Restriction map for pF1KB3125
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbvCI       BclI        BglI        BlnI        BlpI        
Bpu1102I    BsaI        BsmBI       BsmI        BspDI       
BspHI       BsrFI       BssHII      BssSI       BstBI       
BstEII      BstPI       CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MluNI       MscI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TfiI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI         1265  GTATAC               GT^[AC][GT]AC
AccII          42  CGCG                 CG^CG
AccIII       1697  TCCGGA               T^CCGGA
AflII         688  CTTAAG               C^TTAAG
AocI         1563  CCTCAGG              CC^T[ACGT]AGG
AvaI          484  CCCGAG               C^[CT]CG[GA]G
BanI          754  GGCGCC               G^G[CT][GA]CC
BbeI          754  GGCGCC               GGCGC^C
BbrPI        1372  CACGTG               CAC^GTG
BbsI            6  GTCTTC               GAAGAC(2/6)
BciVI        1297  GGATAC               GTATCC(6/5)
BfrI          688  CTTAAG               C^TTAAG
BpuAI           6  GTCTTC               GAAGAC(2/6)
BsaAI        1372  CACGTG               [CT]AC^GT[GA]
BsaBI        1701  GATGGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1697  TCCGGA               [AT]^CCGG[AT]
BseAI        1697  TCCGGA               T^CCGGA
BsiEI         246  CGACCG               CG[GA][CT]^CG
BsiWI          83  CGTACG               C^GTACG
BsoBI         484  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      770  TGTACA               T^GTACA
BspEI        1697  TCCGGA               T^CCGGA
BsrBI          47  GAGCGG               CCGCTC(-3/-3)
BsrDI         938  GCAATG               GCAATG(2/0)
BsrGI         770  TGTACA               T^GTACA
Bst1107I     1265  GTATAC               GTA^TAC
BstAPI        333  GCAAACCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          42  CGCG                 CG^CG
BstZ17I      1265  GTATAC               GTA^TAC
Bsu36I       1563  CCTCAGG              CC^T[ACGT]AGG
DraIII       1606  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1563  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1746  CCTCATTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1378  GATATC               GAT^ATC
HaeII         754  GGCGCC               [GA]GCGC^[CT]
HgaI            4  GCGTC                GACGC(5/10)
HincII        508  GTTGAC               GT[CT]^[GA]AC
HindII        508  GTTGAC               GT[CT]^[GA]AC
KasI          754  GGCGCC               G^GCGCC
MamI         1701  GATGGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1697  TCCGGA               T^CCGGA
MspA1I        962  CAGCTG               C[AC]G^C[GT]G
MvnI           42  CGCG                 CG^CG
NarI          754  GGCGCC               GG^CGCC
NcoI         1655  CCATGG               C^CATGG
NdeI          644  CATATG               CA^TATG
PflMI         435  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1372  CACGTG               CAC^GTG
PmlI         1372  CACGTG               CAC^GTG
PstI          513  CTGCAG               CTGCA^G
PvuII         962  CAGCTG               CAG^CTG
SacI          469  GAGCTC               GAGCT^C
ScaI          797  AGTACT               AGT^ACT
SexAI          91  ACCTGGT              A^CC[AT]GGT
SfcI          513  CTGCAG               C^T[GA][CT]AG
SfoI          754  GGCGCC               GGC^GCC
SspBI         770  TGTACA               T^GTACA
Van91I        435  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          980  AAATTC               [GA]^AATT[CT]
             1495  GAATTT              
AcyI            3  GGCGTC               G[GA]^CG[CT]C
              754  GGCGCC              
AflIII        635  ACATGT               A^C[GA][CT]GT
              767  ACATGT              
ApoI          980  AAATTC               [GA]^AATT[CT]
             1495  GAATTT              
BanII         130  GGGCTC               G[GA]GC[CT]^C
              469  GAGCTC              
BcgI          246  CGACCGGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               28  GCAACCTCCTCG        
BfaI          299  CTAG                 C^TAG
              598  CTAG                
BglII         137  AGATCT               A^GATCT
              303  AGATCT              
BpmI          493  CTGGAG               CTGGAG(16/14)
              602  CTGGAG              
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
              754  GGCGCC              
BsgI          896  CTGCAC               GTGCAG(16/14)
             1292  GTGCAG              
BspLU11I      635  ACATGT               A^CATGT
              767  ACATGT              
BspMI          68  ACCTGC               ACCTGC(4/8)
              224  ACCTGC              
DsaI          108  CCACGG               C^C[GA][CT]GG
             1655  CCATGG              
EaeI          623  CGGCCA               [CT]^GGCC[GA]
             1365  TGGCCG              
FauI          257  CCCGC                CCCGC(4/6)
               43  GCGGG               
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
              754  GGCGCC              
MaeI          299  CTAG                 C^TAG
              598  CTAG                
MaeIII        869  GTCAC                ^GT[ACGT]AC
             1195  GTCAC               
MslI          405  CACCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1111  CATGTCGATG          
SmlI          133  CTCAAG               C^T[CT][GA]AG
              688  CTTAAG              
Tsp45I        869  GTCAC                ^GT[GC]AC
             1195  GTCAC               
XcmI          404  CCACCAAGGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              567  CCAGCTCTCCACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MboI        
MboII       MflI        MnlI        MseI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
XhoII       
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