Restriction map for
pF1KB6347
(Linear) (Six-base) MAPSORT of: KIBB6347.seq Check: 7209 from: 1 to: 798
KIBB6347.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 732 760 798
Size: 31 701 28 38
Fragments arranged by size:
701 38 31 28
AflII C'TTAA_G
Cuts at: 0 118 798
Size: 118 680
AlwNI CAG_nnn'CTG
Cuts at: 0 411 558 798
Size: 411 147 240
Fragments arranged by size:
411 240 147
BanII G_rGCy'C
Cuts at: 0 70 798
Size: 70 728
BbsI GAAGACnn'nnnn_
Cuts at: 0 508 798
Size: 508 290
BciVI GTATCCnnnnn_n'
Cuts at: 0 401 798
Size: 401 397
BlpI GC'TnA_GC
Cuts at: 0 64 798
Size: 64 734
BmgBI CAC'GTC
Cuts at: 0 216 798
Size: 216 582
BmrI ACTGGGnnnn_n'
Cuts at: 0 515 798
Size: 515 283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 41 187 310 798
Size: 12 29 146 123 488
Fragments arranged by size:
488 146 123 29 12
Bpu10I CC'TnA_GC
Cuts at: 0 793 798
Size: 793 5
BsaWI w'CCGG_w
Cuts at: 0 482 798
Size: 482 316
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 798
Size: 205 593
BseYI C'CCAG_C
Cuts at: 0 85 243 404 708 798
Size: 85 158 161 304 90
Fragments arranged by size:
304 161 158 90 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 653 798
Size: 379 274 145
Fragments arranged by size:
379 274 145
BsiHKAI G_wGCw'C
Cuts at: 0 70 617 657 798
Size: 70 547 40 141
Fragments arranged by size:
547 141 70 40
Bsp1286I G_dGCh'C
Cuts at: 0 70 617 657 798
Size: 70 547 40 141
Fragments arranged by size:
547 141 70 40
BspEI T'CCGG_A
Cuts at: 0 482 798
Size: 482 316
BsrBI CCG'CTC
Cuts at: 0 153 798
Size: 153 645
Bsu36I CC'TnA_GG
Cuts at: 0 377 798
Size: 377 421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 89 124 798
Size: 89 35 674
Fragments arranged by size:
674 89 35
EaeI y'GGCC_r
Cuts at: 0 489 798
Size: 489 309
EarI CTCTTCn'nnn_
Cuts at: 0 491 724 798
Size: 491 233 74
Fragments arranged by size:
491 233 74
EcoICRI GAG'CTC
Cuts at: 0 68 798
Size: 68 730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 31 41 187 310 732 760 798
Size: 12 19 10 146 123 422 28 38
Fragments arranged by size:
422 146 123 38 28 19 12 10
EcoO109I rG'GnC_Cy
Cuts at: 0 282 798
Size: 282 516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 375 407 798
Size: 375 32 391
Fragments arranged by size:
391 375 32
HincII GTy'rAC
Cuts at: 0 44 798
Size: 44 754
MscI TGG'CCA
Cuts at: 0 491 798
Size: 491 307
MspA1I CmG'CkG
Cuts at: 0 363 798
Size: 363 435
PflMI CCAn_nnn'nTGG
Cuts at: 0 558 798
Size: 558 240
PfoI T'CCnGG_A
Cuts at: 0 164 265 287 798
Size: 164 101 22 511
Fragments arranged by size:
511 164 101 22
PpuMI rG'GwC_Cy
Cuts at: 0 282 798
Size: 282 516
PstI C_TGCA'G
Cuts at: 0 325 416 798
Size: 325 91 382
Fragments arranged by size:
382 325 91
SacI G_AGCT'C
Cuts at: 0 70 798
Size: 70 728
SapI GCTCTTCn'nnn_
Cuts at: 0 724 798
Size: 724 74
SfcI C'TryA_G
Cuts at: 0 321 412 798
Size: 321 91 386
Fragments arranged by size:
386 321 91
SmlI C'TyrA_G
Cuts at: 0 118 798
Size: 118 680
StuI AGG'CCT
Cuts at: 0 524 798
Size: 524 274
StyI C'CwwG_G
Cuts at: 0 723 798
Size: 723 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 632 798
Size: 632 166
XmnI GAAnn'nnTTC
Cuts at: 0 575 798
Size: 575 223
Enzymes that do cut:
AcuI AflII AlwNI BanII BbsI BciVI BlpI BmgBI
BmrI BpmI Bpu10I BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BsrBI Bsu36I CspCI EaeI EarI EcoICRI
Eco57MI EcoO109I Hin4I HincII MscI MspA1I PflMI PfoI
PpuMI PstI SacI SapI SfcI SmlI StuI StyI
TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BclI BfrBI BglI BglII Bme1580I BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI
BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI