Restriction map for pF1KB4033
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

Acc65I      AccII       AccIII      AflIII      AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvrII       BanII       BbeI        BcgI        
BcnI        BlnI        BlpI        Bpu1102I    BsaBI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstUI       BstZ17I     CelII       ClaI        
CpoI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       FseI        
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PmeI        
PshAI       PshBI       PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AclI         1996  AACGTT               AA^CGTT
AflII        1945  CTTAAG               C^TTAAG
AgeI         2078  ACCGGT               A^CCGGT
AocI         1278  CCTAAGG              CC^T[ACGT]AGG
Asp700I       829  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         2960  CTCGGG               C^[CT]CG[GA]G
AviII        2874  TGCGCA               TGC^GCA
BbrPI        1355  CACGTG               CAC^GTG
BbvCI         988  GCTGAGG              CCTCAGC(-5/-2)
BfrI         1945  CTTAAG               C^TTAAG
BglI         2722  GCCTTTAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1355  CACGTG               [CT]AC^GT[GA]
BsaWI        2078  ACCGGT               [AT]^CCGG[AT]
BsgI         2459  GTGCAG               GTGCAG(16/14)
BsoBI        2960  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     2843  TGTACA               T^GTACA
BsrDI        1668  CATTGC               GCAATG(2/0)
BsrFI        2078  ACCGGT               [GA]^CCGG[CT]
BsrGI        2843  TGTACA               T^GTACA
BstAPI       2993  GCAAAAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2627  TTCGAA               TT^CGAA
Bsu36I       1278  CCTAAGG              CC^T[ACGT]AGG
Cfr10I       2078  ACCGGT               [GA]^CCGG[CT]
DrdI          994  GACTATCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1278  CCTAAGG              CC^T[ACGT]AGG
EcoRV        2584  GATATC               GAT^ATC
EcoT22I      1437  ATGCAT               ATGCA^T
FspI         2874  TGCGCA               TGC^GCA
HaeII         907  GGCGCT               [GA]GCGC^[CT]
HapII        2079  CCGG                 C^CGG
HgaI         2709  GCGTC                GACGC(5/10)
HpaII        2079  CCGG                 C^CGG
MspA1I       1239  CAGCTG               C[AC]G^C[GT]G
MspI         2079  CCGG                 C^CGG
NdeI         3242  CATATG               CA^TATG
NsiI         1437  ATGCAT               ATGCA^T
NspV         2627  TTCGAA               TT^CGAA
PinAI        2078  ACCGGT               A^CCGGT
PmaCI        1355  CACGTG               CAC^GTG
PmlI         1355  CACGTG               CAC^GTG
PpuMI        1304  GGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I     1996  AACGTT               AA^CGTT
PvuII        1239  CAGCTG               CAG^CTG
ScaI         3499  AGTACT               AGT^ACT
SfuI         2627  TTCGAA               TT^CGAA
SmlI         1945  CTTAAG               C^T[CT][GA]AG
SphI         1595  GCATGC               GCATG^C
SspBI        2843  TGTACA               T^GTACA
StuI          507  AGGCCT               AGG^CCT
XmnI          829  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AatII        1939  GACGTC               GACGT^C
             2739  GACGTC              
AccI          396  GTCTAC               GT^[AC][GT]AC
             2643  GTCTAC              
AcyI         1939  GACGTC               G[GA]^CG[CT]C
             2739  GACGTC              
AlwNI        1380  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
             3529  CAGCTCCTG           
BamHI        1543  GGATCC               G^GATCC
             2084  GGATCC              
BciVI         453  GTATCC               GTATCC(6/5)
             2532  GTATCC              
BglII        1481  AGATCT               A^GATCT
             1488  AGATCT              
BsaHI        1939  GACGTC               G[GA]^CG[CT]C
             2739  GACGTC              
BsaI         2507  GAGACC               GGTCTC(1/5)
             3691  GAGACC              
CfoI          908  GCGC                 GCG^C
             2875  GCGC                
DraI         1918  TTTAAA               TTT^AAA
             3333  TTTAAA              
DraII         868  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1304  GGGTCCC             
DraIII       1529  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3452  CACTTTGTG           
EcoO109I      868  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1304  GGGTCCC             
FauI          181  GCGGG                CCCGC(4/6)
             1935  GCGGG               
HhaI          908  GCGC                 GCG^C
             2875  GCGC                
Hin1I        1939  GACGTC               G[GA]^CG[CT]C
             2739  GACGTC              
HinP1I        908  GCGC                 G^CGC
             2875  GCGC                
MfeI          365  CAATTG               C^AATTG
             2193  CAATTG              
MunI          365  CAATTG               C^AATTG
             2193  CAATTG              
NcoI          261  CCATGG               C^CATGG
             1963  CCATGG              
NspI          550  GCATGT               [GA]CATG^[CT]
             1595  GCATGC              
PstI         1658  CTGCAG               CTGCA^G
             3464  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BalI        BanI        BbsI        
BbvI        BclI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeIII      HincII      HindII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       
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