Restriction map for pF1KB6442
 (Linear) (Six-base) MAPSORT of: KIBB6442.seq  Check: 9452  from: 1  to: 1038



KIBB6442.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     434    1038 
   Size:        434     604
 
AatII G_ACGT'C

Cuts at:      0     865    1038 
   Size:        865     173
 
Acc65I G'GTAC_C

Cuts at:      0     608    1038 
   Size:        608     430
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     142     313    1038 
   Size:         87      55     171     725

  Fragments arranged by size: 

                 725     171      87      55
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      61      93    1038 
   Size:         61      32     945

  Fragments arranged by size: 

                 945      61      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     638    1033    1038 
   Size:        638     395       5

  Fragments arranged by size: 

                 638     395       5
 
AvaI C'yCGr_G

Cuts at:      0     886    1038 
   Size:        886     152
 
AvrII C'CTAG_G

Cuts at:      0     108    1038 
   Size:        108     930
 
BanI G'GyrC_C

Cuts at:      0     608    1038 
   Size:        608     430
 
BanII G_rGCy'C

Cuts at:      0      59     373     401     728    1038 
   Size:         59     314      28     327     310

  Fragments arranged by size: 

                 327     314     310      59      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     210     466    1038 
   Size:        210     256     572

  Fragments arranged by size: 

                 572     256     210
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1030    1038 
   Size:       1030       8
 
BglII A'GATC_T

Cuts at:      0     239    1038 
   Size:        239     799
 
BlpI GC'TnA_GC

Cuts at:      0     763    1038 
   Size:        763     275
 
Bme1580I G_kGCm'C

Cuts at:      0     182    1038 
   Size:        182     856
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     943     979    1038 
   Size:        397     546      36      59

  Fragments arranged by size: 

                 546     397      59      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385    1038 
   Size:        385     653
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     934    1038 
   Size:        934     104
 
BsaHI Gr'CG_yC

Cuts at:      0     251     623     862    1038 
   Size:        251     372     239     176

  Fragments arranged by size: 

                 372     251     239     176
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      61      91     547     577    1038 
   Size:         61      30     456      30     461

  Fragments arranged by size: 

                 461     456      61      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     156     736     799    1038 
   Size:        156     580      63     239

  Fragments arranged by size: 

                 580     239     156      63
 
BseYI C'CCAG_C

Cuts at:      0     232     261     975    1038 
   Size:        232      29     714      63

  Fragments arranged by size: 

                 714     232      63      29
 
BsiHKAI G_wGCw'C

Cuts at:      0     728    1038 
   Size:        728     310
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     182     373     401     728    1038 
   Size:         59     123     191      28     327     310

  Fragments arranged by size: 

                 327     310     191     123      59      28
 
BspHI T'CATG_A

Cuts at:      0      65     452     782    1038 
   Size:         65     387     330     256

  Fragments arranged by size: 

                 387     330     256      65
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     434    1038 
   Size:        434     604
 
BstEII G'GTnAC_C

Cuts at:      0     828    1038 
   Size:        828     210
 
BstYI r'GATC_y

Cuts at:      0     239    1038 
   Size:        239     799
 
BtgI C'CryG_G

Cuts at:      0     655     791    1038 
   Size:        655     136     247

  Fragments arranged by size: 

                 655     247     136
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     161    1038 
   Size:        161     877
 
EarI CTCTTCn'nnn_

Cuts at:      0     325     340     826     922    1038 
   Size:        325      15     486      96     116

  Fragments arranged by size: 

                 486     325     116      96      15
 
EcoICRI GAG'CTC

Cuts at:      0     726    1038 
   Size:        726     312
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     142     313     397     943     979    1038 
   Size:         87      55     171      84     546      36      59

  Fragments arranged by size: 

                 546     171      87      84      59      55      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1023    1038 
   Size:       1023      15
 
HaeII r_GCGC'y

Cuts at:      0       7    1038 
   Size:          7    1031
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     528     547     560     579     862     894    1038 
   Size:        528      19      13      19     283      32     144

  Fragments arranged by size: 

                 528     283     144      32      19      19      13
 
KpnI G_GTAC'C

Cuts at:      0     612    1038 
   Size:        612     426
 
MfeI C'AATT_G

Cuts at:      0     839    1038 
   Size:        839     199
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     905    1038 
   Size:        905     133
 
MslI CAynn'nnrTG

Cuts at:      0     469    1038 
   Size:        469     569
 
MspA1I CmG'CkG

Cuts at:      0     635    1038 
   Size:        635     403
 
NcoI C'CATG_G

Cuts at:      0     791    1038 
   Size:        791     247
 
NspI r_CATG'y

Cuts at:      0     474    1038 
   Size:        474     564
 
PasI CC'CwG_GG

Cuts at:      0     387    1038 
   Size:        387     651
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1033    1038 
   Size:       1033       5
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93    1038 
   Size:         61      32     945

  Fragments arranged by size: 

                 945      61      32
 
PstI C_TGCA'G

Cuts at:      0     479     751    1038 
   Size:        479     272     287

  Fragments arranged by size: 

                 479     287     272
 
PvuII CAG'CTG

Cuts at:      0     635    1038 
   Size:        635     403
 
SacI G_AGCT'C

Cuts at:      0     728    1038 
   Size:        728     310
 
SapI GCTCTTCn'nnn_

Cuts at:      0     826     922    1038 
   Size:        826      96     116

  Fragments arranged by size: 

                 826     116      96
 
ScaI AGT'ACT

Cuts at:      0     418    1038 
   Size:        418     620
 
SexAI A'CCwGG_T

Cuts at:      0     611     989    1038 
   Size:        611     378      49

  Fragments arranged by size: 

                 611     378      49
 
SfcI C'TryA_G

Cuts at:      0     475     699     747     771     856    1038 
   Size:        475     224      48      24      85     182

  Fragments arranged by size: 

                 475     224     182      85      48      24
 
SmlI C'TyrA_G

Cuts at:      0     400    1038 
   Size:        400     638
 
StuI AGG'CCT

Cuts at:      0     952    1038 
   Size:        952      86
 
StyI C'CwwG_G

Cuts at:      0      51     108     393     791    1038 
   Size:         51      57     285     398     247

  Fragments arranged by size: 

                 398     285     247      57      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     726     784    1038 
   Size:        726      58     254

  Fragments arranged by size: 

                 726     254      58
 
TatI w'GTAC_w

Cuts at:      0     416    1038 
   Size:        416     622
 
Tth111I GACn'n_nGTC

Cuts at:      0     622    1038 
   Size:        622     416
 
XmnI GAAnn'nnTTC

Cuts at:      0      73    1038 
   Size:         73     965
 
ZraI GAC'GTC

Cuts at:      0     863    1038 
   Size:        863     175
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AloI    AlwNI     AvaI    AvrII
     BanI    BanII     BbsI     BglI    BglII     BlpI Bme1580I     BpmI
    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I
    BspHI    BspMI   BstEII    BstYI     BtgI    BtgZI     EarI  EcoICRI
  Eco57MI EcoO109I    HaeII    Hin4I     KpnI     MfeI     MmeI     MslI
   MspA1I     NcoI     NspI     PasI    PflMI     PpiI     PstI    PvuII
     SacI     SapI     ScaI    SexAI     SfcI     SmlI     StuI     StyI
    TaqII     TatI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaWI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI     XbaI     XcmI     XhoI     XmaI
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