Restriction map for pF1KB9792
 (Linear) (Six-base) MAPSORT of: KIBB9792.seq  Check: 7010  from: 1  to: 630



KIBB9792.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      96     630 
   Size:         96     534
 
AfeI AGC'GCT

Cuts at:      0     238     358     630 
   Size:        238     120     272

  Fragments arranged by size: 

                 272     238     120
 
AflIII A'CryG_T

Cuts at:      0     275     630 
   Size:        275     355
 
AlwNI CAG_nnn'CTG

Cuts at:      0      42     147     630 
   Size:         42     105     483

  Fragments arranged by size: 

                 483     105      42
 
AvaI C'yCGr_G

Cuts at:      0     309     630 
   Size:        309     321
 
BanI G'GyrC_C

Cuts at:      0     304     630 
   Size:        304     326
 
BanII G_rGCy'C

Cuts at:      0     521     630 
   Size:        521     109
 
BbeI G_GCGC'C

Cuts at:      0     308     630 
   Size:        308     322
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     583     630 
   Size:        583      47
 
Bme1580I G_kGCm'C

Cuts at:      0     316     487     630 
   Size:        316     171     143

  Fragments arranged by size: 

                 316     171     143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616     630 
   Size:        616      14
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     450     630 
   Size:        450     180
 
BsaAI yAC'GTr

Cuts at:      0     120     425     630 
   Size:        120     305     205

  Fragments arranged by size: 

                 305     205     120
 
BsaBI GATnn'nnATC

Cuts at:      0      61     630 
   Size:         61     569
 
BsaHI Gr'CG_yC

Cuts at:      0     305     385     630 
   Size:        305      80     245

  Fragments arranged by size: 

                 305     245      80
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     430     460     630 
   Size:        430      30     170

  Fragments arranged by size: 

                 430     170      30
 
BseYI C'CCAG_C

Cuts at:      0      42     147     630 
   Size:         42     105     483

  Fragments arranged by size: 

                 483     105      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     630 
   Size:         34     596
 
Bsp1286I G_dGCh'C

Cuts at:      0     316     487     521     630 
   Size:        316     171      34     109

  Fragments arranged by size: 

                 316     171     109      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      96     630 
   Size:         96     534
 
BssHII G'CGCG_C

Cuts at:      0      18      20     630 
   Size:         18       2     610

  Fragments arranged by size: 

                 610      18       2
 
BssSI C'ACGA_G

Cuts at:      0     507     630 
   Size:        507     123
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      12     630 
   Size:         12     618
 
BstYI r'GATC_y

Cuts at:      0      56     630 
   Size:         56     574
 
BtgI C'CryG_G

Cuts at:      0     581     630 
   Size:        581      49
 
DraIII CAC_nnn'GTG

Cuts at:      0      12     630 
   Size:         12     618
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     388     630 
   Size:        388     242
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616     630 
   Size:        616      14
 
HaeII r_GCGC'y

Cuts at:      0     240     308     360     630 
   Size:        240      68      52     270

  Fragments arranged by size: 

                 270     240      68      52
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     262     292     294     324     630 
   Size:        262      30       2      30     306

  Fragments arranged by size: 

                 306     262      30      30       2
 
KasI G'GCGC_C

Cuts at:      0     304     630 
   Size:        304     326
 
MluI A'CGCG_T

Cuts at:      0     275     630 
   Size:        275     355
 
MspA1I CmG'CkG

Cuts at:      0     160     170     630 
   Size:        160      10     460

  Fragments arranged by size: 

                 460     160      10
 
NarI GG'CG_CC

Cuts at:      0     305     630 
   Size:        305     325
 
NcoI C'CATG_G

Cuts at:      0     581     630 
   Size:        581      49
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      42      78     494     630 
   Size:         42      36     416     136

  Fragments arranged by size: 

                 416     136      42      36
 
PmlI CAC'GTG

Cuts at:      0     120     630 
   Size:        120     510
 
SexAI A'CCwGG_T

Cuts at:      0     440     630 
   Size:        440     190
 
SfoI GGC'GCC

Cuts at:      0     306     630 
   Size:        306     324
 
StyI C'CwwG_G

Cuts at:      0     320     362     368     533     581     630 
   Size:        320      42       6     165      48      49

  Fragments arranged by size: 

                 320     165      49      48      42       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     333     630 
   Size:        333     297
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     448     630 
   Size:        448     182
 

 Enzymes that do cut:

     AarI     AfeI   AflIII    AlwNI     AvaI     BanI    BanII     BbeI
     BbsI Bme1580I     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI     BsgI Bsp1286I    BspMI   BssHII    BssSI   BstAPI    BstYI
     BtgI   DraIII     EciI  Eco57MI    HaeII    Hin4I     KasI     MluI
   MspA1I     NarI     NcoI    PflMI     PmlI    SexAI     SfoI     StyI
    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MmeI     MscI     MslI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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