Restriction map for
pF1KB6565
(Circular) (Six-base) MAPSORT of: pF1KB6565.seq Check: 1998 from: 1 to: 4235
pF1KB6565 4235 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 798 798
Size: 4235
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4235
Acc65I G'GTAC_C
Cuts at: 497 1251 497
Size: 754 3481
AclI AA'CG_TT
Cuts at: 4075 4075
Size: 4235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 727 889 2060 2492 3239 3507 607
Size: 120 162 1171 432 747 268 1335
Fragments arranged by size:
1335 1171 747 432 268 162 120
AfeI AGC'GCT
Cuts at: 925 925
Size: 4235
AflIII A'CryG_T
Cuts at: 1587 2691 3668 1587
Size: 1104 977 2154
Fragments arranged by size:
2154 1104 977
AgeI A'CCGG_T
Cuts at: 2623 2623
Size: 4235
AlwNI CAG_nnn'CTG
Cuts at: 1540 3107 1540
Size: 1567 2668
ApaI G_GGCC'C
Cuts at: 899 899
Size: 4235
ApaLI G'TGCA_C
Cuts at: 3005 3005
Size: 4235
ApoI r'AATT_y
Cuts at: 1239 3588 3844 1239
Size: 2349 256 1630
Fragments arranged by size:
2349 1630 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 240 986 1255 240
Size: 746 269 3220
Fragments arranged by size:
3220 746 269
BamHI G'GATC_C
Cuts at: 189 431 1260 189
Size: 242 829 3164
Fragments arranged by size:
3164 829 242
BanI G'GyrC_C
Cuts at: 181 497 843 1251 1665 1913 1948 181
Size: 316 346 408 414 248 35 2468
Fragments arranged by size:
2468 414 408 346 316 248 35
BanII G_rGCy'C
Cuts at: 485 620 899 1249 2279 485
Size: 135 279 350 1030 2441
Fragments arranged by size:
2441 1030 350 279 135
BbeI G_GCGC'C
Cuts at: 185 1669 1917 185
Size: 1484 248 2503
Fragments arranged by size:
2503 1484 248
BbsI GAAGACnn'nnnn_
Cuts at: 213 895 990 213
Size: 682 95 3458
Fragments arranged by size:
3458 682 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1068 1102 1068
Size: 34 4201
BciVI GTATCCnnnnn_n'
Cuts at: 2133 2894 4235 2133
Size: 761 1341 2133
Fragments arranged by size:
2133 1341 761
BclI T'GATC_A
Cuts at: 1755 1755
Size: 4235
BglII A'GATC_T
Cuts at: 837 1750 3787 837
Size: 913 2037 1285
Fragments arranged by size:
2037 1285 913
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4235
Bme1580I G_kGCm'C
Cuts at: 899 1860 1953 3009 899
Size: 961 93 1056 2125
Fragments arranged by size:
2125 1056 961 93
BmrI ACTGGGnnnn_n'
Cuts at: 207 1447 1528 1863 4007 207
Size: 1240 81 335 2144 435
Fragments arranged by size:
2144 1240 435 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 504 2602 504
Size: 2098 2137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 840 157
Size: 683 3552
Bpu10I CC'TnA_GC
Cuts at: 232 232
Size: 4235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 2290 2782 3080 1411
Size: 879 492 298 2566
Fragments arranged by size:
2566 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3939 3939
Size: 4235
BsaAI yAC'GTr
Cuts at: 1588 1588
Size: 4235
BsaBI GATnn'nnATC
Cuts at: 6 634 1749 3786 6
Size: 628 1115 2037 455
Fragments arranged by size:
2037 1115 628 455
BsaHI Gr'CG_yC
Cuts at: 182 218 296 344 977 1666 1914 2616 4112
Size: 36 78 48 633 689 248 702 1496
Cuts at: 4112 182
Size: 305
Fragments arranged by size:
1496 702 689 633 305 248 78 48
36
BsaWI w'CCGG_w
Cuts at: 251 917 1424 1945 2623 2897 3044 3455 251
Size: 666 507 521 678 274 147 411 1031
Fragments arranged by size:
1031 678 666 521 507 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 187 217 187
Size: 30 4205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 319
Size: 4235
BseYI C'CCAG_C
Cuts at: 1660 2418 2995 3491 1660
Size: 758 577 496 2404
Fragments arranged by size:
2404 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 154 1101 1777 1823 3031 104
Size: 50 947 676 46 1208 1308
Fragments arranged by size:
1308 1208 947 676 50 46
BsiHKAI G_wGCw'C
Cuts at: 585 644 1249 2027 2217 3009 585
Size: 59 605 778 190 792 1811
Fragments arranged by size:
1811 792 778 605 190 59
BsmI GAATG_Cn'
Cuts at: 814 814
Size: 4235
BsmBI CGTCTCn'nnnn_
Cuts at: 1117 1117
Size: 4235
Bsp1286I G_dGCh'C
Cuts at: 485 585 620 644 899 1249 1860 1953 2027
Size: 100 35 24 255 350 611 93 74
Cuts at: 2027 2217 2279 3009 485
Size: 190 62 730 1711
Fragments arranged by size:
1711 730 611 350 255 190 100 93
74 62 35 24
BspEI T'CCGG_A
Cuts at: 917 3455 917
Size: 2538 1697
BspHI T'CATG_A
Cuts at: 3411 3411
Size: 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 684 798 812 1285 1499 1801 2182 684
Size: 114 14 473 214 302 381 2737
Fragments arranged by size:
2737 473 381 302 214 114 14
BsrBI CCG'CTC
Cuts at: 50 120 2527 2581 4065 4230 50
Size: 70 2407 54 1484 165 55
Fragments arranged by size:
2407 1484 165 70 55 54
BsrDI GCAATG_nn'
Cuts at: 2147 2147
Size: 4235
BsrFI r'CCGG_y
Cuts at: 389 663 2233 2414 2623 389
Size: 274 1570 181 209 2001
Fragments arranged by size:
2001 1570 274 209 181
BssHII G'CGCG_C
Cuts at: 163 163
Size: 4235
BssSI C'ACGA_G
Cuts at: 2506 2864 2506
Size: 358 3877
BstAPI GCAn_nnn'nTGC
Cuts at: 1612 1612
Size: 4235
BstBI TT'CG_AA
Cuts at: 2596 2596
Size: 4235
BstEII G'GTnAC_C
Cuts at: 696 696
Size: 4235
BstYI r'GATC_y
Cuts at: 1 189 431 837 1260 1727 1750 2085 2331
Size: 188 242 406 423 467 23 335 246
Cuts at: 2331 3343 3429 3441 3787 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 423 406 346 335 246
242 188 86 23 12
BtgI C'CryG_G
Cuts at: 107 333 684 935 107
Size: 226 351 251 3407
Fragments arranged by size:
3407 351 251 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2185 2366 2529 2185
Size: 181 163 3891
Fragments arranged by size:
3891 181 163
BtsI GCAGTG_nn'
Cuts at: 433 1208 1478 433
Size: 775 270 3190
Fragments arranged by size:
3190 775 270
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 999 1034 999
Size: 35 4200
DraI TTT'AAA
Cuts at: 1234 1234
Size: 4235
DrdI GACnn_nn'nnGTC
Cuts at: 1941 2799 1941
Size: 858 3377
EaeI y'GGCC_r
Cuts at: 110 151 525 665 675 1069 1820 1994 2385
Size: 41 374 140 10 394 751 174 391
Cuts at: 2385 2412 3757 110
Size: 27 1345 588
Fragments arranged by size:
1345 751 588 394 391 374 174 140
41 27 10
EagI C'GGCC_G
Cuts at: 151 1820 151
Size: 1669 2566
EarI CTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4025
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 277 2443 2753 2899 4045 277
Size: 2166 310 146 1146 467
Fragments arranged by size:
2166 1146 467 310 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 607 727 840 889 2060 2492 3239 3507
Size: 450 120 113 49 1171 432 747 268
Cuts at: 3507 157
Size: 885
Fragments arranged by size:
1171 885 747 450 432 268 120 113
49
EcoO109I rG'GnC_Cy
Cuts at: 895 1374 895
Size: 479 3756
EcoRI G'AATT_C
Cuts at: 1239 1239
Size: 4235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 4235
FspI TGC'GCA
Cuts at: 2016 2016
Size: 4235
HaeII r_GCGC'y
Cuts at: 185 927 1669 1917 2939 3918 185
Size: 742 742 248 1022 979 502
Fragments arranged by size:
1022 979 742 742 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 980 1012 1114 1146 2077 2107 2109
Size: 32 761 32 102 32 931 30 2
Cuts at: 2109 2139 187
Size: 30 2283
Fragments arranged by size:
2283 931 761 102 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1274 1274
Size: 4235
KasI G'GCGC_C
Cuts at: 181 1665 1913 181
Size: 1484 248 2503
Fragments arranged by size:
2503 1484 248
KpnI G_GTAC'C
Cuts at: 501 1255 501
Size: 754 3481
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 428 1157 2906 3090 428
Size: 729 1749 184 1573
Fragments arranged by size:
1749 1573 729 184
MscI TGG'CCA
Cuts at: 112 1071 1996 112
Size: 959 925 2351
Fragments arranged by size:
2351 959 925
MslI CAynn'nnrTG
Cuts at: 2224 2351 2224
Size: 127 4108
MspA1I CmG'CkG
Cuts at: 150 1346 1660 2020 3033 3278 150
Size: 1196 314 360 1013 245 1107
Fragments arranged by size:
1196 1107 1013 360 314 245
NaeI GCC'GGC
Cuts at: 665 2416 665
Size: 1751 2484
NarI GG'CG_CC
Cuts at: 182 1666 1914 182
Size: 1484 248 2503
Fragments arranged by size:
2503 1484 248
NcoI C'CATG_G
Cuts at: 107 935 107
Size: 828 3407
NgoMIV G'CCGG_C
Cuts at: 663 2414 663
Size: 1751 2484
NspI r_CATG'y
Cuts at: 1288 1788 2695 1288
Size: 500 907 2828
Fragments arranged by size:
2828 907 500
PasI CC'CwG_GG
Cuts at: 820 1056 820
Size: 236 3999
PciI A'CATG_T
Cuts at: 2691 2691
Size: 4235
PfoI T'CCnGG_A
Cuts at: 134 856 134
Size: 722 3513
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 4203
PspOMI G'GGCC_C
Cuts at: 895 895
Size: 4235
PstI C_TGCA'G
Cuts at: 149 695 809 1282 1967 149
Size: 546 114 473 685 2417
Fragments arranged by size:
2417 685 546 473 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 1660 2020 1660
Size: 360 3875
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4235
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4025
SbfI CC_TGCA'GG
Cuts at: 695 809 1282 695
Size: 114 473 3648
Fragments arranged by size:
3648 473 114
ScaI AGT'ACT
Cuts at: 3752 3752
Size: 4235
SfcI C'TryA_G
Cuts at: 33 145 691 805 1278 1963 2956 3147 33
Size: 112 546 114 473 685 993 191 1121
Fragments arranged by size:
1121 993 685 546 473 191 114 112
SfoI GGC'GCC
Cuts at: 183 1667 1915 183
Size: 1484 248 2503
Fragments arranged by size:
2503 1484 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SmaI CCC'GGG
Cuts at: 1257 1257
Size: 4235
SmlI C'TyrA_G
Cuts at: 1390 2305 2797 3059 1390
Size: 915 492 262 2566
Fragments arranged by size:
2566 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 1288 1788 1288
Size: 500 3735
StyI C'CwwG_G
Cuts at: 107 113 611 620 935 1369 1723 107
Size: 6 498 9 315 434 354 2619
Fragments arranged by size:
2619 498 434 354 315 9 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1069 1137 1818 1951 2410 2619 1069
Size: 68 681 133 459 209 2685
Fragments arranged by size:
2685 681 459 209 133 68
TatI w'GTAC_w
Cuts at: 956 3750 956
Size: 2794 1441
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4203
Tth111I GACn'n_nGTC
Cuts at: 1128 2032 1128
Size: 904 3331
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1072 1730 1072
Size: 658 3577
XmaI C'CCGG_G
Cuts at: 1255 1255
Size: 4235
XmnI GAAnn'nnTTC
Cuts at: 592 592
Size: 4235
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsrGI BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI