts at:      0     380     410     632     951 
   Size:        380      30     222     319

  Fragments arranged by size: 

                 380     319     222      30
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     290     951 
   Size:        290     661
 
BsaAI yAC'GTr

Cuts at:      0     554     951 
   Size:        554     397
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     502     532     951 
   Size:        502      30     419

  Fragments arranged by size: 

                 502     419      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     133     871     951 
   Size:        133     738      80

  Fragments arranged by size: 

                 738     133      80
 
BseYI C'CCAG_C

Cuts at:      0     804     951 
   Size:        804     147
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     483     951 
   Size:        217     266     468

  Fragments arranged by size: 

                 468     266     217
 
BsiEI CG_ry'CG

Cuts at:      0      14      84     951 
   Size:         14      70     867

  Fragments arranged by size: 

                 867      70      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     416     951 
   Size:        416     535
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     194     416     641     951 
   Size:         46     148     222     225     310

  Fragments arranged by size: 

                 310     225     222     148      46
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     335     686     951 
   Size:        335     351     265

  Fragments arranged by size: 

                 351     335     265
 
BsrBI CCG'CTC

Cuts at:      0      69     951 
   Size:         69     882
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     174     951 
   Size:        174     777
 
BstYI r'GATC_y

Cuts at:      0     152     951 
   Size:        152     799
 
BtgI C'CryG_G

Cuts at:      0      35     269     576     951 
   Size:         35     234     307     375

  Fragments arranged by size: 

                 375     307     234      35
 
BtsI GCAGTG_nn'

Cuts at:      0     677     951 
   Size:        677     274
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     329     364     951 
   Size:        329      35     587

  Fragments arranged by size: 

                 587     329      35
 
EaeI y'GGCC_r

Cuts at:      0      11     239     470     546     648     951 
   Size:         11     228     231      76     102     303

  Fragments arranged by size: 

                 303     231     228     102      76      11
 
EagI C'GGCC_G

Cuts at:      0      11     951 
   Size:         11     940
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     145     271     951 
   Size:        145     126     680

  Fragments arranged by size: 

                 680     145     126
 
EcoICRI GAG'CTC

Cuts at:      0     414     951 
   Size:        414     537
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     547     814     951 
   Size:         79     468     267     137

  Fragments arranged by size: 

                 468     267     137      79
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42      43     951 
   Size:         42       1     908

  Fragments arranged by size: 

                 908      42       1
 
HaeII r_GCGC'y

Cuts at:      0      21     951 
   Size:         21     930
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     208     240     682     714     859     891     951 
   Size:        208      32     442      32     145      32      60

  Fragments arranged by size: 

                 442     208     145      60      32      32      32
 
HincII GTy'rAC

Cuts at:      0     729     951 
   Size:        729     222
 
MscI TGG'CCA

Cuts at:      0     241     472     548     951 
   Size:        241     231      76     403

  Fragments arranged by size: 

                 403     241     231      76
 
MslI CAynn'nnrTG

Cuts at:      0     274     951 
   Size:        274     677
 
MspA1I CmG'CkG

Cuts at:      0      95     804     813     951 
   Size:         95     709       9     138

  Fragments arranged by size: 

                 709     138      95       9
 
NcoI C'CATG_G

Cuts at:      0      35     269     576     951 
   Size:         35     234     307     375

  Fragments arranged by size: 

                 375     307     234      35
 
PmlI CAC'GTG

Cuts at:      0     554     951 
   Size:        554     397
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     865     897     951 
   Size:        865      32      54

  Fragments arranged by size: 

                 865      54      32
 
PspOMI G'GGCC_C

Cuts at:      0      42     951 
   Size:         42     909
 
PspXI vC'TCGA_Gb

Cuts at:      0     160     951 
   Size:        160     791
 
PstI C_TGCA'G

Cuts at:      0     149     332     951 
   Size:        149     183     619

  Fragments arranged by size: 

                 619     183     149
 
PvuII CAG'CTG

Cuts at:      0     804     813     951 
   Size:        804       9     138

  Fragments arranged by size: 

                 804     138       9
 
SacI G_AGCT'C

Cuts at:      0     416     951 
   Size:        416     535
 
SexAI A'CCwGG_T

Cuts at:      0     692     951 
   Size:        692     259
 
SfcI C'TryA_G

Cuts at:      0     145     328     951 
   Size:        145     183     623

  Fragments arranged by size: 

                 623     183     145
 
SmlI C'TyrA_G

Cuts at:      0     160     951 
   Size:        160     791
 
StyI C'CwwG_G

Cuts at:      0      35     269     576     611     951 
   Size:         35     234     307      35     340

  Fragments arranged by size: 

                 340     307     234      35      35
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     604     951 
   Size:        604     347
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     502     534     951 
   Size:        502      32     417

  Fragments arranged by size: 

                 502     417      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     492     951 
   Size:        492     459
 
XhoI C'TCGA_G

Cuts at:      0     160     951 
   Size:        160     791
 
XmnI GAAnn'nnTTC

Cuts at:      0     827     951 
   Size:        827     124
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI    AlwNI     ApaI     AvaI     BanI
    BanII    BbvCI    BciVI     BclI Bme1580I   Bpu10I     BsaI    BsaAI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI
    BsrBI   BstAPI    BstYI     BtgI     BtsI    CspCI     EaeI     EagI
     EciI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     MscI
     MslI   MspA1I     NcoI     PmlI     PpiI   PspOMI    PspXI     PstI
    PvuII     SacI    SexAI     SfcI     SmlI     StyI    TaqII     TstI
  Tth111I     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI     BcgI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaBI
    BsaHI    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI     EarI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI    PpuMI    PshAI     PsiI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     XbaI     XcmI     XmaI
     ZraI
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