Restriction map for pF1KB6592
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BbeI        BbsI        BbvCI       BciVI       
BclI        BcnI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstEII      BstPI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeIII      MfeI        MluI        MluNI       MroI        
MscI        MspI        MunI        NaeI        NarI        
NciI        NdeI        NgoMIV      NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmeI        PpuMI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Tsp45I      Tth111I     
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII         185  CGCG                 CG^CG
AflIII        576  ACGTGT               A^C[GA][CT]GT
BamHI         785  GGATCC               G^GATCC
BanI          761  GGTGCC               G^G[CT][GA]CC
BbrPI         575  CACGTG               CAC^GTG
BcgI          720  GCAGCGTTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1204  GCCCAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          60  AGATCT               A^GATCT
BsaAI         575  CACGTG               [CT]AC^GT[GA]
BsaBI         472  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         298  GAGGAG               GAGGAG(10/8)
BsgI          611  CTGCAC               GTGCAG(16/14)
BspHI          35  TCATGA               T^CATGA
BspMI         303  GCAGGT               ACCTGC(4/8)
BsrBI          45  GAGCGG               CCGCTC(-3/-3)
BsrDI         151  GCAATG               GCAATG(2/0)
BstBI         961  TTCGAA               TT^CGAA
BstUI         185  CGCG                 CG^CG
Cfr13I         27  GGCCC                G^G[ACGT]CC
DsaI         1196  CCATGG               C^C[GA][CT]GG
MamI          472  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        878  CAGCTG               C[AC]G^C[GT]G
MvnI          185  CGCG                 CG^CG
NcoI         1196  CCATGG               C^CATGG
NheI          735  GCTAGC               G^CTAGC
NspI          681  ACATGC               [GA]CATG^[CT]
NspV          961  TTCGAA               TT^CGAA
PflMI         372  CCAAGTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          284  GAGTC                GAGTC(4/5)
PmaCI         575  CACGTG               CAC^GTG
PmlI          575  CACGTG               CAC^GTG
PshAI         642  GACAATAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          992  CTGCAG               CTGCA^G
PvuII         878  CAGCTG               CAG^CTG
RcaI           35  TCATGA               T^CATGA
Sau96I         27  GGCCC                G^G[ACGT]CC
SfuI          961  TTCGAA               TT^CGAA
SspI          952  AATATT               AAT^ATT
StuI          671  AGGCCT               AGG^CCT
TfiI          529  GAATC                G^A[AT]TC
TspRI         691  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        372  CCAAGTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          804  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AlwNI          82  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              190  CAGTTGCTG           
AspHI         628  GAGCTC               G[AT]GC[AT]^C
              974  GAGCTC              
BanII         628  GAGCTC               G[GA]GC[CT]^C
              974  GAGCTC              
BmyI          628  GAGCTC               G[AGT]GC[ACT]^C
              974  GAGCTC              
BsaJI         263  CCTTGG               C^C[ACGT][ACGT]GG
             1196  CCATGG              
BsiHKAI       628  GAGCTC               G[AT]GC[AT]^C
              974  GAGCTC              
BsiYI         372  CCAAGTGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              486  CCAGGCAGAGG         
BslI          372  CCAAGTGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              486  CCAGGCAGAGG         
BsmAI         436  GAGAC                GTCTC(1/5)
              173  GTCTC               
Bsp1286I      628  GAGCTC               G[AGT]GC[ACT]^C
              974  GAGCTC              
BsrI          432  ACTGG                ACTGG(1/-1)
              481  CCAGT               
BssKI          87  CCTGG                ^CC[ACGT]GG
              486  CCAGG               
BssSI        1088  CACGAG               CACGAG(-5/-1)
              175  CTCGTG              
BstNI          87  CCTGG                CC^[AT]GG
              486  CCAGG               
BstXI         890  CCAGCATCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1059  CCAGATTGATGG        
EcoRI          39  GAATTC               G^AATTC
              386  GAATTC              
EcoRII         87  CCTGG                ^CC[AT]GG
              486  CCAGG               
EcoT14I       263  CCTTGG               C^C[AT][AT]GG
             1196  CCATGG              
HaeIII         27  GGCC                 GG^CC
              672  GGCC                
HindIII       128  AAGCTT               A^AGCTT
              137  AAGCTT              
HinfI         284  GAGTC                G^A[ACGT]TC
              529  GAATC               
HphI          260  TCACC                GGTGA(8/7)
              861  TCACC               
MseI          676  TTAA                 T^TAA
              749  TTAA                
MvaI           87  CCTGG                CC^[AT]GG
              486  CCAGG               
SacI          628  GAGCTC               GAGCT^C
              974  GAGCTC              
ScrFI          87  CCTGG                CC^[ACGT]GG
              486  CCAGG               
SfcI          875  CTACAG               C^T[GA][CT]AG
              992  CTGCAG              
StyI          263  CCTTGG               C^C[AT][AT]GG
             1196  CCATGG              
Tru9I         676  TTAA                 T^TAA
              749  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BfaI        BpmI        BstF5I      
BstYI       Cac8I       DdeI        DpnII       Fnu4HI      
FokI        ItaI        MaeI        MaeII       MboI        
MboII       MflI        MnlI        MslI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
SfaNI       TaqI        TseI        Tsp509I     TthHB8I     
XhoII       
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