Restriction map for pF1KB9771
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcsI        AcyI        AflII       AgeI        AhdI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BseAI       BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        BspDI       BspEI       BspHI       BsrBI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       CfoI        ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MluI        MluNI       MroI        MscI        
MspA1I      MvnI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AciI         1564  GCGG                 CCGC(-3/-1)
AclI          460  AACGTT               AA^CGTT
Alw44I        188  GTGCAC               G^TGCAC
AocI         1216  CCTGAGG              CC^T[ACGT]AGG
ApaLI         188  GTGCAC               G^TGCAC
AspHI         188  GTGCAC               G[AT]GC[AT]^C
AvaI            7  CTCGAG               C^[CT]CG[GA]G
BbsI         1225  GAAGAC               GAAGAC(2/6)
BbvCI        1319  CCTCAGC              CCTCAGC(-5/-2)
BcgI          909  GCATCCTGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1295  CTAG                 C^TAG
BpuAI        1225  GAAGAC               GAAGAC(2/6)
BsaWI        1257  ACCGGA               [AT]^CCGG[AT]
BsgI         1558  GTGCAG               GTGCAG(16/14)
BsiHKAI       188  GTGCAC               G[AT]GC[AT]^C
BsoBI           7  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      827  TGTACA               T^GTACA
BspMI         802  GCAGGT               ACCTGC(4/8)
BsrGI         827  TGTACA               T^GTACA
BstNI         855  CCTGG                CC^[AT]GG
BstXI         941  CCAGTGGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         549  GGATCT               [GA]^GATC[CT]
Bsu36I       1216  CCTGAGG              CC^T[ACGT]AGG
DraI         1495  TTTAAA               TTT^AAA
EaeI          135  TGGCCG               [CT]^GGCC[GA]
Eco81I       1216  CCTGAGG              CC^T[ACGT]AGG
EcoRII        855  CCTGG                ^CC[AT]GG
FseI         1453  GGCCGGCC             GGCCGG^CC
HincII        402  GTTGAC               GT[CT]^[GA]AC
HindII        402  GTTGAC               GT[CT]^[GA]AC
MaeI         1295  CTAG                 C^TAG
MaeII         461  ACGT                 A^CGT
MfeI          608  CAATTG               C^AATTG
MflI          549  GGATCT               [GA]^GATC[CT]
MslI         1131  CACCAGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          608  CAATTG               C^AATTG
MvaI          855  CCTGG                CC^[AT]GG
NaeI         1454  GCCGGC               GCC^GGC
NgoMIV       1454  GCCGGC               G^CCGGC
PacI          640  TTAATTAA             TTAAT^TAA
PaeR7I          7  CTCGAG               C^TCGAG
Psp1406I      460  AACGTT               AA^CGTT
PstI         1106  CTGCAG               CTGCA^G
SexAI         854  ACCTGGT              A^CC[AT]GGT
SmlI            7  CTCGAG               C^T[CT][GA]AG
SphI          819  GCATGC               GCATG^C
SspBI         827  TGTACA               T^GTACA
XhoI            7  CTCGAG               C^TCGAG
XhoII         549  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          828  GTAC                 GT^AC
             1384  GTAC                
AflIII        423  ACATGT               A^C[GA][CT]GT
              694  ACATGT              
AlwNI         803  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1094  CAGGGGCTG           
BcnI          620  CCCGG                CC^[GC]GG
              812  CCCGG               
BsaJI         757  CCATGG               C^C[ACGT][ACGT]GG
              870  CCATGG              
BspLU11I      423  ACATGT               A^CATGT
              694  ACATGT              
BsrDI         171  CATTGC               GCAATG(2/0)
             1209  CATTGC              
BsrFI         435  GCCGGT               [GA]^CCGG[CT]
             1454  GCCGGC              
BstAPI        726  GCAGAAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              802  GCAGGTTCTGC         
Cfr10I        435  GCCGGT               [GA]^CCGG[CT]
             1454  GCCGGC              
DraII         751  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1330  GGGTCCC             
DsaI          757  CCATGG               C^C[GA][CT]GG
              870  CCATGG              
EcoNI          92  CCTTTAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1531  CCTCTGGAAGG         
EcoO109I      751  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1330  GGGTCCC             
EcoT14I       757  CCATGG               C^C[AT][AT]GG
              870  CCATGG              
NciI          620  CCCGG                CC^[GC]GG
              812  CCCGG               
NcoI          757  CCATGG               C^CATGG
              870  CCATGG              
PleI          448  GACTC                GAGTC(4/5)
               19  GAGTC               
PpuMI         751  AGGACCC              [GA]G^G[AT]CC[CT]
             1330  GGGTCCC             
RsaI          828  GTAC                 GT^AC
             1384  GTAC                
SapI          840  GAAGAGC              GCTCTTC(1/4)
             1171  GAAGAGC             
SfcI         1106  CTGCAG               C^T[GA][CT]AG
             1158  CTGTAG              
StyI          757  CCATGG               C^C[AT][AT]GG
              870  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AluI        AlwI        AvaII       BbvI        BmyI        
BpmI        BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BssSI       BstF5I      
Cac8I       Cfr13I      DdeI        DpnII       EarI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com