Restriction map for pF1KB3125
 (Linear) (Six-base) MAPSORT of: KIBB3125.seq  Check: 2089  from: 1  to: 1782



KIBB3125.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0    1266    1782 
   Size:       1266     516
 
AflII C'TTAA_G

Cuts at:      0     688    1782 
   Size:        688    1094
 
AflIII A'CryG_T

Cuts at:      0     635     767    1782 
   Size:        635     132    1015

  Fragments arranged by size: 

                1015     635     132
 
AleI CACnn'nnGTG

Cuts at:      0     409    1782 
   Size:        409    1373
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1133    1256    1782 
   Size:       1133     123     526

  Fragments arranged by size: 

                1133     526     123
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     214     246    1782 
   Size:        214      32    1536

  Fragments arranged by size: 

                1536     214      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     277     441     504    1779    1782 
   Size:        277     164      63    1275       3

  Fragments arranged by size: 

                1275     277     164      63       3
 
ApoI r'AATT_y

Cuts at:      0     980    1495    1782 
   Size:        980     515     287

  Fragments arranged by size: 

                 980     515     287
 
AvaI C'yCGr_G

Cuts at:      0     484    1782 
   Size:        484    1298
 
BanI G'GyrC_C

Cuts at:      0     754    1782 
   Size:        754    1028
 
BanII G_rGCy'C

Cuts at:      0     134     473    1782 
   Size:        134     339    1309

  Fragments arranged by size: 

                1309     339     134
 
BbeI G_GCGC'C

Cuts at:      0     758    1782 
   Size:        758    1024
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      17      51     235     269    1782 
   Size:         17      34     184      34    1513

  Fragments arranged by size: 

                1513     184      34      34      17
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1291    1782 
   Size:       1291     491
 
BglII A'GATC_T

Cuts at:      0     137     303    1782 
   Size:        137     166    1479

  Fragments arranged by size: 

                1479     166     137
 
Bme1580I G_kGCm'C

Cuts at:      0    1417    1782 
   Size:       1417     365
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     136    1722    1777    1782 
   Size:        136    1586      55       5

  Fragments arranged by size: 

                1586     136      55       5
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     623    1782 
   Size:        514     109    1159

  Fragments arranged by size: 

                1159     514     109
 
Bpu10I CC'TnA_GC

Cuts at:      0    1523    1782 
   Size:       1523     259
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    1782 
   Size:        118    1664
 
BsaAI yAC'GTr

Cuts at:      0    1374    1782 
   Size:       1374     408
 
BsaBI GATnn'nnATC

Cuts at:      0    1705    1782 
   Size:       1705      77
 
BsaHI Gr'CG_yC

Cuts at:      0       4     755    1782 
   Size:          4     751    1027

  Fragments arranged by size: 

                1027     751       4
 
BsaWI w'CCGG_w

Cuts at:      0    1697    1782 
   Size:       1697      85
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     556     586    1782 
   Size:        556      30    1196

  Fragments arranged by size: 

                1196     556      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      24     343     361    1768    1782 
   Size:         24     319      18    1407      14

  Fragments arranged by size: 

                1407     319      24      18      14
 
BseYI C'CCAG_C

Cuts at:      0     889    1716    1782 
   Size:        889     827      66

  Fragments arranged by size: 

                 889     827      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     881    1313    1782 
   Size:        881     432     469

  Fragments arranged by size: 

                 881     469     432
 
BsiEI CG_ry'CG

Cuts at:      0     249    1782 
   Size:        249    1533
 
BsiHKAI G_wGCw'C

Cuts at:      0     473    1240    1529    1782 
   Size:        473     767     289     253

  Fragments arranged by size: 

                 767     473     289     253
 
BsiWI C'GTAC_G

Cuts at:      0      83    1782 
   Size:         83    1699
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     473    1240    1417    1529    1782 
   Size:        134     339     767     177     112     253

  Fragments arranged by size: 

                 767     339     253     177     134     112
 
BspEI T'CCGG_A

Cuts at:      0    1697    1782 
   Size:       1697      85
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      77     233    1782 
   Size:         77     156    1549

  Fragments arranged by size: 

                1549     156      77
 
BsrBI CCG'CTC

Cuts at:      0      49    1782 
   Size:         49    1733
 
BsrDI GCAATG_nn'

Cuts at:      0     945    1782 
   Size:        945     837
 
BsrGI T'GTAC_A

Cuts at:      0     770    1782 
   Size:        770    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     339    1782 
   Size:        339    1443
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     126     168     414     582    1782 
   Size:        126      42     246     168    1200

  Fragments arranged by size: 

                1200     246     168     126      42
 
BstYI r'GATC_y

Cuts at:      0     137     303     500    1776    1782 
   Size:        137     166     197    1276       6

  Fragments arranged by size: 

                1276     197     166     137       6
 
BstZ17I GTA'TAC

Cuts at:      0    1267    1782 
   Size:       1267     515
 
Bsu36I CC'TnA_GG

Cuts at:      0    1564    1782 
   Size:       1564     218
 
BtgI C'CryG_G

Cuts at:      0     108    1655    1782 
   Size:        108    1547     127

  Fragments arranged by size: 

                1547     127     108
 
BtsI GCAGTG_nn'

Cuts at:      0    1240    1782 
   Size:       1240     542
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     419     454    1782 
   Size:        419      35    1328

  Fragments arranged by size: 

                1328     419      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1611    1782 
   Size:       1611     171
 
EaeI y'GGCC_r

Cuts at:      0     623    1365    1782 
   Size:        623     742     417

  Fragments arranged by size: 

                 742     623     417
 
EarI CTCTTCn'nnn_

Cuts at:      0     167     172     856    1309    1782 
   Size:        167       5     684     453     473

  Fragments arranged by size: 

                 684     473     453     167       5
 
EcoICRI GAG'CTC

Cuts at:      0     471    1782 
   Size:        471    1311
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     623    1782 
   Size:        514     109    1159

  Fragments arranged by size: 

                1159     514     109
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1750    1782 
   Size:       1750      32
 
EcoRV GAT'ATC

Cuts at:      0    1380    1782 
   Size:       1380     402
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1707    1782 
   Size:       1707      75
 
HaeII r_GCGC'y

Cuts at:      0     758    1782 
   Size:        758    1024
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     196     228     381     413     729     761    1782 
   Size:        196      32     153      32     316      32    1021

  Fragments arranged by size: 

                1021     316     196     153      32      32      32
 
HincII GTy'rAC

Cuts at:      0     510    1782 
   Size:        510    1272
 
KasI G'GCGC_C

Cuts at:      0     754    1782 
   Size:        754    1028
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     690     788    1736    1782 
   Size:        690      98     948      46

  Fragments arranged by size: 

                 948     690      98      46
 
MslI CAynn'nnrTG

Cuts at:      0     409    1115    1782 
   Size:        409     706     667

  Fragments arranged by size: 

                 706     667     409
 
MspA1I CmG'CkG

Cuts at:      0     964    1782 
   Size:        964     818
 
NarI GG'CG_CC

Cuts at:      0     755    1782 
   Size:        755    1027
 
NcoI C'CATG_G

Cuts at:      0    1655    1782 
   Size:       1655     127
 
NdeI CA'TA_TG

Cuts at:      0     645    1782 
   Size:        645    1137
 
NspI r_CATG'y

Cuts at:      0     639     771    1114    1782 
   Size:        639     132     343     668

  Fragments arranged by size: 

                 668     639     343     132
 
PciI A'CATG_T

Cuts at:      0     635     767    1782 
   Size:        635     132    1015

  Fragments arranged by size: 

                1015     635     132
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     441    1782 
   Size:        441    1341
 
PfoI T'CCnGG_A

Cuts at:      0     350     803    1147    1782 
   Size:        350     453     344     635

  Fragments arranged by size: 

                 635     453     350     344
 
PmlI CAC'GTG

Cuts at:      0    1374    1782 
   Size:       1374     408
 
PstI C_TGCA'G

Cuts at:      0     517    1782 
   Size:        517    1265
 
PvuII CAG'CTG

Cuts at:      0     964    1782 
   Size:        964     818
 
SacI G_AGCT'C

Cuts at:      0     473    1782 
   Size:        473    1309
 
ScaI AGT'ACT

Cuts at:      0     799    1782 
   Size:        799     983
 
SexAI A'CCwGG_T

Cuts at:      0      91    1782 
   Size:         91    1691
 
SfcI C'TryA_G

Cuts at:      0     513    1782 
   Size:        513    1269
 
SfoI GGC'GCC

Cuts at:      0     756    1782 
   Size:        756    1026
 
SmlI C'TyrA_G

Cuts at:      0     133     688    1782 
   Size:        133     555    1094

  Fragments arranged by size: 

                1094     555     133
 
StyI C'CwwG_G

Cuts at:      0     407    1272    1655    1674    1782 
   Size:        407     865     383      19     108

  Fragments arranged by size: 

                 865     407     383     108      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     443    1782 
   Size:        443    1339
 
TatI w'GTAC_w

Cuts at:      0     770     797    1782 
   Size:        770      27     985

  Fragments arranged by size: 

                 985     770      27
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     472     504     534     566    1782 
   Size:        472      32      30      32    1216

  Fragments arranged by size: 

                1216     472      32      32      30
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     411     574    1782 
   Size:        411     163    1208

  Fragments arranged by size: 

                1208     411     163
 

 Enzymes that do cut:

     AccI    AflII   AflIII     AleI     AlfI     AloI    AlwNI     ApoI
     AvaI     BanI    BanII     BbeI     BcgI    BciVI    BglII Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BstAPI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI    CspCI   DraIII     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI    EcoRV     FalI    HaeII    Hin4I   HincII
     KasI     MmeI     MslI   MspA1I     NarI     NcoI     NdeI     NspI
     PciI    PflMI     PfoI     PmlI     PstI    PvuII     SacI     ScaI
    SexAI     SfcI     SfoI     SmlI     StyI    TaqII     TatI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI     AgeI     AhdI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI
     BplI     BsaI     BsmI    BsmBI    BspHI    BsrFI   BssHII    BssSI
    BstBI   BstEII    BtgZI     ClaI     DraI     DrdI     EagI     EciI
 EcoO109I    EcoRI     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     NaeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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