Restriction map for pF1KB6338
 (Linear) (Six-base) MAPSORT of: KIBB6338.seq  Check: 8218  from: 1  to: 870



KIBB6338.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:      0     350     870 
   Size:        350     520
 
Acc65I G'GTAC_C

Cuts at:      0     725     870 
   Size:        725     145
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     262     638     870 
   Size:        188      74     376     232

  Fragments arranged by size: 

                 376     232     188      74
 
AleI CACnn'nnGTG

Cuts at:      0      96     870 
   Size:         96     774
 
AlwNI CAG_nnn'CTG

Cuts at:      0     661     870 
   Size:        661     209
 
ApoI r'AATT_y

Cuts at:      0     838     870 
   Size:        838      32
 
AvaI C'yCGr_G

Cuts at:      0     220     321     870 
   Size:        220     101     549

  Fragments arranged by size: 

                 549     220     101
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     709     742     870 
   Size:        709      33     128

  Fragments arranged by size: 

                 709     128      33
 
BanI G'GyrC_C

Cuts at:      0     371     725     870 
   Size:        371     354     145

  Fragments arranged by size: 

                 371     354     145
 
BanII G_rGCy'C

Cuts at:      0      68     870 
   Size:         68     802
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     476     644     802     870 
   Size:        476     168     158      68

  Fragments arranged by size: 

                 476     168     158      68
 
BglII A'GATC_T

Cuts at:      0     171     870 
   Size:        171     699
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     217     424     870 
   Size:         79     138     207     446

  Fragments arranged by size: 

                 446     207     138      79
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     665     845     870 
   Size:        294     371     180      25

  Fragments arranged by size: 

                 371     294     180      25
 
BsaWI w'CCGG_w

Cuts at:      0      68     870 
   Size:         68     802
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     595     625     870 
   Size:        595      30     245

  Fragments arranged by size: 

                 595     245      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     870 
   Size:        112     758
 
BseYI C'CCAG_C

Cuts at:      0      13     870 
   Size:         13     857
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     870 
   Size:        427     443
 
BsiHKAI G_wGCw'C

Cuts at:      0     221     870 
   Size:        221     649
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     221     870 
   Size:         68     153     649

  Fragments arranged by size: 

                 649     153      68
 
BspEI T'CCGG_A

Cuts at:      0      68     870 
   Size:         68     802
 
BstBI TT'CG_AA

Cuts at:      0     807     870 
   Size:        807      63
 
BstYI r'GATC_y

Cuts at:      0     171     870 
   Size:        171     699
 
Bsu36I CC'TnA_GG

Cuts at:      0     266     484     870 
   Size:        266     218     386

  Fragments arranged by size: 

                 386     266     218
 
BtsI GCAGTG_nn'

Cuts at:      0     506     698     773     870 
   Size:        506     192      75      97

  Fragments arranged by size: 

                 506     192      97      75
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     167     202     870 
   Size:        167      35     668

  Fragments arranged by size: 

                 668     167      35
 
DraI TTT'AAA

Cuts at:      0     843     870 
   Size:        843      27
 
DraIII CAC_nnn'GTG

Cuts at:      0     284     870 
   Size:        284     586
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     188     217     262     424     638     870 
   Size:         79     109      29      45     162     214     232

  Fragments arranged by size: 

                 232     214     162     109      79      45      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0     444     855     870 
   Size:        444     411      15

  Fragments arranged by size: 

                 444     411      15
 
EcoRV GAT'ATC

Cuts at:      0      74     870 
   Size:         74     796
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     156     188     595     627     870 
   Size:        156      32     407      32     243

  Fragments arranged by size: 

                 407     243     156      32      32
 
HincII GTy'rAC

Cuts at:      0     351     698     870 
   Size:        351     347     172

  Fragments arranged by size: 

                 351     347     172
 
KpnI G_GTAC'C

Cuts at:      0     729     870 
   Size:        729     141
 
MslI CAynn'nnrTG

Cuts at:      0      96     870 
   Size:         96     774
 
MspA1I CmG'CkG

Cuts at:      0     117     154     497     608     870 
   Size:        117      37     343     111     262

  Fragments arranged by size: 

                 343     262     117     111      37
 
NspI r_CATG'y

Cuts at:      0     162     870 
   Size:        162     708
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     284     870 
   Size:        284     586
 
PfoI T'CCnGG_A

Cuts at:      0     194     870 
   Size:        194     676
 
PpuMI rG'GwC_Cy

Cuts at:      0     855     870 
   Size:        855      15
 
PspXI vC'TCGA_Gb

Cuts at:      0     321     870 
   Size:        321     549
 
PstI C_TGCA'G

Cuts at:      0     625     870 
   Size:        625     245
 
PvuII CAG'CTG

Cuts at:      0     117     154     497     870 
   Size:        117      37     343     373

  Fragments arranged by size: 

                 373     343     117      37
 
SalI G'TCGA_C

Cuts at:      0     349     870 
   Size:        349     521
 
SfcI C'TryA_G

Cuts at:      0     621     870 
   Size:        621     249
 
SmlI C'TyrA_G

Cuts at:      0     220     309     321     680     860     870 
   Size:        220      89      12     359     180      10

  Fragments arranged by size: 

                 359     220     180      89      12      10
 
SphI G_CATG'C

Cuts at:      0     162     870 
   Size:        162     708
 
StuI AGG'CCT

Cuts at:      0     319     870 
   Size:        319     551
 
SwaI ATTT'AAAT

Cuts at:      0     843     870 
   Size:        843      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     444     870 
   Size:        444     426
 
TatI w'GTAC_w

Cuts at:      0     365     870 
   Size:        365     505
 
XhoI C'TCGA_G

Cuts at:      0     220     321     870 
   Size:        220     101     549

  Fragments arranged by size: 

                 549     220     101
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AleI    AlwNI     ApoI     AvaI     BaeI
     BanI    BanII     BbsI    BglII     BpmI    BpuEI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BstBI    BstYI
   Bsu36I     BtsI    CspCI     DraI   DraIII  Eco57MI EcoO109I    EcoRV
    Hin4I   HincII     KpnI     MslI   MspA1I     NspI    PflMI     PfoI
    PpuMI    PspXI     PstI    PvuII     SalI     SfcI     SmlI     SphI
     StuI     SwaI    TaqII     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI     PsrI     PvuI    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StyI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI
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