Restriction map for
pF1KB6332
(Linear) (Six-base) MAPSORT of: KIBB6332.seq Check: 9555 from: 1 to: 864
KIBB6332.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 864
Size: 695 169
AlwNI CAG_nnn'CTG
Cuts at: 0 694 719 732 864
Size: 694 25 13 132
Fragments arranged by size:
694 132 25 13
ApoI r'AATT_y
Cuts at: 0 788 864
Size: 788 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 101 683 864
Size: 101 582 181
Fragments arranged by size:
582 181 101
BfrBI ATG'CAT
Cuts at: 0 28 102 750 864
Size: 28 74 648 114
Fragments arranged by size:
648 114 74 28
BglI GCCn_nnn'nGGC
Cuts at: 0 250 615 864
Size: 250 365 249
Fragments arranged by size:
365 250 249
Bme1580I G_kGCm'C
Cuts at: 0 853 864
Size: 853 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 676 864
Size: 676 188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 541 864
Size: 449 92 323
Fragments arranged by size:
449 323 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 864
Size: 627 237
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 788 791 839 864
Size: 472 316 3 48 25
Fragments arranged by size:
472 316 48 25 3
BseYI C'CCAG_C
Cuts at: 0 725 783 864
Size: 725 58 81
Fragments arranged by size:
725 81 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 680 851 864
Size: 142 538 171 13
Fragments arranged by size:
538 171 142 13
BsmI GAATG_Cn'
Cuts at: 0 28 864
Size: 28 836
Bsp1286I G_dGCh'C
Cuts at: 0 853 864
Size: 853 11
BtgI C'CryG_G
Cuts at: 0 658 864
Size: 658 206
DraIII CAC_nnn'GTG
Cuts at: 0 235 864
Size: 235 629
DrdI GACnn_nn'nnGTC
Cuts at: 0 355 864
Size: 355 509
EaeI y'GGCC_r
Cuts at: 0 237 864
Size: 237 627
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 478 864
Size: 478 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 541 695 864
Size: 449 92 154 169
Fragments arranged by size:
449 169 154 92
EcoO109I rG'GnC_Cy
Cuts at: 0 225 864
Size: 225 639
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 625 657 864
Size: 625 32 207
Fragments arranged by size:
625 207 32
HincII GTy'rAC
Cuts at: 0 816 864
Size: 816 48
HindIII A'AGCT_T
Cuts at: 0 4 864
Size: 4 860
MscI TGG'CCA
Cuts at: 0 239 864
Size: 239 625
MspA1I CmG'CkG
Cuts at: 0 262 835 864
Size: 262 573 29
Fragments arranged by size:
573 262 29
NsiI A_TGCA'T
Cuts at: 0 30 104 752 864
Size: 30 74 648 112
Fragments arranged by size:
648 112 74 30
NspI r_CATG'y
Cuts at: 0 102 754 864
Size: 102 652 110
Fragments arranged by size:
652 110 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 235 732 864
Size: 235 497 132
Fragments arranged by size:
497 235 132
PfoI T'CCnGG_A
Cuts at: 0 359 446 864
Size: 359 87 418
Fragments arranged by size:
418 359 87
PpuMI rG'GwC_Cy
Cuts at: 0 225 864
Size: 225 639
PstI C_TGCA'G
Cuts at: 0 261 864
Size: 261 603
PvuII CAG'CTG
Cuts at: 0 262 835 864
Size: 262 573 29
Fragments arranged by size:
573 262 29
SfcI C'TryA_G
Cuts at: 0 257 864
Size: 257 607
SmlI C'TyrA_G
Cuts at: 0 642 864
Size: 642 222
SphI G_CATG'C
Cuts at: 0 754 864
Size: 754 110
Tth111I GACn'n_nGTC
Cuts at: 0 690 864
Size: 690 174
Enzymes that do cut:
AcuI AlwNI ApoI BbsI BfrBI BglI Bme1580I BmrI
BpmI BpuEI BseRI BseYI BsgI BsmI Bsp1286I BtgI
DraIII DrdI EaeI EciI Eco57MI EcoO109I Hin4I HincII
HindIII MscI MspA1I NsiI NspI PflMI PfoI PpuMI
PstI PvuII SfcI SmlI SphI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI EagI EarI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
XbaI XcmI XhoI XmaI XmnI ZraI