Restriction map for pF1KB9771
 (Linear) (Six-base) MAPSORT of: KIBB9771.seq  Check: 7082  from: 1  to: 1614



KIBB9771.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AclI AA'CG_TT

Cuts at:      0     461    1614 
   Size:        461    1153
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     549    1614 
   Size:        549    1065
 
AflIII A'CryG_T

Cuts at:      0     423     694    1614 
   Size:        423     271     920

  Fragments arranged by size: 

                 920     423     271
 
AleI CACnn'nnGTG

Cuts at:      0    1135    1614 
   Size:       1135     479
 
AlwNI CAG_nnn'CTG

Cuts at:      0     808    1099    1614 
   Size:        808     291     515

  Fragments arranged by size: 

                 808     515     291
 
ApaLI G'TGCA_C

Cuts at:      0     188    1614 
   Size:        188    1426
 
AvaI C'yCGr_G

Cuts at:      0       7    1614 
   Size:          7    1607
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1232    1614 
   Size:       1232     382
 
BbvCI CC'TCA_GC

Cuts at:      0    1320    1614 
   Size:       1320     294
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     898     932    1614 
   Size:        898      34     682

  Fragments arranged by size: 

                 898     682      34
 
Bme1580I G_kGCm'C

Cuts at:      0     109     192     966    1614 
   Size:        109      83     774     648

  Fragments arranged by size: 

                 774     648     109      83
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1420    1614 
   Size:       1420     194
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     904     907    1614 
   Size:        904       3     707

  Fragments arranged by size: 

                 904     707       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     240    1005    1614 
   Size:         37     203     765     609

  Fragments arranged by size: 

                 765     609     203      37
 
Bpu10I CC'TnA_GC

Cuts at:      0    1085    1320    1614 
   Size:       1085     235     294

  Fragments arranged by size: 

                1085     294     235
 
BsaWI w'CCGG_w

Cuts at:      0    1257    1614 
   Size:       1257     357
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     433     463    1614 
   Size:        433      30    1151

  Fragments arranged by size: 

                1151     433      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     880     896    1607    1614 
   Size:        880      16     711       7

  Fragments arranged by size: 

                 880     711      16       7
 
BseYI C'CCAG_C

Cuts at:      0    1334    1614 
   Size:       1334     280
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1579    1614 
   Size:       1579      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     192    1614 
   Size:        192    1422
 
Bsp1286I G_dGCh'C

Cuts at:      0     109     192     966    1614 
   Size:        109      83     774     648

  Fragments arranged by size: 

                 774     648     109      83
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     793    1614 
   Size:        793     821
 
BsrDI GCAATG_nn'

Cuts at:      0     170    1208    1614 
   Size:        170    1038     406

  Fragments arranged by size: 

                1038     406     170
 
BsrFI r'CCGG_y

Cuts at:      0     435    1454    1614 
   Size:        435    1019     160

  Fragments arranged by size: 

                1019     435     160
 
BsrGI T'GTAC_A

Cuts at:      0     827    1614 
   Size:        827     787
 
BssSI C'ACGA_G

Cuts at:      0     241     350     370    1614 
   Size:        241     109      20    1244

  Fragments arranged by size: 

                1244     241     109      20
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     732     808    1614 
   Size:        732      76     806

  Fragments arranged by size: 

                 806     732      76
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     948    1614 
   Size:        948     666
 
BstYI r'GATC_y

Cuts at:      0     549    1614 
   Size:        549    1065
 
Bsu36I CC'TnA_GG

Cuts at:      0    1217    1614 
   Size:       1217     397
 
BtgI C'CryG_G

Cuts at:      0     757     870    1614 
   Size:        757     113     744

  Fragments arranged by size: 

                 757     744     113
 
BtsI GCAGTG_nn'

Cuts at:      0     109    1170    1614 
   Size:        109    1061     444

  Fragments arranged by size: 

                1061     444     109
 
DraI TTT'AAA

Cuts at:      0    1497    1614 
   Size:       1497     117
 
EaeI y'GGCC_r

Cuts at:      0     135    1614 
   Size:        135    1479
 
EarI CTCTTCn'nnn_

Cuts at:      0     696     835    1166    1303    1614 
   Size:        696     139     331     137     311

  Fragments arranged by size: 

                 696     331     311     139     137
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1579    1614 
   Size:       1579      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     240     549    1005    1614 
   Size:         37     203     309     456     609

  Fragments arranged by size: 

                 609     456     309     203      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      96    1535    1614 
   Size:         96    1439      79

  Fragments arranged by size: 

                1439      96      79
 
EcoO109I rG'GnC_Cy

Cuts at:      0     752    1331    1614 
   Size:        752     579     283

  Fragments arranged by size: 

                 752     579     283
 
FseI GG_CCGG'CC

Cuts at:      0    1458    1614 
   Size:       1458     156
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     433     465    1417    1449    1583    1614 
   Size:        433      32     952      32     134      31

  Fragments arranged by size: 

                 952     433     134      32      32      31
 
HincII GTy'rAC

Cuts at:      0     404    1614 
   Size:        404    1210
 
MfeI C'AATT_G

Cuts at:      0     608    1614 
   Size:        608    1006
 
MslI CAynn'nnrTG

Cuts at:      0    1135    1614 
   Size:       1135     479
 
NaeI GCC'GGC

Cuts at:      0    1456    1614 
   Size:       1456     158
 
NcoI C'CATG_G

Cuts at:      0     757     870    1614 
   Size:        757     113     744

  Fragments arranged by size: 

                 757     744     113
 
NgoMIV G'CCGG_C

Cuts at:      0    1454    1614 
   Size:       1454     160
 
NspI r_CATG'y

Cuts at:      0     427     698     823    1614 
   Size:        427     271     125     791

  Fragments arranged by size: 

                 791     427     271     125
 
PacI TTA_AT'TAA

Cuts at:      0     644    1614 
   Size:        644     970
 
PciI A'CATG_T

Cuts at:      0     423     694    1614 
   Size:        423     271     920

  Fragments arranged by size: 

                 920     423     271
 
PpuMI rG'GwC_Cy

Cuts at:      0     752    1331    1614 
   Size:        752     579     283

  Fragments arranged by size: 

                 752     579     283
 
PspXI vC'TCGA_Gb

Cuts at:      0       7    1614 
   Size:          7    1607
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     573     605    1614 
   Size:        573      32    1009

  Fragments arranged by size: 

                1009     573      32
 
PstI C_TGCA'G

Cuts at:      0    1110    1614 
   Size:       1110     504
 
SanDI GG'GwC_CC

Cuts at:      0    1331    1614 
   Size:       1331     283
 
SapI GCTCTTCn'nnn_

Cuts at:      0     835    1166    1614 
   Size:        835     331     448

  Fragments arranged by size: 

                 835     448     331
 
SexAI A'CCwGG_T

Cuts at:      0     854    1614 
   Size:        854     760
 
SfcI C'TryA_G

Cuts at:      0    1106    1158    1614 
   Size:       1106      52     456

  Fragments arranged by size: 

                1106     456      52
 
SmlI C'TyrA_G

Cuts at:      0       7    1614 
   Size:          7    1607
 
SphI G_CATG'C

Cuts at:      0     823    1614 
   Size:        823     791
 
StyI C'CwwG_G

Cuts at:      0     757     870    1614 
   Size:        757     113     744

  Fragments arranged by size: 

                 757     744     113
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     339    1402    1614 
   Size:        339    1063     212

  Fragments arranged by size: 

                1063     339     212
 
TatI w'GTAC_w

Cuts at:      0     827    1614 
   Size:        827     787
 
XhoI C'TCGA_G

Cuts at:      0       7    1614 
   Size:          7    1607
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AleI    AlwNI    ApaLI     AvaI     BbsI
    BbvCI     BcgI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     DraI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FseI    Hin4I   HincII     MfeI     MslI     NaeI     NcoI   NgoMIV
     NspI     PacI     PciI    PpuMI    PspXI     PsrI     PstI    SanDI
     SapI    SexAI     SfcI     SmlI     SphI     StyI    TaqII     TatI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI   BssHII    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     MscI   MspA1I     NarI     NdeI     NheI     NotI     NruI     NsiI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI     ZraI
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