Restriction map for pF1KB3876
 (Circular) (Six-base) MAPSORT of: pF1KB3876.seq  Check: 568  from: 1  to: 3608



pF1KB3876  3608 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:    646     646 
   Size:       3608
 
Acc65I G'GTAC_C

Cuts at:    314     624     314 
   Size:        310    3298
 
AclI AA'CG_TT

Cuts at:   3448    3448 
   Size:       3608
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1433    1865    2612    2880    1433 
   Size:        432     747     268    2161

  Fragments arranged by size: 

                2161     747     432     268
 
AflIII A'CryG_T

Cuts at:    960    2064    3041     960 
   Size:       1104     977    1527

  Fragments arranged by size: 

                1527    1104     977
 
AgeI A'CCGG_T

Cuts at:   1996    1996 
   Size:       3608
 
AlwNI CAG_nnn'CTG

Cuts at:    409     913    2480     409 
   Size:        504    1567    1537

  Fragments arranged by size: 

                1567    1537     504
 
ApaLI G'TGCA_C

Cuts at:   2378    2378 
   Size:       3608
 
ApoI r'AATT_y

Cuts at:    553     612    2961    3217     553 
   Size:         59    2349     256     944

  Fragments arranged by size: 

                2349     944     256      59
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3608
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3608
 
AvaI C'yCGr_G

Cuts at:    269     628     269 
   Size:        359    3249
 
BamHI G'GATC_C

Cuts at:    633     633 
   Size:       3608
 
BanI G'GyrC_C

Cuts at:    314     511     624    1038    1286    1321     314 
   Size:        197     113     414     248      35    2601

  Fragments arranged by size: 

                2601     414     248     197     113      35
 
BanII G_rGCy'C

Cuts at:    622    1652     622 
   Size:       1030    2578
 
BbeI G_GCGC'C

Cuts at:   1042    1290    1042 
   Size:        248    3360
 
BciVI GTATCCnnnnn_n'

Cuts at:    343    1506    2267    3608     343 
   Size:       1163     761    1341     343

  Fragments arranged by size: 

                1341    1163     761     343
 
BclI T'GATC_A

Cuts at:   1128    1128 
   Size:       3608
 
BglII A'GATC_T

Cuts at:   1123    3160    1123 
   Size:       2037    1571
 
BlpI GC'TnA_GC

Cuts at:    720     720 
   Size:       3608
 
Bme1580I G_kGCm'C

Cuts at:    230    1233    1326    2382     230 
   Size:       1003      93    1056    1456

  Fragments arranged by size: 

                1456    1056    1003      93
 
BmgBI CAC'GTC

Cuts at:    586     586 
   Size:       3608
 
BmrI ACTGGGnnnn_n'

Cuts at:    820     901    1236    3380     820 
   Size:         81     335    2144    1048

  Fragments arranged by size: 

                2144    1048     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1975    1975 
   Size:       3608
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    318     454     318 
   Size:        136    3472
 
Bpu10I CC'TnA_GC

Cuts at:    197     197 
   Size:       3608
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    247     784    1663    2155    2453     247 
   Size:        537     879     492     298    1402

  Fragments arranged by size: 

                1402     879     537     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3312    3312 
   Size:       3608
 
BsaAI yAC'GTr

Cuts at:    279     961     279 
   Size:        682    2926
 
BsaBI GATnn'nnATC

Cuts at:      6    1122    3159       6 
   Size:       1116    2037     455

  Fragments arranged by size: 

                2037    1116     455
 
BsaHI Gr'CG_yC

Cuts at:   1039    1287    1989    3485    1039 
   Size:        248     702    1496    1162

  Fragments arranged by size: 

                1496    1162     702     248
 
BsaWI w'CCGG_w

Cuts at:    128     797    1318    1996    2270    2417    2828     128 
   Size:        669     521     678     274     147     411     908

  Fragments arranged by size: 

                 908     678     669     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    155     185     155 
   Size:         30    3578
 
BseYI C'CCAG_C

Cuts at:    532     538    1033    1791    2368    2864     532 
   Size:          6     495     758     577     496    1276

  Fragments arranged by size: 

                1276     758     577     496     495       6
 
BsiEI CG_ry'CG

Cuts at:    104    1150    1196    2404     104 
   Size:       1046      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1046      46
 
BsiHKAI G_wGCw'C

Cuts at:    622    1400    1590    2382     622 
   Size:        778     190     792    1848

  Fragments arranged by size: 

                1848     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    230     622    1233    1326    1400    1590    1652    2382     230 
   Size:        392     611      93      74     190      62     730    1456

  Fragments arranged by size: 

                1456     730     611     392     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2828    2828 
   Size:       3608
 
BspHI T'CATG_A

Cuts at:    242    2784     242 
   Size:       2542    1066
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    456     658     872    1174    1555     456 
   Size:        202     214     302     381    2509

  Fragments arranged by size: 

                2509     381     302     214     202
 
BsrBI CCG'CTC

Cuts at:     50    1900    1954    3438    3603      50 
   Size:       1850      54    1484     165      55

  Fragments arranged by size: 

                1850    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1520    1520 
   Size:       3608
 
BsrFI r'CCGG_y

Cuts at:   1606    1787    1996    1606 
   Size:        181     209    3218

  Fragments arranged by size: 

                3218     209     181
 
BssSI C'ACGA_G

Cuts at:   1879    2237    1879 
   Size:        358    3250
 
BstAPI GCAn_nnn'nTGC

Cuts at:    985     985 
   Size:       3608
 
BstBI TT'CG_AA

Cuts at:   1969    1969 
   Size:       3608
 
BstYI r'GATC_y

Cuts at:      1     633    1100    1123    1458    1704    2716    2802    2814 
   Size:        632     467      23     335     246    1012      86      12

Cuts at:   2814    3160       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     632     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    599     599 
   Size:       3608
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1558    1739    1902    1558 
   Size:        181     163    3264

  Fragments arranged by size: 

                3264     181     163
 
BtsI GCAGTG_nn'

Cuts at:    851     851 
   Size:       3608
 
DraI TTT'AAA

Cuts at:    607     607 
   Size:       3608
 
DraIII CAC_nnn'GTG

Cuts at:    511     511 
   Size:       3608
 
DrdI GACnn_nn'nnGTC

Cuts at:   1314    2172    1314 
   Size:        858    2750
 
EaeI y'GGCC_r

Cuts at:    173    1193    1367    1758    1785    3130     173 
   Size:       1020     174     391      27    1345     651

  Fragments arranged by size: 

                1345    1020     651     391     174      27
 
EagI C'GGCC_G

Cuts at:   1193    1193 
   Size:       3608
 
EarI CTCTTCn'nnn_

Cuts at:   1631    1841    1631 
   Size:        210    3398
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    584    1816    2126    2272    3418     584 
   Size:       1232     310     146    1146     774

  Fragments arranged by size: 

                1232    1146     774     310     146
 
EcoICRI GAG'CTC

Cuts at:    620     620 
   Size:       3608
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    318     454    1433    1865    2612    2880     318 
   Size:        136     979     432     747     268    1046

  Fragments arranged by size: 

                1046     979     747     432     268     136
 
EcoNI CCTnn'n_nnAGG

Cuts at:    154     154 
   Size:       3608
 
EcoO109I rG'GnC_Cy

Cuts at:    747     747 
   Size:       3608
 
EcoRI G'AATT_C

Cuts at:    553     612     553 
   Size:         59    3549
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1627    1627 
   Size:       3608
 
FspI TGC'GCA

Cuts at:   1389    1389 
   Size:       3608
 
HaeII r_GCGC'y

Cuts at:   1042    1290    2312    3291    1042 
   Size:        248    1022     979    1359

  Fragments arranged by size: 

                1359    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1450    1480    1482    1512    1450 
   Size:         30       2      30    3546

  Fragments arranged by size: 

                3546      30      30       2
 
HincII GTy'rAC

Cuts at:    647     647 
   Size:       3608
 
KasI G'GCGC_C

Cuts at:   1038    1286    1038 
   Size:        248    3360
 
KpnI G_GTAC'C

Cuts at:    318     628     318 
   Size:        310    3298
 
MluI A'CGCG_T

Cuts at:   3041    3041 
   Size:       3608
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2279    2463    2279 
   Size:        184    3424
 
MscI TGG'CCA

Cuts at:    175    1369     175 
   Size:       1194    2414
 
MslI CAynn'nnrTG

Cuts at:    112    1597    1724     112 
   Size:       1485     127    1996

  Fragments arranged by size: 

                1996    1485     127
 
MspA1I CmG'CkG

Cuts at:    719    1033    1393    2406    2651     719 
   Size:        314     360    1013     245    1676

  Fragments arranged by size: 

                1676    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1789    1789 
   Size:       3608
 
NarI GG'CG_CC

Cuts at:   1039    1287    1039 
   Size:        248    3360
 
NgoMIV G'CCGG_C

Cuts at:   1787    1787 
   Size:       3608
 
NspI r_CATG'y

Cuts at:    195     661    1161    2068     195 
   Size:        466     500     907    1735

  Fragments arranged by size: 

                1735     907     500     466
 
PciI A'CATG_T

Cuts at:   2064    2064 
   Size:       3608
 
PflMI CCAn_nnn'nTGG

Cuts at:    409     409 
   Size:       3608
 
PmeI GTTT'AAAC

Cuts at:    607     607 
   Size:       3608
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2747    2779    2747 
   Size:         32    3576
 
PstI C_TGCA'G

Cuts at:    655    1340     655 
   Size:        685    2923
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3608
 
PvuII CAG'CTG

Cuts at:   1033    1393    1033 
   Size:        360    3248
 
RsrII CG'GwC_CG

Cuts at:   1803    1803 
   Size:       3608
 
SacI G_AGCT'C

Cuts at:    622     622 
   Size:       3608
 
SalI G'TCGA_C

Cuts at:    645     645 
   Size:       3608
 
SapI GCTCTTCn'nnn_

Cuts at:   1631    1841    1631 
   Size:        210    3398
 
SbfI CC_TGCA'GG

Cuts at:    655     655 
   Size:       3608
 
ScaI AGT'ACT

Cuts at:   3125    3125 
   Size:       3608
 
SexAI A'CCwGG_T

Cuts at:    507     507 
   Size:       3608
 
SfcI C'TryA_G

Cuts at:     33     486     651    1336    2329    2520      33 
   Size:        453     165     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     453     191     165
 
SfoI GGC'GCC

Cuts at:   1040    1288    1040 
   Size:        248    3360
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3608
 
SmaI CCC'GGG

Cuts at:    630     630 
   Size:       3608
 
SmlI C'TyrA_G

Cuts at:    262     763    1678    2170    2432     262 
   Size:        501     915     492     262    1438

  Fragments arranged by size: 

                1438     915     501     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3608
 
SphI G_CATG'C

Cuts at:    661    1161     661 
   Size:        500    3108
 
StuI AGG'CCT

Cuts at:    417     417 
   Size:       3608
 
StyI C'CwwG_G

Cuts at:    742    1096     742 
   Size:        354    3254
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1191    1324    1783    1992    1191 
   Size:        133     459     209    2807

  Fragments arranged by size: 

                2807     459     209     133
 
TatI w'GTAC_w

Cuts at:   3123    3123 
   Size:       3608
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    155     187    3125    3157     155 
   Size:         32    2938      32     606

  Fragments arranged by size: 

                2938     606      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    587    1405     587 
   Size:        818    2790
 
XbaI T'CTAG_A

Cuts at:    639     639 
   Size:       3608
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1103    1103 
   Size:       3608
 
XmaI C'CCGG_G

Cuts at:    628     628 
   Size:       3608
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI    PflMI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI     BmtI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I     BtgI     ClaI    CspCI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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