Restriction map for pF1KB5209
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AvaI        AviII       AvrII       
BbeI        BbvCI       BciVI       BfrI        BlnI        
BlpI        Bpu1102I    BsaI        BsaWI       BseAI       
BsiEI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRV       EcoT22I     FseI        FspI        
HaeII       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NarI        NcoI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          964  GAATTC               [GA]^AATT[CT]
AcyI          114  GGCGTC               G[GA]^CG[CT]C
AlwNI         847  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          541  CCTCAGG              CC^T[ACGT]AGG
ApoI          964  GAATTC               [GA]^AATT[CT]
BamHI        1419  GGATCC               G^GATCC
BbrPI        1204  CACGTG               CAC^GTG
BcgI         1544  GCAATGCTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1362  TGATCA               T^GATCA
BcnI           51  CCCGG                CC^[GC]GG
BglII         797  AGATCT               A^GATCT
BsaBI        1395  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         114  GGCGTC               G[GA]^CG[CT]C
BsiWI        1097  CGTACG               C^GTACG
BsrFI          46  GCCGGC               [GA]^CCGG[CT]
BssSI         922  CACGAG               CACGAG(-5/-1)
BstAPI        138  GCACTGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        981  GGTGACC              G^GT[ACGT]ACC
BstPI         981  GGTGACC              G^GT[ACGT]ACC
Bsu36I        541  CCTCAGG              CC^T[ACGT]AGG
Cfr10I         46  GCCGGC               [GA]^CCGG[CT]
Eco81I        541  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       981  GGTGACC              G^GT[ACGT]ACC
EcoRI         964  GAATTC               G^AATTC
FbaI         1362  TGATCA               T^GATCA
Hin1I         114  GGCGTC               G[GA]^CG[CT]C
MamI         1395  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          603  CATTCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI           46  GCCGGC               GCC^GGC
NciI           51  CCCGG                CC^[GC]GG
NgoMIV         46  GCCGGC               G^CCGGC
PmaCI        1204  CACGTG               CAC^GTG
PmlI         1204  CACGTG               CAC^GTG
PpuMI         926  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         849  GACTCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI         1315  CTTGAG               C^T[CT][GA]AG
SphI         1157  GCATGC               GCATG^C
StuI          659  AGGCCT               AGG^CCT
TfiI          444  GAATC                G^A[AT]TC
XcmI          842  CCATGCAGACTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   42 enzymes) #
###################################

AccII          36  CGCG                 CG^CG
             1181  CGCG                
ApaI          699  GGGCCC               GGGCC^C
             1500  GGGCCC              
AspHI         393  GTGCTC               G[AT]GC[AT]^C
             1044  GTGCTC              
AspI          111  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              202  GACGTTGTC           
BalI           20  TGGCCA               TGG^CCA
              515  TGGCCA              
BanI           95  GGTGCC               G^G[CT][GA]CC
             1514  GGTGCC              
BbsI          108  GAAGAC               GAAGAC(2/6)
              273  GAAGAC              
BfaI           43  CTAG                 C^TAG
              433  CTAG                
BglI           26  GCCTCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              862  GCCAAAGTGGC         
BpuAI         108  GAAGAC               GAAGAC(2/6)
              273  GAAGAC              
BsaAI        1095  CACGTA               [CT]AC^GT[GA]
             1204  CACGTG              
BsiHKAI       393  GTGCTC               G[AT]GC[AT]^C
             1044  GTGCTC              
BsmAI        1528  GAGAC                GTCTC(1/5)
              417  GTCTC               
BsmI           70  GAATGC               GAATGC(1/-1)
              602  GCATTC              
BspLU11I      302  ACATGT               A^CATGT
              672  ACATGT              
BsrDI        1558  CATTGC               GCAATG(2/0)
             1544  GCAATG              
BstUI          36  CGCG                 CG^CG
             1181  CGCG                
CfoI           35  GCGC                 GCG^C
              805  GCGC                
EarI           10  CTCTTC               CTCTTC(1/4)
             1072  GAAGAG              
HapII          47  CCGG                 C^CGG
               52  CCGG                
HgaI         1027  GACGC                GACGC(5/10)
              115  GCGTC               
HhaI           35  GCGC                 GCG^C
              805  GCGC                
HinP1I         35  GCGC                 G^CGC
              805  GCGC                
HpaII          47  CCGG                 C^CGG
               52  CCGG                
Ksp632I        10  CTCTTC               CTCTTC(1/4)
             1072  GAAGAG              
MaeI           43  CTAG                 C^TAG
              433  CTAG                
MluNI          20  TGGCCA               TGG^CCA
              515  TGGCCA              
MscI           20  TGGCCA               TGG^CCA
              515  TGGCCA              
MseI          362  TTAA                 T^TAA
              914  TTAA                
MspA1I        215  CAGCTG               C[AC]G^C[GT]G
              457  CAGCTG              
MspI           47  CCGG                 C^CGG
               52  CCGG                
MvnI           36  CGCG                 CG^CG
             1181  CGCG                
NdeI          192  CATATG               CA^TATG
              717  CATATG              
PflFI         111  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              202  GACGTTGTC           
PflMI         657  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1080  CCAGGAGGTGG         
PstI          145  CTGCAG               CTGCA^G
             1326  CTGCAG              
PvuII         215  CAGCTG               CAG^CTG
              457  CAGCTG              
SfaNI         768  GATGC                GCATC(5/9)
              837  GATGC               
SfcI          145  CTGCAG               C^T[GA][CT]AG
             1326  CTGCAG              
Tru9I         362  TTAA                 T^TAA
              914  TTAA                
Tth111I       111  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              202  GACGTTGTC           
Van91I        657  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1080  CCAGGAGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
AvaII       BanII       BbvI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       StyI        TaqI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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