Restriction map for pF1KB3105
##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaJI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraI        
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        KasI        KpnI        KspI        
MaeII       MaeIII      MfeI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
MvaI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NspI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tsp45I      TspRI       Tth111I     Van91I      
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AcsI          154  GAATTT               [GA]^AATT[CT]
ApoI          154  GAATTT               [GA]^AATT[CT]
BcnI          136  CCCGG                CC^[GC]GG
BglII         340  AGATCT               A^GATCT
BsaBI         304  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          458  GGTCTC               GGTCTC(1/5)
BseRI         318  GAGGAG               GAGGAG(10/8)
BspMI         377  GCAGGT               ACCTGC(4/8)
BssKI         136  CCCGG                ^CC[ACGT]GG
BstAPI        175  GCAGCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII         379  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        209  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          401  GAAGAG               CTCTTC(1/4)
EcoNI         449  CCTTCTCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      379  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I         1  ATGCAT               ATGCA^T
HaeIII        271  GGCC                 GG^CC
HindIII       357  AAGCTT               A^AGCTT
HphI          247  GGTGA                GGTGA(8/7)
Ksp632I       401  GAAGAG               CTCTTC(1/4)
MamI          304  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        176  CAGCTG               C[AC]G^C[GT]G
NciI          136  CCCGG                CC^[GC]GG
NlaIV         380  GGTCCC               GG[ACGT]^[ACGT]CC
NsiI            1  ATGCAT               ATGCA^T
PaeR7I        299  CTCGAG               C^TCGAG
PleI          321  GAGTC                GAGTC(4/5)
PpuMI         379  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         176  CAGCTG               CAG^CTG
ScrFI         136  CCCGG                CC^[ACGT]GG
SmlI          299  CTCGAG               C^T[CT][GA]AG
TfiI           10  GATTC                G^A[AT]TC
XbaI          388  TCTAGA               T^CTAGA
XcmI          274  CCACCTCCCTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          299  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AlwI          163  GATCC                GGATC(4/5)
              446  GATCC               
AvaII         100  GGACC                G^G[AT]CC
              380  GGTCC               
BsiYI         280  CCCTCTTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              449  CCTTCTCTAGG         
BslI          280  CCCTCTTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              449  CCTTCTCTAGG         
BsmAI         417  GTCTC                GTCTC(1/5)
              459  GTCTC               
BsmFI         381  GTCCC                GGGAC(10/14)
              441  GTCCC               
BstYI         162  AGATCC               [GA]^GATC[CT]
              340  AGATCT              
HapII         137  CCGG                 C^CGG
              372  CCGG                
HinfI          10  GATTC                G^A[ACGT]TC
              321  GAGTC               
HpaII         137  CCGG                 C^CGG
              372  CCGG                
MflI          162  AGATCC               [GA]^GATC[CT]
              340  AGATCT              
MspI          137  CCGG                 C^CGG
              372  CCGG                
Tsp509I        74  AATT                 ^AATT
              155  AATT                
XhoII         162  AGATCC               [GA]^GATC[CT]
              340  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AluI        AvaI        BbvI        BfaI        BsoBI       
Cfr13I      DpnII       Fnu4HI      ItaI        MaeI        
MboI        MboII       MnlI        MwoI        NdeII       
Sau3AI      Sau96I      SfaNI       TaqI        TseI        
TthHB8I     
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