Restriction map for pF1KB5306
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaLI       AscI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcnI        
BfrI        BglI        BglII       BlnI        BsaAI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspEI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       Cfr10I      
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HapII       HpaI        HpaII       
KasI        KpnI        KspI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AciI          161  CCGC                 CCGC(-3/-1)
AcyI          782  GACGCC               G[GA]^CG[CT]C
AhdI         1600  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1910  CCTCAGG              CC^T[ACGT]AGG
ApaI         1915  GGGCCC               GGGCC^C
AseI          517  ATTAAT               AT^TAAT
AsnI          517  ATTAAT               AT^TAAT
AspEI        1600  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1403  CCCGAG               C^[CT]CG[GA]G
BcgI           78  CGAGATAATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1025  GTATCC               GTATCC(6/5)
BclI           93  TGATCA               T^GATCA
BfaI          976  CTAG                 C^TAG
BlpI          808  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      808  GCTAAGC              GC^T[ACGT]AGC
BsaHI         782  GACGCC               G[GA]^CG[CT]C
BsoBI        1403  CCCGAG               C^[CT]CG[GA]G
BspDI         850  ATCGAT               AT^CGAT
BsrDI         178  GCAATG               GCAATG(2/0)
Bst1107I     1451  GTATAC               GTA^TAC
BstZ17I      1451  GTATAC               GTA^TAC
Bsu36I       1910  CCTCAGG              CC^T[ACGT]AGG
CelII         808  GCTAAGC              GC^T[ACGT]AGC
CfoI          738  GCGC                 GCG^C
ClaI          850  ATCGAT               AT^CGAT
DsaI          930  CCGTGG               C^C[GA][CT]GG
Eam1105I     1600  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1910  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1617  GAATTC               G^AATTC
FbaI           93  TGATCA               T^GATCA
HhaI          738  GCGC                 GCG^C
Hin1I         782  GACGCC               G[GA]^CG[CT]C
HinP1I        738  GCGC                 G^CGC
HincII        712  GTCAAC               GT[CT]^[GA]AC
HindII        712  GTCAAC               GT[CT]^[GA]AC
MaeI          976  CTAG                 C^TAG
MslI          679  CACTTGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI         1847  GCATGC               [GA]CATG^[CT]
PpuMI           9  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI         517  ATTAAT               AT^TAAT
PstI           14  CTGCAG               CTGCA^G
SacI         1886  GAGCTC               GAGCT^C
SmlI         1624  CTTGAG               C^T[CT][GA]AG
SphI         1847  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          660  GTCTAC               GT^[AC][GT]AC
             1451  GTATAC              
AcsI         1617  GAATTC               [GA]^AATT[CT]
             1771  GAATTT              
ApoI         1617  GAATTC               [GA]^AATT[CT]
             1771  GAATTT              
AspHI         583  GTGCTC               G[AT]GC[AT]^C
             1886  GAGCTC              
BanI           34  GGCACC               G^G[CT][GA]CC
              934  GGCACC              
BanII        1886  GAGCTC               G[GA]GC[CT]^C
             1915  GGGCCC              
BsaBI        1500  GATTACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1744  GATAAGAATC          
BsaI          328  GAGACC               GGTCTC(1/5)
              496  GGTCTC              
BsiHKAI       583  GTGCTC               G[AT]GC[AT]^C
             1886  GAGCTC              
BsmFI           9  GGGAC                GGGAC(10/14)
             1598  GGGAC               
BsmI          915  GAATGC               GAATGC(1/-1)
             1859  GAATGC              
BspHI         993  TCATGA               T^CATGA
             1226  TCATGA              
BspMI          12  ACCTGC               ACCTGC(4/8)
             1361  ACCTGC              
BstXI         419  CCAATGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              678  CCACTTGGGTGG        
DraII           9  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1914  AGGGCCC             
EarI          822  CTCTTC               CTCTTC(1/4)
             1927  GAAGAG              
EcoO109I        9  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1914  AGGGCCC             
HindIII       336  AAGCTT               A^AGCTT
             1042  AAGCTT              
Ksp632I       822  CTCTTC               CTCTTC(1/4)
             1927  GAAGAG              
MamI         1500  GATTACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1744  GATAAGAATC          
MspA1I        665  CAGCTG               C[AC]G^C[GT]G
             1118  CAGCTG              
PvuII         665  CAGCTG               CAG^CTG
             1118  CAGCTG              
RcaI          993  TCATGA               T^CATGA
             1226  TCATGA              
SfcI           14  CTGCAG               C^T[GA][CT]AG
              662  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AfaI        AluI        AvaII       BbsI        BbvI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HgaI        HinfI       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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