Restriction map for pF1KB3461
##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tsp45I      
Tth111I     XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          595  GTAGAC               GT^[AC][GT]AC
AspHI         275  GAGCAC               G[AT]GC[AT]^C
BciVI         222  GGATAC               GTATCC(6/5)
BmyI          275  GAGCAC               G[AGT]GC[ACT]^C
BsiHKAI       275  GAGCAC               G[AT]GC[AT]^C
BsiYI          34  CCAAATACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           34  CCAAATACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         481  GAGAC                GTCTC(1/5)
Bsp1286I      275  GAGCAC               G[AGT]GC[ACT]^C
BspHI         135  TCATGA               T^CATGA
BstAPI        571  GCAAATTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        485  CATCC                GGATG(2/0)
Cac8I         360  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI           14  GCGC                 GCG^C
Cfr13I         31  GGGCC                G^G[ACGT]CC
EarI          587  GAAGAG               CTCTTC(1/4)
EcoRV         301  GATATC               GAT^ATC
FokI          485  CATCC                GGATG(9/13)
HhaI           14  GCGC                 GCG^C
HinP1I         14  GCGC                 G^CGC
Ksp632I       587  GAAGAG               CTCTTC(1/4)
MaeIII        641  GTAAC                ^GT[ACGT]AC
MboII         587  GAAGA                GAAGA(8/7)
MseI          344  TTAA                 T^TAA
PflMI          34  CCAAATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         227  CAGCTG               CAG^CTG
RcaI          135  TCATGA               T^CATGA
Sau96I         31  GGGCC                G^G[ACGT]CC
ScaI           69  AGTACT               AGT^ACT
SpeI           66  ACTAGT               A^CTAGT
SspI          516  AATATT               AAT^ATT
TaqI            9  TCGA                 T^CGA
TfiI          627  GAATC                G^A[AT]TC
Tru9I         344  TTAA                 T^TAA
TspRI         631  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I         9  TCGA                 T^CGA
Van91I         34  CCAAATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AciI           86  CCGC                 CCGC(-3/-1)
              488  CCGC                
AcsI          139  GAATTT               [GA]^AATT[CT]
              573  AAATTC              
ApoI          139  GAATTT               [GA]^AATT[CT]
              573  AAATTC              
BbvI          283  GCAGC                GCAGC(8/12)
              163  GCTGC               
BsrI           40  ACTGG                ACTGG(1/-1)
               57  CCAGT               
Fnu4HI        163  GCTGC                GC^[ACGT]GC
              283  GCAGC               
ItaI          163  GCTGC                GC^[ACGT]GC
              283  GCAGC               
MspA1I         86  CCGCTG               C[AC]G^C[GT]G
              227  CAGCTG              
PleI           82  GAGTC                GAGTC(4/5)
              316  GAGTC               
SfcI          200  CTACAG               C^T[GA][CT]AG
              267  CTACAG              
TseI          163  GCTGC                G^C[AT]GC
              283  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AfaI        AluI        BfaI        BseRI       BssKI       
BstNI       EcoRII      HaeIII      HinfI       MaeI        
MnlI        MvaI        MwoI        NlaIII      RsaI        
ScrFI       Tsp509I     
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