Restriction map for pF1KB6349
 (Circular) (Six-base) MAPSORT of: pF1KB6349.seq  Check: 3709  from: 1  to: 3911



pF1KB6349  3911 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    718     718 
   Size:       3911
 
AccI GT'mk_AC

Cuts at:    949     949 
   Size:       3911
 
Acc65I G'GTAC_C

Cuts at:    927     927 
   Size:       3911
 
AclI AA'CG_TT

Cuts at:   3751    3751 
   Size:       3911
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     840     868    1736    2168    2915    3183     139 
   Size:        701      28     868     432     747     268     867

  Fragments arranged by size: 

                 868     867     747     701     432     268      28
 
AfeI AGC'GCT

Cuts at:    149     149 
   Size:       3911
 
AflIII A'CryG_T

Cuts at:   1263    2367    3344    1263 
   Size:       1104     977    1830

  Fragments arranged by size: 

                1830    1104     977
 
AgeI A'CCGG_T

Cuts at:   2299    2299 
   Size:       3911
 
AlwNI CAG_nnn'CTG

Cuts at:    519     666    1216    2783     519 
   Size:        147     550    1567    1647

  Fragments arranged by size: 

                1647    1567     550     147
 
ApaLI G'TGCA_C

Cuts at:   2681    2681 
   Size:       3911
 
ApoI r'AATT_y

Cuts at:    915    3264    3520     915 
   Size:       2349     256    1306

  Fragments arranged by size: 

                2349    1306     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3911
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3911
 
AvaI C'yCGr_G

Cuts at:    931     931 
   Size:       3911
 
BamHI G'GATC_C

Cuts at:    936     936 
   Size:       3911
 
BanI G'GyrC_C

Cuts at:    927    1341    1589    1624     927 
   Size:        414     248      35    3214

  Fragments arranged by size: 

                3214     414     248      35
 
BanII G_rGCy'C

Cuts at:    178     925    1955     178 
   Size:        747    1030    2134

  Fragments arranged by size: 

                2134    1030     747
 
BbeI G_GCGC'C

Cuts at:   1345    1593    1345 
   Size:        248    3663
 
BciVI GTATCCnnnnn_n'

Cuts at:   1809    2570    3911    1809 
   Size:        761    1341    1809

  Fragments arranged by size: 

                1809    1341     761
 
BclI T'GATC_A

Cuts at:   1431    1431 
   Size:       3911
 
BglII A'GATC_T

Cuts at:   1426    3463    1426 
   Size:       2037    1874
 
BlpI GC'TnA_GC

Cuts at:    172    1023     172 
   Size:        851    3060
 
Bme1580I G_kGCm'C

Cuts at:   1536    1629    2685    1536 
   Size:         93    1056    2762

  Fragments arranged by size: 

                2762    1056      93
 
BmgBI CAC'GTC

Cuts at:    324     324 
   Size:       3911
 
BmrI ACTGGGnnnn_n'

Cuts at:   1123    1204    1539    3683    1123 
   Size:         81     335    2144    1351

  Fragments arranged by size: 

                2144    1351     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2278    2278 
   Size:       3911
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    149     418     149 
   Size:        269    3642
 
Bpu10I CC'TnA_GC

Cuts at:    901     901 
   Size:       3911
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1087    1966    2458    2756    1087 
   Size:        879     492     298    2242

  Fragments arranged by size: 

                2242     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3615    3615 
   Size:       3911
 
BsaAI yAC'GTr

Cuts at:   1264    1264 
   Size:       3911
 
BsaBI GATnn'nnATC

Cuts at:      6    1425    3462       6 
   Size:       1419    2037     455

  Fragments arranged by size: 

                2037    1419     455
 
BsaHI Gr'CG_yC

Cuts at:   1342    1590    2292    3788    1342 
   Size:        248     702    1496    1465

  Fragments arranged by size: 

                1496    1465     702     248
 
BsaWI w'CCGG_w

Cuts at:   1100    1621    2299    2573    2720    3131    1100 
   Size:        521     678     274     147     411    1880

  Fragments arranged by size: 

                1880     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    313     313 
   Size:       3911
 
BseYI C'CCAG_C

Cuts at:    351     512     613     816    1336    2094    2671    3167     351 
   Size:        161     101     203     520     758     577     496    1095

  Fragments arranged by size: 

                1095     758     577     520     496     203     161     101
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    487     761     487 
   Size:        274    3637
 
BsiEI CG_ry'CG

Cuts at:    104    1453    1499    2707     104 
   Size:       1349      46    1208    1308

  Fragments arranged by size: 

                1349    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    178     725     765     925    1703    1893    2685     178 
   Size:        547      40     160     778     190     792    1404

  Fragments arranged by size: 

                1404     792     778     547     190     160      40
 
Bsp1286I G_dGCh'C

Cuts at:    178     725     765     925    1536    1629    1703    1893    1955 
   Size:        547      40     160     611      93      74     190      62

Cuts at:   1955    2685     178 
   Size:        730    1404

  Fragments arranged by size: 

                1404     730     611     547     190     160      93      74
                  62      40
 
BspEI T'CCGG_A

Cuts at:   3131    3131 
   Size:       3911
 
BspHI T'CATG_A

Cuts at:   3087    3087 
   Size:       3911
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    718     961    1175    1477    1858     718 
   Size:        243     214     302     381    2771

  Fragments arranged by size: 

                2771     381     302     243     214
 
BsrBI CCG'CTC

Cuts at:     50     261    2203    2257    3741    3906      50 
   Size:        211    1942      54    1484     165      55

  Fragments arranged by size: 

                1942    1484     211     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1823    1823 
   Size:       3911
 
BsrFI r'CCGG_y

Cuts at:   1909    2090    2299    1909 
   Size:        181     209    3521

  Fragments arranged by size: 

                3521     209     181
 
BssSI C'ACGA_G

Cuts at:   2182    2540    2182 
   Size:        358    3553
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1288    1288 
   Size:       3911
 
BstBI TT'CG_AA

Cuts at:   2272    2272 
   Size:       3911
 
BstYI r'GATC_y

Cuts at:      1     936    1403    1426    1761    2007    3019    3105    3117 
   Size:        935     467      23     335     246    1012      86      12

Cuts at:   3117    3463       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     935     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    485     485 
   Size:       3911
 
BtgI C'CryG_G

Cuts at:    107     413     107 
   Size:        306    3605
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1861    2042    2205    1861 
   Size:        181     163    3567

  Fragments arranged by size: 

                3567     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1154    1154 
   Size:       3911
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    170     205     170 
   Size:         35    3876
 
DraI TTT'AAA

Cuts at:    910     910 
   Size:       3911
 
DrdI GACnn_nn'nnGTC

Cuts at:   1617    2475    1617 
   Size:        858    3053
 
EaeI y'GGCC_r

Cuts at:    597    1496    1670    2061    2088    3433     597 
   Size:        899     174     391      27    1345    1075

  Fragments arranged by size: 

                1345    1075     899     391     174      27
 
EagI C'GGCC_G

Cuts at:   1496    1496 
   Size:       3911
 
EarI CTCTTCn'nnn_

Cuts at:    599     832    1934    2144     599 
   Size:        233    1102     210    2366

  Fragments arranged by size: 

                2366    1102     233     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2119    2429    2575    3721    2119 
   Size:        310     146    1146    2309

  Fragments arranged by size: 

                2309    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    176     923     176 
   Size:        747    3164
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     149     418     840     868    1736    2168    2915    3183 
   Size:         10     269     422      28     868     432     747     268

Cuts at:   3183     139 
   Size:        867

  Fragments arranged by size: 

                 868     867     747     432     422     269     268      28
                  10
 
EcoNI CCTnn'n_nnAGG

Cuts at:    400     400 
   Size:       3911
 
EcoO109I rG'GnC_Cy

Cuts at:   1050    1050 
   Size:       3911
 
EcoRI G'AATT_C

Cuts at:    915     915 
   Size:       3911
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1930    1930 
   Size:       3911
 
FspI TGC'GCA

Cuts at:   1692    1692 
   Size:       3911
 
HaeII r_GCGC'y

Cuts at:    151    1345    1593    2615    3594     151 
   Size:       1194     248    1022     979     468

  Fragments arranged by size: 

                1194    1022     979     468     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    222     254     483     515     726     758    1753    1783    1785 
   Size:         32     229      32     211      32     995      30       2

Cuts at:   1785    1815     222 
   Size:         30    2318

  Fragments arranged by size: 

                2318     995     229     211      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    950     950 
   Size:       3911
 
KasI G'GCGC_C

Cuts at:   1341    1589    1341 
   Size:        248    3663
 
KpnI G_GTAC'C

Cuts at:    931     931 
   Size:       3911
 
MluI A'CGCG_T

Cuts at:   3344    3344 
   Size:       3911
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    504    2582    2766     504 
   Size:       2078     184    1649

  Fragments arranged by size: 

                2078    1649     184
 
MscI TGG'CCA

Cuts at:   1672    1672 
   Size:       3911
 
MslI CAynn'nnrTG

Cuts at:   1900    2027    1900 
   Size:        127    3784
 
MspA1I CmG'CkG

Cuts at:    415     471    1022    1336    1696    2709    2954     415 
   Size:         56     551     314     360    1013     245    1372

  Fragments arranged by size: 

                1372    1013     551     360     314     245      56
 
NaeI GCC'GGC

Cuts at:   2092    2092 
   Size:       3911
 
NarI GG'CG_CC

Cuts at:   1342    1590    1342 
   Size:        248    3663
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3911
 
NgoMIV G'CCGG_C

Cuts at:   2090    2090 
   Size:       3911
 
NspI r_CATG'y

Cuts at:    964    1464    2371     964 
   Size:        500     907    2504

  Fragments arranged by size: 

                2504     907     500
 
PciI A'CATG_T

Cuts at:   2367    2367 
   Size:       3911
 
PflMI CCAn_nnn'nTGG

Cuts at:    666     666 
   Size:       3911
 
PfoI T'CCnGG_A

Cuts at:    272     395     272 
   Size:        123    3788
 
PmeI GTTT'AAAC

Cuts at:    910     910 
   Size:       3911
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3050    3082    3050 
   Size:         32    3879
 
PstI C_TGCA'G

Cuts at:    433     524     958    1643     433 
   Size:         91     434     685    2701

  Fragments arranged by size: 

                2701     685     434      91
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3911
 
PvuII CAG'CTG

Cuts at:   1336    1696    1336 
   Size:        360    3551
 
RsrII CG'GwC_CG

Cuts at:   2106    2106 
   Size:       3911
 
SacI G_AGCT'C

Cuts at:    178     925     178 
   Size:        747    3164
 
SacII CC_GC'GG

Cuts at:    416     416 
   Size:       3911
 
SalI G'TCGA_C

Cuts at:    948     948 
   Size:       3911
 
SapI GCTCTTCn'nnn_

Cuts at:    832    1934    2144     832 
   Size:       1102     210    2599

  Fragments arranged by size: 

                2599    1102     210
 
SbfI CC_TGCA'GG

Cuts at:    958     958 
   Size:       3911
 
ScaI AGT'ACT

Cuts at:    373    3428     373 
   Size:       3055     856
 
SfcI C'TryA_G

Cuts at:     33     429     520     954    1639    2632    2823      33 
   Size:        396      91     434     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     434     396     191      91
 
SfoI GGC'GCC

Cuts at:   1343    1591    1343 
   Size:        248    3663
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3911
 
SmaI CCC'GGG

Cuts at:    933     933 
   Size:       3911
 
SmlI C'TyrA_G

Cuts at:   1066    1981    2473    2735    1066 
   Size:        915     492     262    2242

  Fragments arranged by size: 

                2242     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3911
 
SphI G_CATG'C

Cuts at:    964    1464     964 
   Size:        500    3411
 
StuI AGG'CCT

Cuts at:    632     632 
   Size:       3911
 
StyI C'CwwG_G

Cuts at:    107     831    1045    1399     107 
   Size:        724     214     354    2619

  Fragments arranged by size: 

                2619     724     354     214
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    740    1494    1627    2086    2295     740 
   Size:        754     133     459     209    2356

  Fragments arranged by size: 

                2356     754     459     209     133
 
TatI w'GTAC_w

Cuts at:    371    3426     371 
   Size:       3055     856
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3428    3460    3428 
   Size:         32    3879
 
Tth111I GACn'n_nGTC

Cuts at:   1708    1708 
   Size:       3911
 
XbaI T'CTAG_A

Cuts at:    942     942 
   Size:       3911
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    215    1406     215 
   Size:       1191    2720
 
XmaI C'CCGG_G

Cuts at:    931     931 
   Size:       3911
 
XmnI GAAnn'nnTTC

Cuts at:    683     683 
   Size:       3911
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI     BmtI
    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I     ClaI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI
     ZraI
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