Restriction map for pF1KB4185
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BmyI        BpuAI       BsaAI       BsaBI       
BsaI        BsiHKAI     BsiWI       BsmAI       BsmBI       
Bsp1286I    BspDI       BspLU11I    BspMI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoT14I     FbaI        FseI        
FspI        HindIII     HpaI        KasI        KpnI        
MamI        MfeI        MflI        MluI        MseI        
MslI        MunI        NarI        NciI        NcoI        
NdeI        NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII         285  GACGTC               GACGT^C
AccI           88  GTCTAC               GT^[AC][GT]AC
AccIII        923  TCCGGA               T^CCGGA
AcsI          850  GAATTT               [GA]^AATT[CT]
AlwI           16  GGATC                GGATC(4/5)
AlwNI         766  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          850  GAATTT               [GA]^AATT[CT]
AvaI           69  CTCGGG               C^[CT]CG[GA]G
BalI          539  TGGCCA               TGG^CCA
BfaI          682  CTAG                 C^TAG
BseAI         923  TCCGGA               T^CCGGA
BseRI         334  GAGGAG               GAGGAG(10/8)
BsiEI          60  CGACCG               CG[GA][CT]^CG
BsmFI         514  GGGAC                GGGAC(10/14)
BsmI          927  GAATGC               GAATGC(1/-1)
BsoBI          69  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1136  TGTACA               T^GTACA
BspEI         923  TCCGGA               T^CCGGA
BspHI         581  TCATGA               T^CATGA
BsrFI         381  GCCGGC               [GA]^CCGG[CT]
BsrGI        1136  TGTACA               T^GTACA
Cfr10I        381  GCCGGC               [GA]^CCGG[CT]
DraIII        896  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          577  CTCTTC               CTCTTC(1/4)
EcoT22I       867  ATGCAT               ATGCA^T
FauI          273  GCGGG                CCCGC(4/6)
HaeII         369  GGCGCT               [GA]GCGC^[CT]
HincII        241  GTCAAC               GT[CT]^[GA]AC
HindII        241  GTCAAC               GT[CT]^[GA]AC
Ksp632I       577  CTCTTC               CTCTTC(1/4)
MaeI          682  CTAG                 C^TAG
MluNI         539  TGGCCA               TGG^CCA
MroI          923  TCCGGA               T^CCGGA
MscI          539  TGGCCA               TGG^CCA
NaeI          381  GCCGGC               GCC^GGC
NgoMIV        381  GCCGGC               G^CCGGC
NsiI          867  ATGCAT               ATGCA^T
PleI          547  GAGTC                GAGTC(4/5)
PshAI         144  GACCTATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          581  TCATGA               T^CATGA
SfcI         1070  CTATAG               C^T[GA][CT]AG
SspBI        1136  TGTACA               T^GTACA
TaqI          819  TCGA                 T^CGA
TthHB8I       819  TCGA                 T^CGA
XcmI          501  CCATGAAGTGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI          256  GACGCC               G[GA]^CG[CT]C
              285  GACGTC              
AfaI          495  GTAC                 GT^AC
             1137  GTAC                
AvaII         143  GGACC                G^G[AT]CC
              515  GGACC               
BlpI          478  GCTCAGC              GC^T[ACGT]AGC
              961  GCTCAGC             
Bpu1102I      478  GCTCAGC              GC^T[ACGT]AGC
              961  GCTCAGC             
BsaHI         256  GACGCC               G[GA]^CG[CT]C
              285  GACGTC              
BsgI          207  GTGCAG               GTGCAG(16/14)
              262  GTGCAG              
BsrBI         271  GAGCGG               CCGCTC(-3/-3)
              454  GAGCGG              
BsrDI         939  CATTGC               GCAATG(2/0)
             1103  GCAATG              
BssSI         268  CACGAG               CACGAG(-5/-1)
              466  CTCGTG              
CelII         478  GCTCAGC              GC^T[ACGT]AGC
              961  GCTCAGC             
DpnII          17  GATC                 ^GATC
              780  GATC                
EaeI          431  TGGCCG               [CT]^GGCC[GA]
              539  TGGCCA              
HgaI          192  GACGC                GACGC(5/10)
              256  GACGC               
Hin1I         256  GACGCC               G[GA]^CG[CT]C
              285  GACGTC              
HphI          748  GGTGA                GGTGA(8/7)
              392  TCACC               
KspI          395  CCGCGG               CCGC^GG
              434  CCGCGG              
MaeII         286  ACGT                 A^CGT
             1050  ACGT                
MboI           17  GATC                 ^GATC
              780  GATC                
NdeII          17  GATC                 ^GATC
              780  GATC                
PpuMI         142  AGGACCT              [GA]G^G[AT]CC[CT]
              514  GGGACCC             
RsaI          495  GTAC                 GT^AC
             1137  GTAC                
SacII         395  CCGCGG               CCGC^GG
              434  CCGCGG              
Sau3AI         17  GATC                 ^GATC
              780  GATC                
TfiI          559  GAATC                G^A[AT]TC
              760  GATTC               
Tsp509I       851  AATT                 ^AATT
             1113  AATT                
TspRI         205  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1146  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BpmI        
BsaJI       BsaWI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EcoO109I    EcoRII      EcoRV       Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MaeIII      MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NlaIII      NlaIV       PvuII       Sau96I      ScrFI       
SfaNI       TseI        Tsp45I      
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