Restriction map for pF1KB6349
 (Linear) (Six-base) MAPSORT of: KIBB6349.seq  Check: 4223  from: 1  to: 798



KIBB6349.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     610     798 
   Size:        610     188
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     732     760     798 
   Size:         31     701      28      38

  Fragments arranged by size: 

                 701      38      31      28
 
AfeI AGC'GCT

Cuts at:      0      41     798 
   Size:         41     757
 
AlwNI CAG_nnn'CTG

Cuts at:      0     411     558     798 
   Size:        411     147     240

  Fragments arranged by size: 

                 411     240     147
 
BanII G_rGCy'C

Cuts at:      0      70     798 
   Size:         70     728
 
BlpI GC'TnA_GC

Cuts at:      0      64     798 
   Size:         64     734
 
BmgBI CAC'GTC

Cuts at:      0     216     798 
   Size:        216     582
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     310     798 
   Size:         41     269     488

  Fragments arranged by size: 

                 488     269      41
 
Bpu10I CC'TnA_GC

Cuts at:      0     793     798 
   Size:        793       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205     798 
   Size:        205     593
 
BseYI C'CCAG_C

Cuts at:      0     243     404     505     708     798 
   Size:        243     161     101     203      90

  Fragments arranged by size: 

                 243     203     161     101      90
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     653     798 
   Size:        379     274     145

  Fragments arranged by size: 

                 379     274     145
 
BsiHKAI G_wGCw'C

Cuts at:      0      70     617     657     798 
   Size:         70     547      40     141

  Fragments arranged by size: 

                 547     141      70      40
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     617     657     798 
   Size:         70     547      40     141

  Fragments arranged by size: 

                 547     141      70      40
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     610     798 
   Size:        610     188
 
BsrBI CCG'CTC

Cuts at:      0     153     798 
   Size:        153     645
 
Bsu36I CC'TnA_GG

Cuts at:      0     377     798 
   Size:        377     421
 
BtgI C'CryG_G

Cuts at:      0     305     798 
   Size:        305     493
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      62      97     798 
   Size:         62      35     701

  Fragments arranged by size: 

                 701      62      35
 
EaeI y'GGCC_r

Cuts at:      0     489     798 
   Size:        489     309
 
EarI CTCTTCn'nnn_

Cuts at:      0     491     724     798 
   Size:        491     233      74

  Fragments arranged by size: 

                 491     233      74
 
EcoICRI GAG'CTC

Cuts at:      0      68     798 
   Size:         68     730
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31      41     310     732     760     798 
   Size:         31      10     269     422      28      38

  Fragments arranged by size: 

                 422     269      38      31      28      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     292     798 
   Size:        292     506
 
HaeII r_GCGC'y

Cuts at:      0      43     798 
   Size:         43     755
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     114     146     375     407     618     650     798 
   Size:        114      32     229      32     211      32     148

  Fragments arranged by size: 

                 229     211     148     114      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     396     798 
   Size:        396     402
 
MspA1I CmG'CkG

Cuts at:      0     307     363     798 
   Size:        307      56     435

  Fragments arranged by size: 

                 435     307      56
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     558     798 
   Size:        558     240
 
PfoI T'CCnGG_A

Cuts at:      0     164     287     798 
   Size:        164     123     511

  Fragments arranged by size: 

                 511     164     123
 
PstI C_TGCA'G

Cuts at:      0     325     416     798 
   Size:        325      91     382

  Fragments arranged by size: 

                 382     325      91
 
SacI G_AGCT'C

Cuts at:      0      70     798 
   Size:         70     728
 
SacII CC_GC'GG

Cuts at:      0     308     798 
   Size:        308     490
 
SapI GCTCTTCn'nnn_

Cuts at:      0     724     798 
   Size:        724      74
 
ScaI AGT'ACT

Cuts at:      0     265     798 
   Size:        265     533
 
SfcI C'TryA_G

Cuts at:      0     321     412     798 
   Size:        321      91     386

  Fragments arranged by size: 

                 386     321      91
 
StuI AGG'CCT

Cuts at:      0     524     798 
   Size:        524     274
 
StyI C'CwwG_G

Cuts at:      0     723     798 
   Size:        723      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     632     798 
   Size:        632     166
 
TatI w'GTAC_w

Cuts at:      0     263     798 
   Size:        263     535
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     107     798 
   Size:        107     691
 
XmnI GAAnn'nnTTC

Cuts at:      0     575     798 
   Size:        575     223
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI    BanII     BlpI    BmgBI     BpmI
   Bpu10I    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI
   Bsu36I     BtgI    CspCI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
    HaeII    Hin4I     MmeI   MspA1I    PflMI     PfoI     PstI     SacI
    SacII     SapI     ScaI     SfcI     StuI     StyI    TaqII     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EagI     EciI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SalI    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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