Restriction map for pF1KB6206
 (Linear) (Six-base) MAPSORT of: KIBB6206.seq  Check: 2785  from: 1  to: 2145



KIBB6206.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AclI AA'CG_TT

Cuts at:      0     698    2145 
   Size:        698    1447
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     819     960    1317    1401    1653    1737    2145 
   Size:        819     141     357      84     252      84     408

  Fragments arranged by size: 

                 819     408     357     252     141      84      84
 
AleI CACnn'nnGTG

Cuts at:      0    1964    2145 
   Size:       1964     181
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      57    2145 
   Size:         57    2088
 
ApaLI G'TGCA_C

Cuts at:      0    1395    2145 
   Size:       1395     750
 
ApoI r'AATT_y

Cuts at:      0     276     588    1283    1367    1619    1703    1871    2039 
   Size:        276     312     695      84     252      84     168     168

Cuts at:   2039    2145 
   Size:        106

  Fragments arranged by size: 

                 695     312     276     252     168     168     106      84
                  84
 
AseI AT'TA_AT

Cuts at:      0     602    2145 
   Size:        602    1543
 
AvaI C'yCGr_G

Cuts at:      0     239    2145 
   Size:        239    1906
 
BanII G_rGCy'C

Cuts at:      0    1933    2017    2145 
   Size:       1933      84     128

  Fragments arranged by size: 

                1933     128      84
 
BfrBI ATG'CAT

Cuts at:      0    1947    2145 
   Size:       1947     198
 
Bme1580I G_kGCm'C

Cuts at:      0    1399    2145 
   Size:       1399     746
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1889    2145 
   Size:       1889     256
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     369    2145 
   Size:        369    1776
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     217     983    1145    1229    1313    1397    1481 
   Size:        151      66     766     162      84      84      84      84

Cuts at:   1481    1565    1649    1733    1817    2069    2145 
   Size:         84      84      84      84     252      76

  Fragments arranged by size: 

                 766     252     162     151      84      84      84      84
                  84      84      84      84      76      66
 
Bpu10I CC'TnA_GC

Cuts at:      0     554    2145 
   Size:        554    1591
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     438     702    2145 
   Size:        280     158     264    1443

  Fragments arranged by size: 

                1443     280     264     158
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     127     961    2145 
   Size:        127     834    1184

  Fragments arranged by size: 

                1184     834     127
 
BsaAI yAC'GTr

Cuts at:      0     318    2145 
   Size:        318    1827
 
BsaWI w'CCGG_w

Cuts at:      0     339    2145 
   Size:        339    1806
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     256     286    1876    1906    2145 
   Size:        256      30    1590      30     239

  Fragments arranged by size: 

                1590     256     239      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91     277     358    2145 
   Size:         91     186      81    1787

  Fragments arranged by size: 

                1787     186      91      81
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237     927    2145 
   Size:        237     690    1218

  Fragments arranged by size: 

                1218     690     237
 
BsiHKAI G_wGCw'C

Cuts at:      0    1399    1933    2017    2145 
   Size:       1399     534      84     128

  Fragments arranged by size: 

                1399     534     128      84
 
BsiWI C'GTAC_G

Cuts at:      0    1724    2145 
   Size:       1724     421
 
BsmI GAATG_Cn'

Cuts at:      0      64    1324    2145 
   Size:         64    1260     821

  Fragments arranged by size: 

                1260     821      64
 
Bsp1286I G_dGCh'C

Cuts at:      0    1399    1933    2017    2145 
   Size:       1399     534      84     128

  Fragments arranged by size: 

                1399     534     128      84
 
BspHI T'CATG_A

Cuts at:      0     606    2145 
   Size:        606    1539
 
BsrGI T'GTAC_A

Cuts at:      0     326     939    1356    1524    2145 
   Size:        326     613     417     168     621

  Fragments arranged by size: 

                 621     613     417     326     168
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1907    2145 
   Size:       1907     238
 
BstBI TT'CG_AA

Cuts at:      0    1139    2145 
   Size:       1139    1006
 
BstYI r'GATC_y

Cuts at:      0    1258    2145 
   Size:       1258     887
 
BtgI C'CryG_G

Cuts at:      0    1007    2145 
   Size:       1007    1138
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     338     373    1554    1589    2145 
   Size:        338      35    1181      35     556

  Fragments arranged by size: 

                1181     556     338      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     318     846    2145 
   Size:        318     528    1299

  Fragments arranged by size: 

                1299     528     318
 
EarI CTCTTCn'nnn_

Cuts at:      0     200     838    2145 
   Size:        200     638    1307

  Fragments arranged by size: 

                1307     638     200
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2136    2145 
   Size:       2136       9
 
EcoICRI GAG'CTC

Cuts at:      0    1931    2015    2145 
   Size:       1931      84     130

  Fragments arranged by size: 

                1931     130      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     217     819     960     983    1145    1229    1313 
   Size:        151      66     602     141      23     162      84      84

Cuts at:   1313    1317    1397    1401    1481    1565    1649    1653    1733 
   Size:          4      80       4      80      84      84       4      80

Cuts at:   1733    1737    1817    2069    2145 
   Size:          4      80     252      76

  Fragments arranged by size: 

                 602     252     162     151     141      84      84      84
                  84      80      80      80      80      76      66      23
                   4       4       4       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1923    2145 
   Size:       1923     222
 
EcoO109I rG'GnC_Cy

Cuts at:      0     210     234    2145 
   Size:        210      24    1911

  Fragments arranged by size: 

                1911     210      24
 
EcoRI G'AATT_C

Cuts at:      0     276    1283    1367    1619    1703    2039    2145 
   Size:        276    1007      84     252      84     336     106

  Fragments arranged by size: 

                1007     336     276     252     106      84      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     274    2145 
   Size:        274    1871
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204    1042    1074    2145 
   Size:        172      32     838      32    1071

  Fragments arranged by size: 

                1071     838     172      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      39     650    1062    1964    2145 
   Size:         39     611     412     902     181

  Fragments arranged by size: 

                 902     611     412     181      39
 
MspA1I CmG'CkG

Cuts at:      0      90     195    1091    2145 
   Size:         90     105     896    1054

  Fragments arranged by size: 

                1054     896     105      90
 
NcoI C'CATG_G

Cuts at:      0    1007    2145 
   Size:       1007    1138
 
NsiI A_TGCA'T

Cuts at:      0    1949    2145 
   Size:       1949     196
 
PasI CC'CwG_GG

Cuts at:      0     156    2145 
   Size:        156    1989
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     318    2145 
   Size:        318    1827
 
PmlI CAC'GTG

Cuts at:      0     318    2145 
   Size:        318    1827
 
PpuMI rG'GwC_Cy

Cuts at:      0     210    2145 
   Size:        210    1935
 
PvuII CAG'CTG

Cuts at:      0      90     195    1091    2145 
   Size:         90     105     896    1054

  Fragments arranged by size: 

                1054     896     105      90
 
SacI G_AGCT'C

Cuts at:      0    1933    2017    2145 
   Size:       1933      84     128

  Fragments arranged by size: 

                1933     128      84
 
SfcI C'TryA_G

Cuts at:      0    1046    2145 
   Size:       1046    1099
 
SmlI C'TyrA_G

Cuts at:      0     259     417     681    2145 
   Size:        259     158     264    1464

  Fragments arranged by size: 

                1464     264     259     158
 
StyI C'CwwG_G

Cuts at:      0    1007    2145 
   Size:       1007    1138
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1267    2145 
   Size:       1267     878
 
TatI w'GTAC_w

Cuts at:      0     326     541     939    1144    1356    1480    1524    2145 
   Size:        326     215     398     205     212     124      44     621

  Fragments arranged by size: 

                 621     398     326     215     212     205     124      44
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     621     653    2145 
   Size:        621      32    1492

  Fragments arranged by size: 

                1492     621      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1183    2145 
   Size:       1183     962
 
XmnI GAAnn'nnTTC

Cuts at:      0     789    1246    1498    1582    1666    2043    2086    2145 
   Size:        789     457     252      84      84     377      43      59

  Fragments arranged by size: 

                 789     457     377     252      84      84      59      43
            
 

 Enzymes that do cut:

     AclI     AcuI     AleI     AlfI    ApaLI     ApoI     AseI     AvaI
    BanII    BfrBI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspHI    BsrGI   BstAPI    BstBI    BstYI     BtgI
    CspCI   DraIII     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    Hin4I     MslI   MspA1I     NcoI     NsiI     PasI
    PflMI     PmlI    PpuMI    PvuII     SacI     SfcI     SmlI     StyI
    TaqII     TatI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI     ApaI     AscI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII     BlpI    BmgBI     BmtI    BsaBI    BsaHI    BseYI
    BsiEI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
     DraI     DrdI     EaeI     EagI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PciI     PfoI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     XbaI     XcmI     XhoI     XmaI     ZraI
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