Restriction map for pF1KB3876
##################################
# NO CUT ENZYMES (  185 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspHI       AvaII       AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MroI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I        206  GGTACC               G^GTACC
AciI          461  GCGG                 CCGC(-3/-1)
AcsI          445  GAATTC               [GA]^AATT[CT]
AfaI          207  GTAC                 GT^AC
AlwNI         296  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          445  GAATTC               [GA]^AATT[CT]
Asp718I       206  GGTACC               G^GTACC
AspI          476  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          161  CCCGAG               C^[CT]CG[GA]G
BalI           65  TGGCCA               TGG^CCA
BciVI         224  GTATCC               GTATCC(6/5)
BcnI           96  CCCGG                CC^[GC]GG
BmyI          118  GTGCCC               G[AGT]GC[ACT]^C
BsaAI         169  TACGTG               [CT]AC^GT[GA]
BsaWI          20  ACCGGA               [AT]^CCGG[AT]
BsmFI         159  GTCCC                GGGAC(10/14)
BsoBI         161  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      118  GTGCCC               G[AGT]GC[ACT]^C
BspHI         134  TCATGA               T^CATGA
BspMI         339  ACCTGC               ACCTGC(4/8)
DraIII        398  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           65  TGGCCA               [CT]^GGCC[GA]
EcoNI          42  CCTTTTTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         445  GAATTC               G^AATTC
HphI          331  GGTGA                GGTGA(8/7)
KpnI          206  GGTACC               GGTAC^C
MboII          15  GAAGA                GAAGA(8/7)
MluNI          65  TGGCCA               TGG^CCA
MscI           65  TGGCCA               TGG^CCA
MseI           47  TTAA                 T^TAA
NciI           96  CCCGG                CC^[GC]GG
NspI           83  ACATGC               [GA]CATG^[CT]
PflFI         476  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         295  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          134  TCATGA               T^CATGA
RsaI          207  GTAC                 GT^AC
SexAI         399  ACCTGGT              A^CC[AT]GGT
SfaNI           6  GATGC                GCATC(5/9)
SfcI          378  CTACAG               C^T[GA][CT]AG
SmlI          154  CTCAAG               C^T[CT][GA]AG
StuI          307  AGGCCT               AGG^CCT
Tru9I          47  TTAA                 T^TAA
Tsp509I       446  AATT                 ^AATT
Tth111I       476  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        295  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AluI          101  AGCT                 AG^CT
              363  AGCT                
BanI          206  GGTACC               G^G[CT][GA]CC
              403  GGTGCC              
BpmI          189  CTGGAG               CTGGAG(16/14)
              325  CTGGAG              
BstF5I        276  CATCC                GGATG(2/0)
               75  GGATG               
BstNI         188  CCTGG                CC^[AT]GG
              400  CCTGG               
EcoRII        188  CCTGG                ^CC[AT]GG
              400  CCTGG               
FokI          276  CATCC                GGATG(9/13)
               75  GGATG               
HapII          21  CCGG                 C^CGG
               97  CCGG                
HinfI          56  GAGTC                G^A[ACGT]TC
              347  GACTC               
HpaII          21  CCGG                 C^CGG
               97  CCGG                
MaeII         170  ACGT                 A^CGT
              477  ACGT                
MspI           21  CCGG                 C^CGG
               97  CCGG                
MvaI          188  CCTGG                CC^[AT]GG
              400  CCTGG               
MwoI           93  GCACCCGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              430  GCTGGGTCAGC         
NlaIV         206  GGTACC               GG[ACGT]^[ACGT]CC
              403  GGTGCC              
PleI          347  GACTC                GAGTC(4/5)
               56  GAGTC               


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

BsaJI       BsiYI       BslI        BsmAI       BsrI        
BssKI       Cac8I       DdeI        HaeIII      MnlI        
NlaIII      ScrFI       
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