Restriction map for pF1KB5306
 (Linear) (Six-base) MAPSORT of: KIBB5306.seq  Check: 7268  from: 1  to: 1938



KIBB5306.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0     661    1452    1938 
   Size:        661     791     486

  Fragments arranged by size: 

                 791     661     486
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1601    1938 
   Size:       1601     337
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1605    1938 
   Size:       1605     333
 
AleI CACnn'nnGTG

Cuts at:      0     683    1938 
   Size:        683    1255
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1538    1570    1938 
   Size:       1538      32     368

  Fragments arranged by size: 

                1538     368      32
 
ApaI G_GGCC'C

Cuts at:      0    1919    1938 
   Size:       1919      19
 
ApoI r'AATT_y

Cuts at:      0    1617    1771    1938 
   Size:       1617     154     167

  Fragments arranged by size: 

                1617     167     154
 
AseI AT'TA_AT

Cuts at:      0     518    1938 
   Size:        518    1420
 
AvaI C'yCGr_G

Cuts at:      0    1403    1938 
   Size:       1403     535
 
BanI G'GyrC_C

Cuts at:      0      34     934    1938 
   Size:         34     900    1004

  Fragments arranged by size: 

                1004     900      34
 
BanII G_rGCy'C

Cuts at:      0    1890    1919    1938 
   Size:       1890      29      19

  Fragments arranged by size: 

                1890      29      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     350     415    1544    1938 
   Size:        350      65    1129     394

  Fragments arranged by size: 

                1129     394     350      65
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      67     101    1938 
   Size:         67      34    1837

  Fragments arranged by size: 

                1837      67      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1036    1938 
   Size:       1036     902
 
BclI T'GATC_A

Cuts at:      0      93    1938 
   Size:         93    1845
 
BlpI GC'TnA_GC

Cuts at:      0     809    1938 
   Size:        809    1129
 
Bme1580I G_kGCm'C

Cuts at:      0     900    1919    1938 
   Size:        900    1019      19

  Fragments arranged by size: 

                1019     900      19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     940    1938 
   Size:        940     998
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     689    1163    1816    1938 
   Size:        689     474     653     122

  Fragments arranged by size: 

                 689     653     474     122
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1645    1938 
   Size:       1645     293
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     322     502    1938 
   Size:        322     180    1436

  Fragments arranged by size: 

                1436     322     180
 
BsaBI GATnn'nnATC

Cuts at:      0    1504    1748    1938 
   Size:       1504     244     190

  Fragments arranged by size: 

                1504     244     190
 
BsaHI Gr'CG_yC

Cuts at:      0     783    1938 
   Size:        783    1155
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     364    1181    1880    1938 
   Size:        364     817     699      58

  Fragments arranged by size: 

                 817     699     364      58
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1136    1382    1859    1938 
   Size:       1136     246     477      79

  Fragments arranged by size: 

                1136     477     246      79
 
BsiHKAI G_wGCw'C

Cuts at:      0     587    1890    1938 
   Size:        587    1303      48

  Fragments arranged by size: 

                1303     587      48
 
BsmI GAATG_Cn'

Cuts at:      0     921    1865    1938 
   Size:        921     944      73

  Fragments arranged by size: 

                 944     921      73
 
Bsp1286I G_dGCh'C

Cuts at:      0     587     900    1890    1919    1938 
   Size:        587     313     990      29      19

  Fragments arranged by size: 

                 990     587     313      29      19
 
BspHI T'CATG_A

Cuts at:      0     993    1226    1938 
   Size:        993     233     712

  Fragments arranged by size: 

                 993     712     233
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      21    1370    1938 
   Size:         21    1349     568

  Fragments arranged by size: 

                1349     568      21
 
BsrDI GCAATG_nn'

Cuts at:      0     185    1938 
   Size:        185    1753
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     426     685    1938 
   Size:        426     259    1253

  Fragments arranged by size: 

                1253     426     259
 
BstZ17I GTA'TAC

Cuts at:      0    1453    1938 
   Size:       1453     485
 
Bsu36I CC'TnA_GG

Cuts at:      0    1911    1938 
   Size:       1911      27
 
BtgI C'CryG_G

Cuts at:      0     930    1938 
   Size:        930    1008
 
ClaI AT'CG_AT

Cuts at:      0     851    1938 
   Size:        851    1087
 
EarI CTCTTCn'nnn_

Cuts at:      0     828    1922    1938 
   Size:        828    1094      16

  Fragments arranged by size: 

                1094     828      16
 
EcoICRI GAG'CTC

Cuts at:      0    1888    1938 
   Size:       1888      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     689    1163    1601    1816    1938 
   Size:        689     474     438     215     122

  Fragments arranged by size: 

                 689     474     438     215     122
 
EcoO109I rG'GnC_Cy

Cuts at:      0      10    1915    1938 
   Size:         10    1905      23

  Fragments arranged by size: 

                1905      23      10
 
EcoRI G'AATT_C

Cuts at:      0    1617    1938 
   Size:       1617     321
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     418    1938 
   Size:        418    1520
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     340     372     643     675     840     872    1591    1623 
   Size:        340      32     271      32     165      32     719      32

Cuts at:   1623    1938 
   Size:        315

  Fragments arranged by size: 

                 719     340     315     271     165      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     714    1938 
   Size:        714    1224
 
HindIII A'AGCT_T

Cuts at:      0     336    1042    1938 
   Size:        336     706     896

  Fragments arranged by size: 

                 896     706     336
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     546    1938 
   Size:        546    1392
 
MslI CAynn'nnrTG

Cuts at:      0     683    1938 
   Size:        683    1255
 
MspA1I CmG'CkG

Cuts at:      0     667    1120    1938 
   Size:        667     453     818

  Fragments arranged by size: 

                 818     667     453
 
NspI r_CATG'y

Cuts at:      0    1851    1938 
   Size:       1851      87
 
PfoI T'CCnGG_A

Cuts at:      0    1556    1938 
   Size:       1556     382
 
PpuMI rG'GwC_Cy

Cuts at:      0      10    1938 
   Size:         10    1928
 
PspOMI G'GGCC_C

Cuts at:      0    1915    1938 
   Size:       1915      23
 
PstI C_TGCA'G

Cuts at:      0      18    1938 
   Size:         18    1920
 
PvuII CAG'CTG

Cuts at:      0     667    1120    1938 
   Size:        667     453     818

  Fragments arranged by size: 

                 818     667     453
 
SacI G_AGCT'C

Cuts at:      0    1890    1938 
   Size:       1890      48
 
SfcI C'TryA_G

Cuts at:      0      14     662    1938 
   Size:         14     648    1276

  Fragments arranged by size: 

                1276     648      14
 
SmlI C'TyrA_G

Cuts at:      0    1624    1938 
   Size:       1624     314
 
SphI G_CATG'C

Cuts at:      0    1851    1938 
   Size:       1851      87
 
StyI C'CwwG_G

Cuts at:      0       5     302     402    1200    1938 
   Size:          5     297     100     798     738

  Fragments arranged by size: 

                 798     738     297     100       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      43     673     691     847    1938 
   Size:         43     630      18     156    1091

  Fragments arranged by size: 

                1091     630     156      43      18
 
TatI w'GTAC_w

Cuts at:      0     504    1571    1938 
   Size:        504    1067     367

  Fragments arranged by size: 

                1067     504     367
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AleI     AloI     ApaI     ApoI     AseI
     AvaI     BanI    BanII     BbsI     BcgI    BciVI     BclI     BlpI
 Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI    Hin4I   HincII  HindIII     MmeI     MslI   MspA1I
     NspI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     SfcI
     SmlI     SphI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI    AlwNI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI
   Bpu10I    BsaAI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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