Restriction map for pF1KB5019
 (Linear) (Six-base) MAPSORT of: KIBB5019.seq  Check: 974  from: 1  to: 1515



KIBB5019.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     325    1515 
   Size:        325    1190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     389    1102    1114    1515 
   Size:        328      61     713      12     401

  Fragments arranged by size: 

                 713     401     328      61      12
 
AfeI AGC'GCT

Cuts at:      0      20    1515 
   Size:         20    1495
 
ApoI r'AATT_y

Cuts at:      0     430     439     865    1152    1198    1515 
   Size:        430       9     426     287      46     317

  Fragments arranged by size: 

                 430     426     317     287      46       9
 
AvaI C'yCGr_G

Cuts at:      0      27    1515 
   Size:         27    1488
 
AvrII C'CTAG_G

Cuts at:      0     352    1515 
   Size:        352    1163
 
BanII G_rGCy'C

Cuts at:      0     130    1241    1515 
   Size:        130    1111     274

  Fragments arranged by size: 

                1111     274     130
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     752    1515 
   Size:        752     763
 
BlpI GC'TnA_GC

Cuts at:      0    1267    1515 
   Size:       1267     248
 
Bme1580I G_kGCm'C

Cuts at:      0     884    1515 
   Size:        884     631
 
BmgBI CAC'GTC

Cuts at:      0      78    1515 
   Size:         78    1437
 
BmtI G_CTAG'C

Cuts at:      0     908    1515 
   Size:        908     607
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     519    1041    1515 
   Size:        519     522     474

  Fragments arranged by size: 

                 522     519     474
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     855     980    1066    1255    1352    1515 
   Size:        855     125      86     189      97     163

  Fragments arranged by size: 

                 855     189     163     125      97      86
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     552    1515 
   Size:        552     963
 
BsaBI GATnn'nnATC

Cuts at:      0    1076    1515 
   Size:       1076     439
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1228    1258    1515 
   Size:       1228      30     257

  Fragments arranged by size: 

                1228     257      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1033    1515 
   Size:       1033     482
 
BseYI C'CCAG_C

Cuts at:      0     877    1515 
   Size:        877     638
 
BsiHKAI G_wGCw'C

Cuts at:      0     955    1515 
   Size:        955     560
 
Bsp1286I G_dGCh'C

Cuts at:      0     130     884     955    1241    1515 
   Size:        130     754      71     286     274

  Fragments arranged by size: 

                 754     286     274     130      71
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     325    1515 
   Size:        325    1190
 
BsrDI GCAATG_nn'

Cuts at:      0    1125    1515 
   Size:       1125     390
 
BstYI r'GATC_y

Cuts at:      0    1509    1515 
   Size:       1509       6
 
BtgI C'CryG_G

Cuts at:      0      50    1515 
   Size:         50    1465
 
BtsI GCAGTG_nn'

Cuts at:      0     174     254     953    1515 
   Size:        174      80     699     562

  Fragments arranged by size: 

                 699     562     174      80
 
EaeI y'GGCC_r

Cuts at:      0    1407    1515 
   Size:       1407     108
 
EarI CTCTTCn'nnn_

Cuts at:      0    1078    1515 
   Size:       1078     437
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     389     855     980    1066    1102    1114    1255 
   Size:        328      61     466     125      86      36      12     141

Cuts at:   1255    1352    1515 
   Size:         97     163

  Fragments arranged by size: 

                 466     328     163     141     125      97      86      61
                  36      12
 
EcoRV GAT'ATC

Cuts at:      0     298    1515 
   Size:        298    1217
 
HaeII r_GCGC'y

Cuts at:      0      22      40    1515 
   Size:         22      18    1475

  Fragments arranged by size: 

                1475      22      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     590     622    1515 
   Size:        112      32     446      32     893

  Fragments arranged by size: 

                 893     446     112      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     143     325     851    1515 
   Size:         98      45     182     526     664

  Fragments arranged by size: 

                 664     526     182      98      45
 
MslI CAynn'nnrTG

Cuts at:      0     560    1515 
   Size:        560     955
 
MspA1I CmG'CkG

Cuts at:      0      52      67    1515 
   Size:         52      15    1448

  Fragments arranged by size: 

                1448      52      15
 
NdeI CA'TA_TG

Cuts at:      0    1332    1515 
   Size:       1332     183
 
NheI G'CTAG_C

Cuts at:      0     904    1515 
   Size:        904     611
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     833    1515 
   Size:        833     682
 
PfoI T'CCnGG_A

Cuts at:      0     707     869    1515 
   Size:        707     162     646

  Fragments arranged by size: 

                 707     646     162
 
PstI C_TGCA'G

Cuts at:      0     210    1061    1515 
   Size:        210     851     454

  Fragments arranged by size: 

                 851     454     210
 
SacII CC_GC'GG

Cuts at:      0      53    1515 
   Size:         53    1462
 
SbfI CC_TGCA'GG

Cuts at:      0    1061    1515 
   Size:       1061     454
 
SfcI C'TryA_G

Cuts at:      0     206     464     899    1057    1515 
   Size:        206     258     435     158     458

  Fragments arranged by size: 

                 458     435     258     206     158
 
SmaI CCC'GGG

Cuts at:      0      29    1515 
   Size:         29    1486
 
SmlI C'TyrA_G

Cuts at:      0     531    1515 
   Size:        531     984
 
StyI C'CwwG_G

Cuts at:      0     352    1210    1515 
   Size:        352     858     305

  Fragments arranged by size: 

                 858     352     305
 
XmaI C'CCGG_G

Cuts at:      0      27    1515 
   Size:         27    1488
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     ApoI     AvaI    AvrII    BanII     BbsI
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaBI
    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrDI    BstYI
     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoRV    HaeII    Hin4I
     MmeI     MslI   MspA1I     NdeI     NheI    PflMI     PfoI     PstI
    SacII     SbfI     SfcI     SmaI     SmlI     StyI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BmrI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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