Restriction map for pF1KB6592
 (Linear) (Six-base) MAPSORT of: KIBB6592.seq  Check: 4452  from: 1  to: 1218



KIBB6592.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     505    1218 
   Size:        505     713
 
AflIII A'CryG_T

Cuts at:      0     576    1218 
   Size:        576     642
 
AleI CACnn'nnGTG

Cuts at:      0     656    1218 
   Size:        656     562
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     791    1218 
   Size:        791     427
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     195    1218 
   Size:         87     108    1023

  Fragments arranged by size: 

                1023     108      87
 
ApoI r'AATT_y

Cuts at:      0      39     386     598     838    1218 
   Size:         39     347     212     240     380

  Fragments arranged by size: 

                 380     347     240     212      39
 
BamHI G'GATC_C

Cuts at:      0     785    1218 
   Size:        785     433
 
BanI G'GyrC_C

Cuts at:      0     761    1218 
   Size:        761     457
 
BanII G_rGCy'C

Cuts at:      0     632     978    1218 
   Size:        632     346     240

  Fragments arranged by size: 

                 632     346     240
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     709     743    1218 
   Size:        709      34     475

  Fragments arranged by size: 

                 709     475      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1210    1218 
   Size:       1210       8
 
BglII A'GATC_T

Cuts at:      0      60    1218 
   Size:         60    1158
 
BmtI G_CTAG'C

Cuts at:      0     739    1218 
   Size:        739     479
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      68    1218 
   Size:         68    1150
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     454     487    1218 
   Size:        131     323      33     731

  Fragments arranged by size: 

                 731     323     131      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     998    1218 
   Size:        998     220
 
BsaAI yAC'GTr

Cuts at:      0     577    1218 
   Size:        577     641
 
BsaBI GATnn'nnATC

Cuts at:      0     476    1218 
   Size:        476     742
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     313    1218 
   Size:        313     905
 
BseYI C'CCAG_C

Cuts at:      0      29    1218 
   Size:         29    1189
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     596    1218 
   Size:        596     622
 
BsiHKAI G_wGCw'C

Cuts at:      0     632     978    1218 
   Size:        632     346     240

  Fragments arranged by size: 

                 632     346     240
 
Bsp1286I G_dGCh'C

Cuts at:      0     632     978    1218 
   Size:        632     346     240

  Fragments arranged by size: 

                 632     346     240
 
BspHI T'CATG_A

Cuts at:      0      35    1218 
   Size:         35    1183
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     294    1218 
   Size:        294     924
 
BsrBI CCG'CTC

Cuts at:      0      47    1218 
   Size:         47    1171
 
BsrDI GCAATG_nn'

Cuts at:      0     158    1218 
   Size:        158    1060
 
BssSI C'ACGA_G

Cuts at:      0     175    1088    1218 
   Size:        175     913     130

  Fragments arranged by size: 

                 913     175     130
 
BstBI TT'CG_AA

Cuts at:      0     962    1218 
   Size:        962     256
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     897    1066    1218 
   Size:        897     169     152

  Fragments arranged by size: 

                 897     169     152
 
BstYI r'GATC_y

Cuts at:      0      60      83     272     785     986    1118    1218 
   Size:         60      23     189     513     201     132     100

  Fragments arranged by size: 

                 513     201     189     132     100      60      23
 
BtgI C'CryG_G

Cuts at:      0    1196    1218 
   Size:       1196      22
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1018    1218 
   Size:       1018     200
 
EcoICRI GAG'CTC

Cuts at:      0     630     976    1218 
   Size:        630     346     242

  Fragments arranged by size: 

                 630     346     242
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     454     487     505    1218 
   Size:        131     323      33      18     713

  Fragments arranged by size: 

                 713     323     131      33      18
 
EcoRI G'AATT_C

Cuts at:      0      39     386    1218 
   Size:         39     347     832

  Fragments arranged by size: 

                 832     347      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1002    1034    1218 
   Size:       1002      32     184

  Fragments arranged by size: 

                1002     184      32
 
HindIII A'AGCT_T

Cuts at:      0     128     137    1218 
   Size:        128       9    1081

  Fragments arranged by size: 

                1081     128       9
 
MslI CAynn'nnrTG

Cuts at:      0     656     853    1179    1218 
   Size:        656     197     326      39

  Fragments arranged by size: 

                 656     326     197      39
 
MspA1I CmG'CkG

Cuts at:      0     880    1218 
   Size:        880     338
 
NcoI C'CATG_G

Cuts at:      0    1196    1218 
   Size:       1196      22
 
NheI G'CTAG_C

Cuts at:      0     735    1218 
   Size:        735     483
 
NspI r_CATG'y

Cuts at:      0     685    1218 
   Size:        685     533
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     378    1218 
   Size:        378     840
 
PfoI T'CCnGG_A

Cuts at:      0      86    1218 
   Size:         86    1132
 
PmlI CAC'GTG

Cuts at:      0     577    1218 
   Size:        577     641
 
PshAI GACnn'nnGTC

Cuts at:      0     646    1218 
   Size:        646     572
 
PsiI TTA'TAA

Cuts at:      0     428     560    1218 
   Size:        428     132     658

  Fragments arranged by size: 

                 658     428     132
 
PstI C_TGCA'G

Cuts at:      0     996    1218 
   Size:        996     222
 
PvuII CAG'CTG

Cuts at:      0     880    1218 
   Size:        880     338
 
SacI G_AGCT'C

Cuts at:      0     632     978    1218 
   Size:        632     346     240

  Fragments arranged by size: 

                 632     346     240
 
SfcI C'TryA_G

Cuts at:      0     875     992    1218 
   Size:        875     117     226

  Fragments arranged by size: 

                 875     226     117
 
SspI AAT'ATT

Cuts at:      0     954    1218 
   Size:        954     264
 
StuI AGG'CCT

Cuts at:      0     673    1218 
   Size:        673     545
 
StyI C'CwwG_G

Cuts at:      0     263    1196    1218 
   Size:        263     933      22

  Fragments arranged by size: 

                 933     263      22
 
TatI w'GTAC_w

Cuts at:      0     413    1218 
   Size:        413     805
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     201     233     745     777    1118    1150    1218 
   Size:        201      32     512      32     341      32      68

  Fragments arranged by size: 

                 512     341     201      68      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0     804    1218 
   Size:        804     414
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI    BamHI     BanI
    BanII     BcgI     BglI    BglII     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaBI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BssSI    BstBI    BstXI    BstYI     BtgI
     EciI  EcoICRI  Eco57MI    EcoRI    Hin4I  HindIII     MslI   MspA1I
     NcoI     NheI     NspI    PflMI     PfoI     PmlI    PshAI     PsiI
     PstI    PvuII     SacI     SfcI     SspI     StuI     StyI     TatI
     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BlpI Bme1580I    BmgBI     BmrI    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrFI    BsrGI
   BssHII   BstAPI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PpiI    PpuMI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SwaI    TaqII  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI
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