Restriction map for pF1KB6961
##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       Bsp1286I    Bsp1407I    BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstNI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      CpoI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRI       EcoRII      EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HinfI       
HpaII       KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        TfiI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AciI           76  GCGG                 CCGC(-3/-1)
AcsI          283  AAATTT               [GA]^AATT[CT]
AluI           17  AGCT                 AG^CT
ApoI          283  AAATTT               [GA]^AATT[CT]
AvaII          21  GGACC                G^G[AT]CC
BanI          106  GGCACC               G^G[CT][GA]CC
BbsI          210  GAAGAC               GAAGAC(2/6)
BbvI          169  GCAGC                GCAGC(8/12)
BpuAI         210  GAAGAC               GAAGAC(2/6)
BsaJI          98  CCATGG               C^C[ACGT][ACGT]GG
BsiYI          32  CCGAATGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           32  CCGAATGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          80  GAGAC                GTCTC(1/5)
BsmFI         475  GGGAC                GGGAC(10/14)
BsmI           34  GAATGC               GAATGC(1/-1)
BspDI         112  ATCGAT               AT^CGAT
BsrI          198  ACTGG                ACTGG(1/-1)
BstEII         42  GGTAACC              G^GT[ACGT]ACC
BstF5I        486  GGATG                GGATG(2/0)
BstPI          42  GGTAACC              G^GT[ACGT]ACC
Cfr13I         21  GGACC                G^G[ACGT]CC
ClaI          112  ATCGAT               AT^CGAT
DdeI          294  CTCAG                C^T[ACGT]AG
DraI           58  TTTAAA               TTT^AAA
DsaI           98  CCATGG               C^C[GA][CT]GG
EcoO65I        42  GGTAACC              G^GT[ACGT]ACC
EcoT14I        98  CCATGG               C^C[AT][AT]GG
FauI           76  GCGGG                CCCGC(4/6)
Fnu4HI        169  GCAGC                GC^[ACGT]GC
FokI          486  GGATG                GGATG(9/13)
HaeIII         96  GGCC                 GG^CC
HpaI            6  GTTAAC               GTT^AAC
HphI          186  TCACC                GGTGA(8/7)
ItaI          169  GCAGC                GC^[ACGT]GC
MaeIII         43  GTAAC                ^GT[ACGT]AC
MspA1I         16  CAGCTG               C[AC]G^C[GT]G
MwoI          172  GCAAACAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI           98  CCATGG               C^CATGG
NlaIII         99  CATG                 CATG^
NlaIV         106  GGCACC               GG[ACGT]^[ACGT]CC
PvuII          16  CAGCTG               CAG^CTG
Sau96I         21  GGACC                G^G[ACGT]CC
SfaNI         487  GATGC                GCATC(5/9)
SfcI          134  CTACAG               C^T[GA][CT]AG
StyI           98  CCATGG               C^C[AT][AT]GG
TaqI          113  TCGA                 T^CGA
TseI          169  GCAGC                G^C[AT]GC
TthHB8I       113  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AlwI          148  GATCC                GGATC(4/5)
              118  GGATC               
DpnII         119  GATC                 ^GATC
              148  GATC                
EarI          453  CTCTTC               CTCTTC(1/4)
              331  GAAGAG              
HincII          6  GTTAAC               GT[CT]^[GA]AC
              507  GTCAAC              
HindII          6  GTTAAC               GT[CT]^[GA]AC
              507  GTCAAC              
Ksp632I       453  CTCTTC               CTCTTC(1/4)
              331  GAAGAG              
MboI          119  GATC                 ^GATC
              148  GATC                
MseI            7  TTAA                 T^TAA
               59  TTAA                
NdeII         119  GATC                 ^GATC
              148  GATC                
Sau3AI        119  GATC                 ^GATC
              148  GATC                
SmlI           84  CTTGAG               C^T[CT][GA]AG
              371  CTCAAG              
SspI          262  AATATT               AAT^ATT
              421  AATATT              
Tru9I           7  TTAA                 T^TAA
               59  TTAA                
TspRI         122  CACTG                CA[GC]TG[ACGT][ACGT]^
              137  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

MboII       MnlI        Tsp509I     
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