Restriction map for pF1KB5035
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HaeIII      HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvaI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AciI           68  GCGG                 CCGC(-3/-1)
AcsI          285  GAATTT               [GA]^AATT[CT]
AfaI          476  GTAC                 GT^AC
AlwI          376  GGATC                GGATC(4/5)
AocI          340  CCTCAGG              CC^T[ACGT]AGG
ApoI          285  GAATTT               [GA]^AATT[CT]
AspHI         131  GAGCTC               G[AT]GC[AT]^C
AvaI          280  CTCGAG               C^[CT]CG[GA]G
BfaI          178  CTAG                 C^TAG
BglII          97  AGATCT               A^GATCT
BpmI          543  CTCCAG               CTGGAG(16/14)
BseRI         415  GAGGAG               GAGGAG(10/8)
BsgI          189  CTGCAC               GTGCAG(16/14)
BsiHKAI       131  GAGCTC               G[AT]GC[AT]^C
BsmI           21  GCATTC               GAATGC(1/-1)
BsoBI         280  CTCGAG               C^[CT]CG[GA]G
BstF5I        429  GGATG                GGATG(2/0)
BstYI          97  AGATCT               [GA]^GATC[CT]
Bsu36I        340  CCTCAGG              CC^T[ACGT]AGG
CfoI          141  GCGC                 GCG^C
DdeI          341  CTCAG                C^T[ACGT]AG
DraI          309  TTTAAA               TTT^AAA
DraII         336  GGGTCCT              [GA]G^G[ACGT]CC[CT]
Eco81I        340  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      336  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       453  CCATGG               C^C[AT][AT]GG
FauI           68  GCGGG                CCCGC(4/6)
FokI          429  GGATG                GGATG(9/13)
HhaI          141  GCGC                 GCG^C
HinP1I        141  GCGC                 G^CGC
MaeI          178  CTAG                 C^TAG
MflI           97  AGATCT               [GA]^GATC[CT]
NcoI          453  CCATGG               C^CATGG
NlaIII        454  CATG                 CATG^
NlaIV         336  GGGTCC               GG[ACGT]^[ACGT]CC
PaeR7I        280  CTCGAG               C^TCGAG
PpuMI         336  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         328  CAGCTG               CAG^CTG
RsaI          476  GTAC                 GT^AC
SacI          131  GAGCTC               GAGCT^C
SfaNI         485  GCATC                GCATC(5/9)
SmlI          280  CTCGAG               C^T[CT][GA]AG
StyI          453  CCATGG               C^C[AT][AT]GG
Tsp509I       286  AATT                 ^AATT
XhoI          280  CTCGAG               C^TCGAG
XhoII          97  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AvaII         181  GGACC                G^G[AT]CC
              337  GGTCC               
BanII           6  GAGCCC               G[GA]GC[CT]^C
              131  GAGCTC              
BcnI           48  CCGGG                CC^[GC]GG
              194  CCCGG               
BsaJI          34  CCACGG               C^C[ACGT][ACGT]GG
              453  CCATGG              
BsiYI          32  CCCCACGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              517  CCGAGTATTGG         
BslI           32  CCCCACGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              517  CCGAGTATTGG         
BssKI          48  CCGGG                ^CC[ACGT]GG
              194  CCCGG               
Cac8I         269  GCCAGC               GC[ACGT]^[ACGT]GC
              512  GCCTGC              
Cfr13I        181  GGACC                G^G[ACGT]CC
              337  GGTCC               
DsaI           34  CCACGG               C^C[GA][CT]GG
              453  CCATGG              
HapII          48  CCGG                 C^CGG
              195  CCGG                
HpaII          48  CCGG                 C^CGG
              195  CCGG                
MboII         250  GAAGA                GAAGA(8/7)
              388  TCTTC               
MseI          160  TTAA                 T^TAA
              310  TTAA                
MspA1I         66  CAGCGG               C[AC]G^C[GT]G
              328  CAGCTG              
MspI           48  CCGG                 C^CGG
              195  CCGG                
NciI           48  CCGGG                CC^[GC]GG
              194  CCCGG               
PstI           16  CTGCAG               CTGCA^G
              331  CTGCAG              
Sau96I        181  GGACC                G^G[ACGT]CC
              337  GGTCC               
ScrFI          48  CCGGG                CC^[ACGT]GG
              194  CCCGG               
SfcI           16  CTGCAG               C^T[GA][CT]AG
              331  CTGCAG              
TaqI          126  TCGA                 T^CGA
              281  TCGA                
Tru9I         160  TTAA                 T^TAA
              310  TTAA                
Tsp45I        491  GTGAC                ^GT[GC]AC
              530  GTGAC               
TthHB8I       126  TCGA                 T^CGA
              281  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AluI        BbvI        BmyI        Bsp1286I    BsrI        
DpnII       Fnu4HI      HinfI       ItaI        MaeIII      
MboI        MnlI        MwoI        NdeII       Sau3AI      
TfiI        TseI        TspRI       
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